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190 results about "Carbohydrate chains" patented technology
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Carbohydrate Chains, or glycolipids/glycoproteins, are chains found on the surface of the cell membrane that are made of carbohydrates + lipid (or) protein. The primary function of these chains is to recognize harmful cells (cell-cell recognition).
Medical composition containing photocrosslinkable chitosan derivative
InactiveUS20050238702A1Promote tissue regenerationFree from riskPowder deliveryOrganic active ingredientsSide effectMedicine
A medical composition is provided which is advantageous as a sealant for wound openings as well as have a function promoting healing of intractable wound or incision wound, and which accelerate tissue regeneration but does not cause any side effect such as canceration. The present invention relates to a medical composition comprising a photo-crosslinkable chitosan derivative and a wound healing promoter. The photo-crosslinkable chitosan derivative is preferably a polymer obtainable by incorporating a carbohydrate chain containing a reducing terminal to at least one portion of amino groups of chitosan back bone and incorporating a photoreactive group to at least another part of the amino groups. The wound healing promoter is preferably a growth factor.
Owner:NETECH
Method for detecting low-molecular-weight heparin by combining ion pair reversed phase chronmatogaphy and mass spectrum
ActiveCN102759596AImprove detection levelEnsure safetyComponent separationChromatographic separationMass spectrometry
The invention relates to a method for detecting low-molecular-weight heparin by combining ion pair reversed phase chronmatogaphy and mass spectrum. The method comprises the steps of: detecting all compositions of low-molecular-weight heparin by combining ion pair reversed phase chronmatogaphy and high-resolution mass spectrum, separating main compositions from a sample through the ion pair reversed phase chronmatogaphy, obtaining precise molecular weight by the high-resolution mass spectrum, and calculating information of a carbohydrate chain sequence, including structures of two ends, length of a carbohydrate chain and substitution number of ethanoyl and sulfate, thus carrying out fine surface features on the low-molecular-weight heparin. The method has great practical values on improving the heparin detection level and ensuring drug safety.
Owner:HEBEI CHANGSHAN BIOCHEM PHARMA
Α-1,3-fucosyltransferase
The invention provides a novel species of α-1,3-fucosyltransferase expressed by a gene cloned from animal cells, a cDNA coding for the α-1,3-fucosyltransferase, a method of detecting, or inhibiting the production of, the α-1,3-fucosyltransferase which involves the use of the cDNA, a recombinant vector containing the DNA as an insert, a cell harboring the recombinant vector, and a method of producing same. The α-1,3-fucosyltransferase of the invention is useful in the production of carbohydrate chains having useful physiological activity, for example sialyl Lewis x, and modifications thereof.
Owner:KYOWA HAKKO KIRIN CO LTD
Humanization modified anti-CD147 chimeric antibody HcHAb18 and application thereof
ActiveCN104086654AAbility to inhibit invasion and migrationGrowth inhibitory effectImmunoglobulins against cell receptors/antigens/surface-determinantsAntibody ingredientsAntibody-dependent cell-mediated cytotoxicityChimeric antibody
The invention provides a humanized modified anti-CD147 chimeric antibody HcHAb18 and an application thereof. The anti-CD147 human-rat chimeric antibody is prepared on the basis of obtaining hybridoma cells of an anti-human CD147 monoclonal antibody and antibody HcHAb18 genes of the hybridoma cells, wherein the variable region of light chains of the anti-CD147 human-rat chimeric antibody has the amino acid sequence shown in SEQ ID NO:1; the variable region of heavy chains of the anti-CD147 human-rat chimeric antibody has the amino acid sequence shown in SEQ ID NO:2; fucose are not combined in N-acetylglucosamine in a reducing end of a carbohydrate chain at a Fc segment of the anti-CD147 monoclonal antibody in a specific host cell; the glycoform of the anti-CD147 monoclonal antibody is of a mannose type (MAN 5); the anti-CD147 chimeric antibody has antibody dependent cell-mediated cytotoxicity (ADCC). The invention further provides an expression vector and an engineering cell strain of the anti-CD147 chimeric antibody and the application of the anti-CD147 chimeric antibody taken as a cancer treating medicine.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Lectin test chip for saliva sample, and treatment method thereof
InactiveCN103336126AEasy to prepareEasy to handlePeptide librariesBiological testingSaliva sampleGlycoprotein i
The invention provides a lectin test chip for a saliva sample, wherein a production cost is low, test targeting is strong, inherent advantages of a lectin chip are combined, and rapid, simple and efficient detection can be performed so as to obtain intermediate result information (saliva whole protein N-glycosylation and O-glycosylation carbohydrate chain structure information) adopted as type 2 diabetes mellitus evaluation. The lectin test chip adopts an epoxidation film base, and is characterized in that at least one of lectins such as PTL-I, LCA and SBA is immobilized on the epoxidation film base in a spotting immobilization manner. According to the present invention, the preparation method and the treatment method of the lectin test chip are optimized so as to establish the optimal reaction system for production of whole protein N-glycosylation and O-glycosylation information for saliva sample detection, and provide convenience for rapid and efficient detection of saliva glycoprotein carbohydrate chain structures of type 2 diabetes mellitus patients.
Owner:NORTHWEST UNIV(CN)
Glycosyl transferases and applications of glycosyl transferases
ActiveCN104232723ASpecific and efficient transferHas anticancer activityTransferasesFermentationTriterpeneCoa transferase
The invention relates to a group of glycosyl transferases and applications of the glycosyl transferases. Particularly, the invention provides applications of glycosyl transferases gGT25, gGT13, gGT30, gGT25-1, gGT25-3, gGT25-5, gGT29, gGT29-3, gGT29-4, gGT29-5, gGT29-6, gGT29-7, 3GT1, 3GT2, 3GT3 and 3GT4, and derived peptides of the glycosyl transferases in glycosylation catalysis and new saponin synthesis of terpenoids. The glycosyl transferases can specifically and efficiently catalyze hydroxyl glycosylation of C-20 bit and / or C-6 bit and / or C-3 of a tetracyclic triterpenoid compound substrate, and / or transfers the glycosyl groups from glycosyl donors to the first glycosyl groups of the C-3 bit and C-6 bit of the tetracyclic triterpenoid compound to extend a carbohydrate chain. The glycosyl transferases disclosed by the invention can also be applied to building synthetic rare ginsenosides and a plurality of novel ginsenosides and derivatives of the ginsenosides.
Owner:SYNBIOTECH (SUZHOU) CO LTD
Agglutinin chip for identifying breast cancer based on sialoprotein, reagent kit and application of reagent kit
The relates to an agglutinin chip used for identifying cancer, in particular to an agglutinin chip for identifying breast cancer based on sialoprotein, a reagent kit and application of the reagent kit, and aims at providing an agglutinin chip for identifying breast cancer in a non-destructive mode through the change of carbohydrate chains in saliva, a reagent kit and application of the reagent kit. The agglutinin chip for identifying breast cancer based on sialoprotein comprises an agglutinin testing probe set. The agglutinin testing probe set at least comprises a combination of PHA-E+L, LTL, BS-I, MAL-I, LCA, BPL, PTL-II, NPA and GSL-I, or at least comprises a combination of LTL, BS-I, MAL-I, LCA, BPL, NPA and GSL-I. By means of the agglutinin chip, differentially-expressed glycoprotein carbohydrate chain structures in a patient saliva sample can be rapidly detected, whether a corresponding person suffers from a breast tumor or not is detected, and whether the breast tumor is breast cancer or not is determined; besides, saliva is adopted as an object to be detected and is easy and convenient to collect, and health risks existing when a serum sample is collected for detection in the prior art are avoided.
Owner:深圳格道糖生物技术有限公司
Separation and purification method of lycium ruthenicum polysaccharide and five polysaccharides obtained through separation
The invention relates to a separation and purification method of a lycium ruthenicum polysaccharide and five polysaccharides obtained through separation. Lycium ruthenicum fruits serve as raw materials and a polysaccharide purified product is obtained through extraction by means of ultrasound heating, depoteinization by trichloroacetic acid, decoloration by hydrogen peroxide, diethyl-aminoethanol (DEAE) cellulose column chromatography, Sephadex G-100 column chromatography and the like. According to structure analysis, the lycium ruthenicum polysaccharide is arabinogalactan in multiple branches. The extraction process is performed under a relatively moderate condition and a carbohydrate chain portion on polysaccharide molecules is conserved completely. After purification, five different polysaccharide components are obtained. The five polysaccharides obtained through separation are clear in structure, controllable in quality and significant in the fields of food, cosmetic, health care products, medicines and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Lectin simulant preparation method and application based on molecular imprinting technique
ActiveCN104820100AEasy to makeLow costBiological material analysisPeptide preparation methodsDiseaseEnzyme digestion
The invention discloses a lectin simulant preparation method based on a molecular imprinting technique. The preparation method includes the steps: firstly, acquiring a carbohydrate chain on complete glycoprotein as a template molecule by enzyme digestion reaction; secondly, fixing the template molecule on the surface of a boric acid functional substrate material by the aid of a boric affinity function; thirdly, performing condensation reaction by the aid of silanization reagents to form an imprinting layer; finally, removing a template under the acid conditions to form an imprinting cavity to obtain a lectin simulant based on molecular imprinting. The lectin simulant is simple to prepare, low in cost, stable in nature, excellent in specificity and affinity and high in anti-jamming capacity, a recognized target object is more easily released, integrated glycoprotein can be recognized, characteristic fragments such as glycopeptides and glycan of the glycoprotein can be recognized, and the lectin simulant still can keep single-minded recognized capacity in used for complex biological samples and has an excellent application prospect in aspects such as glycoproteomics, metabonomics, glycobiology, disease diagnosis and analeptic inspection.
Owner:NANJING UNIV
Membrane assisted separation of glycoprotein all N-linked carbohydrate chain and identification method thereof
InactiveCN102788720AAvoid bringing inClearly separate sugar chain effectPreparing sample for investigationMaterial analysis by electric/magnetic meansChemical reactionCell separation
The invention provides a membrane assisted separation of glycoprotein all N-linked carbohydrate chain and an identification method thereof, and solves problems of low reaction efficiency and identification of few types of carbohydrates in prior art. According to the invention, a molecular sieve effect of a membrane of 8-12KD is employed to separate carbohydrate chain released from glycoprotein from protein; on a molecular sieve of 10KD, an N-linked carbohydrate chain of the glycoprotein is released by a PNGase F enzyme; the N-linked carbohydrate chain flows out through repeated centrifugation, but the protein is retained on the membrane, thereby realizing separation of the carbohydrate chain. The method has obvious carbohydrate chain separation effect, and avoids by-products caused by non-specific adsorption and chemical reactions; besides, the method has high reaction efficiency and concise reaction steps.
Owner:NORTHWEST UNIV(CN)
Lectin microarray for detecting carbohydrate chain marker based on sialoprotein and detection method of carbohydrate chain marker using same
The invention relates to a lectin microarray for detecting a carbohydrate chain marker based on sialoprotein and a detection method of the carbohydrate chain marker using the same. The lectin microarray can nondestructively detect the carbohydrate chain marker by distinguishing sialoprotein. The lectin microarray comprises a testing lectin probe group, wherein the testing lectin probe group comprises a vicia villosa lectin, an aleuria aurantia lectin, a pea lectin, a winged bean lectin-II an a winged bean lectin-I, and the winged bean lectin-II and the winged bean lectin-I are used as internal reference lectins. The lectin microarray has the advantages that the human body is not injured when the carbohydrate chain marker for detecting the carbohydrate chain marker is harmless to a human body, less samples are used, the detection speed is high and the sensitivity is high, and the lectin microarray can be applied to identification of stomach cancer.
Owner:深圳格道糖生物技术有限公司
Glycoprotein N-carbohydrate chain one-step enrichment-derivation processing method based on graphene and MALDI-TOF-MS analysis method
ActiveCN104181258AIncrease the areaLarge structureComponent separationChemistryMass spectrum analysis
The invention discloses a glycoprotein N-carbohydrate chain one-step enrichment-derivation processing method based on graphene and an MALDI-TOF-MS analysis method. The chain one-step enrichment-derivation processing method comprises performing enzymatic-hydrolysis release on a glycoprotein N-carbohydrate chain, using graphene and pyrenebutyric hydrazide to performing one-step enrichment and derivation on N-carbohydrate chain, eluting N-carbohydrate chain, performing mass spectrometry, and the like. By utilizing the special pi-pi conjugate interaction of an aromatic compound and graphene and utilizing the specific efficient covalent coupling reaction of an aromatic compound containing a hydrazide or amino functional group and a hemiacetal of the N-carbohydrate chain, one-step enrichment and derivation of N-carbohydrate chain is realized, and efficient derivation of N-carbohydrate chain is finished when specific enrichment is performed. Therefore, the method avoids complicated operation steps, improves sample processing flux and substantially reduces sample loss.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Diagnostic marker for systemic lupus erythematosus
The invention relates to a diagnostic marker for systemic lupus erythematosus, and in particular discloses application of a reagent for specific detection of high mannose type N-linked carbohydrate chain level in a serum antibody to preparation of a diagnostic tool for diagnosis and / or prognosis assessment of systemic lupus erythematosus. The increase of the high mannose type N-linked carbohydrate chain level in the serum antibody is closely related to the occurrence of systemic lupus erythematosus. Therefore, the diagnostic marker can be used for early diagnosis and / or prognostic assessment of systemic lupus erythematosus, and further can be used for guiding treatment of systemic lupus erythematosus.
Owner:杭州中赢生物医疗科技有限公司
Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof
ActiveCN103145864AWidely distributedEasy access toOrganic active ingredientsNervous disorderBiotechnologyCodonopsis pilosula
The invention discloses codonopsis pilosula uniform polysaccharide CPPib which is mainly composed of, by Mole percentage, 44.28% of rhamnose, 20.42% of galactose, 11.32% of arabinose and 23.97% of galacturonic acid, weight-average molecular weight of the codonopsis pilosula uniform polysaccharide CPPib is 1.45*105Da, and main carbohydrate chains are 1,2 connected rhamnose (rha, 45%), 1,4-connected galacturonic acid (GalA, 25%) and 1,2,6 connected galactose (Gal, 30%). A preparation method of the codonopsis pilosula uniform polysaccharide CPPib has the advantages of being simple, scientific, reasonable, and capable of preparing products with very high purity with only need of passing through an anion exchanging column chromatograph once. The prepared products have the advantages of having anti-tumor effect, educational effect, very high clinical application value and healthcare function.
Owner:LANZHOU UNIVERSITY
High-sulfating chondroitin sulfate (CS) and preparing method and application thereof
ActiveCN105924544AShorten the growth cycleIncrease productionOrganic active ingredientsAntineoplastic agentsSulfationChondroitin
The invention relates to a high-sulfating chondroitin sulfate (CS) and a preparing method and application thereof. According to the high-sulfating CS, the molecular weight is 100 KDa to 800 KDa, the sulfating degree is 0.7 to 1.43, and disaccharide compositions are O-units, C-units, A-units and E-units. The high-sulfating CS is a CS which is extracted from cartilage of dosidicus gigas and is rich in E-unit, and is a DG-CS with the specific disaccharide compositions, specific carbohydrate chain arranging and the specific molecular weight. An experiment proves that the high-sulfating CS has quite good anti-tumor metastasis activity.
Owner:SHANDONG UNIV
Method for relatively quantitatively analyzing carbohydrate chain in two-end labeling manner
ActiveCN104020216AIncrease diversityImprove Quantitative AccuracyMaterial analysis by electric/magnetic meansSialic acidIsotope
The invention discloses a method for relatively quantitatively analyzing a carbohydrate chain in a two-end labeling manner, and belongs to the field of quantitative carbohydrate analysis. Amidation on sialic acid and amination on a reduction end of a carbohydrate chain structure are respectively modified by utilizing a hydrazide agent and an amination agent, meanwhile, a sialic acid group of the carbohydrate chain structure is protected, and the reduction end of the carbohydrate chain is labeled by isotope, so that the complete structure information and quantitative information of the carbohydrate chain are simultaneously analyzed, and the comprehensiveness of analysis and the accuracy of the quantitation on the carbohydrate chain are improved.
Owner:BEIJING NANOPEP BIOTECH CORP LTD
Complete glycopeptide identifying method and system
ActiveCN106018535AImprove reliabilityHigh identification accuracyMaterial analysis by electric/magnetic meansCrystallographyComputation complexity
The invention provides a complete glycopeptide identifying method. The method includes the steps the a carbohydrate chain structure database is traversed for any actual measurement tandem mass spectrum to be identified, for each carbohydrate chain structure, the mass of glycopeptide Y ions possibly obtained in fragment tests is concluded according to the mass of parent ions in the current series connection spectrogram, then the number of spectrum peaks matched with the current second-level spectrum is calculated, and the number of the matched spectrum peaks is used as a coarse marking result of matching between the glycopeptide Y ions and the current second-level spectrum; carbohydrate chain structures with the top K coarse marking scores serve as candidate carbohydrate chain structures; for the current series connection spectrogram, all the candidate carbohydrate chain structures are traversed, spectrum-spectrum matching between actual measurement spectrums and theoretical spectrums of peptide fragments of each candidate carbohydrate chain structure is marked, spectrum-spectrum matching between actual measurement spectrums and theoretical spectrums of the carbohydrate chain structures is marked, and then the glycopeptide structure identifying result is obtained. Reliability of complete large-scale glycopeptide identification can be improved, and calculation complexity is low.
Owner:INST OF COMPUTING TECH CHINESE ACAD OF SCI
Toughening forming auxiliary for preparing hard capsule taking starch as matrix and application
ActiveCN103520729AImproves friabilityAvoid disintegration difficultiesPharmaceutical non-active ingredientsCapsule deliverySolubilityCarrageenan
The invention relates to a toughening forming auxiliary of a hard capsule taking starch as a matrix for food, healthcare products, medicines and the like, and a starch hard capsule for the food, healthcare products, medicines and the like, prepared from the toughening forming auxiliary of a hard capsule taking starch as a matrix in combination with a starch matrix. The toughening forming auxiliary for preparing a hard capsule taking starch as a matrix consists of 5-50wt% of carrageenan and 50-95wt% of ghatti gum. According to the toughening forming auxiliary provided by the invention, the toughening forming auxiliary is added into the starch matrix, so that the glue-dip forming is assisted by the gelation effect of the carrageenan, and a final capsule shell is toughened by the net structure generated in gelation and a natural polysaccharide carbohydrate chain structure in the ghatti gum; meanwhile, the disintegration of the hard capsule can be effectively promoted by the good solubility and emulsifiability of the ghatti gum, thus the prepared starch hard capsule has excellent flexibility and can be quickly disintegrated.
Owner:北京崇尚科技开发有限公司 +1
Dipeptide based polymer materials and application of dipeptide based polymer materials in saccharide separation and glycopeptide enrichment
InactiveCN105254707AEasy to separateHigh selectivityPeptide preparation methodsFunctional monomerDipeptide
The invention relates to a screening method of dipeptide functional monomers having excellent separating capacity on saccharide. A dipeptide compound obtained on the basis of screening of standard deviation parameter D and R values is constructed, the dipeptide compound obtained through screening is further coupled with a group containing double bonds, polymerizable dipeptide functional monomers are obtained and are polymerized on surfaces of various matrixes, and a series of polymer modified chromatographic stationary phase materials are obtained. The materials have excellent separating capacity on oligomeric galactose, oligomeric fructose and sialic acid derivatives; meanwhile, the materials have excellent selective enrichment performance on the glycopeptide and separating capacity on a carbohydrate chain structure, have very high glycopeptide recovery rate and repeatability and have the wide application prospect in the fields of posttranslational modification proteomics research and the like.
Owner:WUHAN UNIV OF TECH +1
Polygonatum kingianum homogeneous polysaccharide and preparation method and application thereof
ActiveCN112062872ASignificant antioxidant effectEasy to prepareOrganic active ingredientsAntinoxious agentsCelluloseSugar
The invention discloses polygonatum kingianum homogeneous polysaccharide and a preparation method and application thereof, the total sugar content of the polygonatum kingianum homogeneous polysaccharide is more than 95%, and the molecular weight of the homogeneous polysaccharide is 1-70kDa. The method for preparing the polygonatum kingianum homogeneous polysaccharide comprises the following steps:taking dried polygonatum kingianum, performing degreasing, extracting, precipitating and drying to obtain a polygonatum kingianum polysaccharide crude product; dissolving the obtained polygonatum sibiricum polysaccharide crude product with distilled water, loading the solution to an anionic cellulose chromatographic column, and performing eluting with distilled water to obtain polygonatum sibiricum washing component polysaccharide; purifying the obtained washing component polysaccharide by using a gel chromatographic column, and performing eluting by using a NaCl solution to obtain the polygonatum kingianum homogeneous polysaccharide. The polygonatum kingianum homogeneous polysaccharide is applied to an in-vitro antioxidant process. According to the invention, polygonatum sibiricum homogeneous polysaccharide is extracted and separated from polygonatum sibiricum for the first time and is subjected to carbohydrate chain sequencing. The preparation method is simple, high-purity homogeneous polysaccharide can be rapidly obtained, and the preparation method is suitable for large-scale production.
Owner:YANGZHOU UNIV
Method of measuring glycosyl in protein
ActiveCN103134874AComplete structureGood repeatabilityComponent separationBiological material analysisLiquid ChangeFluorescence
The invention relates to a method of measuring glycosyl in protein. The method specifically comprises that after being processed in a liquid-changing mode, the protein is cut by reducing ferment; a carbohydrate chain is directly cut by added carbohydrate-cutting enzyme; after the obtained carbohydrate chain is processed in a purifying mode, fluorescence derivation is conducted, and excessive markers are eliminated; and then the content of obtained carbohydrate chain is measured. The method of measuring the glycosyl in the protein can well guarantee that the carbohydrate is fully cut, and the carbohydrate chain is complete in structure and good in repeatability, and furthermore is simple and easy to operate.
Owner:北京康弘生物医药有限公司
Analysis method of target board derivatization and MALDI-TOF-MS of reducible carbohydrate chain
ActiveCN107271593AHigh detection sensitivityImprove ionization efficiencyComponent separationGirard reagentRoom temperature
The invention belongs to the technical field of glycobiology, and particularly relates a method of the target board derivatization of a reducible carbohydrate chain. The method comprises the following steps: preparing a reducible carbohydrate solution, a Girard reagent T solution and a matrix DHB solution separately, and applying the matrix DHB solution, the reducible carbohydrate solution and the Girard reagent T solution at the same point of an MALDI-TOF-MS instrument target board separately; placing the target board subjected to sample application at room temperature till a sample point is completely dried. The invention further provides an analysis method of the MALDI-TOF-MS of a sample subjected to derivatization. The method is high in derivatization efficiency, can effectively prevent the degradation of acid carbohydrate chains, can detect both neutral carbohydrate chains and acid carbohydrate chains in a positive ion mode without switching, and is high in universality and simple and more efficient.
Owner:西安泰乐星生物科技有限公司
Method for efficiently and mildly reducing ovalbumin allergenicity
ActiveCN107047925ALower sugar chainReduced epitopeProtein composition from eggsAnimal proteins working-upEpitopeAnimal science
The invention discloses a method for efficiently and mildly reducing ovalbumin allergenicity. By repeated freezing and thawing pretreatment, ovalbumin allergenic parts are exposed to molecule surfaces, and by combination of transglycosylase and trypsin for synergistic treatment, ovalbumin carbohydrate chains and epitopes can be reduced, and ovalbumin and egg food allergenicity can be remarkably reduced. Compared with other traditional methods for reducing egg allergenicity by synergy of high-temperature high-pressure physical methods and an enzymic method, the method has advantages that egg allergenicity elimination pertinence and effectiveness are improved remarkably while egg white nutrition and quality damages are reduced to the maximum extent, thereby being an efficient and mild egg allergenicity elimination method. The method has a prospect in application to industrial development of low-allergenicity egg products.
Owner:NORTHWEST A & F UNIV
Lectin chip for detecting carbohydrate chain markers based on blood serum and commonly based on protein in blood serum and saliva as well as kit and application of lectin chip
InactiveCN105675893ARapid determination of specific bindingBiological testingSaliva sampleSalivary Proteins
The invention provides a lectin chip for detecting carbohydrate chain markers based on protein in blood serum or saliva as well as a kit and application of the lectin chip, relates to the lectin chip for detecting the carbohydrate chain markers in blood serum or saliva, and particularly relates to the lectin chip for detecting the carbohydrate chain markers based on protein in blood serum and saliva as well as a kit and application of the lectin chip. The invention aims at providing a non-injury lectin chip for detecting the carbohydrate chain markers by identifying protein in saliva or blood serum and a method thereof. The lectin chip for detecting the carbohydrate chain markers based on serum carbohydrate binding protein, provided by the invention, comprises a testing lectin probe set, wherein the testing lectin probe set at least comprises PTL-I, PNA, AAL, LTL, STL, BS-I, PTL-II, SBA, ACA, UEA-I, MAL-I and PHA-E+L. The lectin chip can be used for rapidly detecting a specific carbohydrate protein chain structure in blood serum and saliva samples, and rapidly judging the specific combination of carbohydrate chains in the samples, so as to determine whether corresponding people have hepatitis or not and determine whether people are ACHBLF patients or not.
Owner:NORTHWEST UNIV
Filter membrane assisted separation and identification method for glycoprotein full O-linked carbohydrate chain in biological sample
InactiveCN102768132AWide applicabilityEasy to separatePreparing sample for investigationMaterial analysis by electric/magnetic meansChemical reactionCell separation
The invention provides a filter membrane assisted separation and identification method for glycoprotein full O-linked carbohydrate chain in a biological sample and solves the problems that reaction efficiency is low, and few types of carbohydrate chains are identifiable in the prior art. By molecule sieving effect of 8-12KD filter membranes, carbohydrate chains released from glycoprotein are separated from the same, O-linked carbohydrate chains of the glycoprotein on 8-12KD molecule sieves are released by beta-elimination reaction, the O-linked carbohydrate chains flow out by means of cleaning and repeated centrifuging, protein is reserved on the filter membranes, and further separation of the O-linked carbohydrate chains is achieved. The method is effective in carbohydrate chain separation, avoids byproducts brought in due to non-specific adsorption and chemical reaction, is high in reaction efficiency and has simple reaction steps.
Owner:NORTHWEST UNIV(CN)
Application of a method for obtaining and detecting monosaccharides by blood degradation in cancer detection
ActiveCN103969371BSuitable for testingEasy to operateComponent separationOncologyBiomarker (petroleum)
The invention belongs to the field of medicines, and relates to an application of a method for degrading all carbohydrate chains in the blood to be monosaccharide and detecting the monosaccharide in cancer detection. A sample in the method for degrading the blood to obtain the monosaccharide and detecting the monosaccharide is the blood, and the cancer includes lung cancer, gastric cancer, ovarian cancer, penis cancer, esophagus cancer, oral cancer, biliary duct cancer, breast cancer, adenocarcinoma perampullaire, rectal cancer and bladder cancer. The method has the characteristics of simplicity and easiness in operation steps, easiness in popularization, short detection time, low requirement on the instrument, low detection cost, less consumption of blood and the like. The result displays that based on the content of eight monosaccharides in the blood, not only can a normal person and a cancer patient be distinguished, but also different cancers can be distinguished. The method for simplifying glycomics knowledge and applying in the detection of the biomarker of blood is first created in the world.
Owner:OCEAN UNIV OF CHINA
Application of lectin group recognized carbohydrate chain in distinguishing mucinous cystic neoplasm from serous cystic neoplasm
The invention discloses application of lectin group recognized carbohydrate chains in distinguishing mucinous cystic neoplasm from serous cystic neoplasm. The lectin group recognized carbohydrate chains disclosed by the invention are glycosylated protein carbohydrate chains recognized by lectin groups consisting of WGA (trticum vulgaris agglutinin), BPL (bauhinia purpurea lectin), STL (solanum tuberosum (potato) lectin), DBA (dolchos biflorus agglutinin), PTL-I (psophocarpus tetragonolobus lectin I) and MAL-I (maackia amurensis lectin I). The carbohydrate chains are different in capsula pancreatic fluid of patients suffering from mucinous cystic neoplasm and serous cystic neoplasm, the contents of the carbohydrate chains recognized by STL, WGA, BPL and DBA in the capsula pancreatic fluid of patients suffering from MCN (mucinous cystic neoplasm) are remarkably higher than those in the capsula pancreatic fluid of patients suffering from SCN (serous cystic neoplasm), the contents of the carbohydrate chains recognized by PTL-I and MAL-I in the capsula pancreatic fluid of the patients suffering from MCN are remarkably lower than those in the capsula pancreatic fluid of the patients suffering from SCN, the sensitivity of the combination of WGA and BPL in distinguishing the patients suffering from SCN from the patients suffering from MCN is 0.714, and the specificity is 1. The results show that the lectin group can be used for distinguishing the patients from SCN from the patients suffering from MCN.
Owner:GENERAL HOSPITAL OF PLA
Composition for separating and detecting exotic protein of carbohydrate chain, kit, as well as method and application of composition
ActiveCN108761088AImprove separation efficiencyHigh separation precisionBiological testingChemistryCarbohydrate chains
The invention relates to a composition for separating and detecting an exotic protein of a carbohydrate chain, a kit, as well as a method and application of the composition. The diagnosis conditions of the tumor caused by the exotic protein of the carbohydrate chain and the other related diseases are indicated according to the method, and the method is highly sensitively, and is rapid, convenientand automatic.
Owner:BEIJING HOTGEN BIOTECH CO LTD +1
Method for multiply detecting carbohydrate chain structure of glycoprotein through antibody-assisted lectin liquid-phase suspension chip
The invention belongs to the technical field of biology and relates to a method for multiply detecting a carbohydrate chain structure of glycoprotein through an antibody-assisted lectin liquid-phase suspension chip. According to the method, lectins are coupled to magnetic beads marked with different dyes, the magnetic beads which are coupled with different lectins are identified through a Bioplex detection system, and different glycoforms on the same protein can be simultaneously detected by adding the magnetic beads which are coupled with different lectins and have different serial numbers into a reaction, so that the detection flux of the carbohydrate chain structure is remarkably improved. According to the method, the carbohydrate chain structure on the glycoprotein can be quickly and specifically detected with high flux. The method can be further applied to detection of a clinical biological marker and provides an efficient way for research on disease glycomics.
Owner:FUDAN UNIV
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