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Separation and purification method of lycium ruthenicum polysaccharide and five polysaccharides obtained through separation

A technology for the separation and purification of Lycium barbarum polysaccharides, which is applied in the field of separation and purification of Lycium barbarum polysaccharides and the five kinds of polysaccharides obtained by separation, can solve the problems of shallow research, almost blank structure research of Lycium barbarum polysaccharides, and low product purity, and achieve The effect of avoiding destruction

Active Publication Date: 2013-06-19
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on the extraction technology of Lycium barbarum polysaccharides is relatively shallow, limited to the extraction of crude polysaccharides by traditional methods. The crude polysaccharides extracted from Lycium barbarum contain a large amount of impurities such as macromolecular proteins, pigments, inorganic salts and other small molecules. The purity is low, and it is a mixture of various sugar components and protein pigments. At present, no pure product of Lycium barbarum polysaccharides has been prepared, and the structure research on Lycium barbarum polysaccharides is almost blank.

Method used

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  • Separation and purification method of lycium ruthenicum polysaccharide and five polysaccharides obtained through separation
  • Separation and purification method of lycium ruthenicum polysaccharide and five polysaccharides obtained through separation
  • Separation and purification method of lycium ruthenicum polysaccharide and five polysaccharides obtained through separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 A method for isolating and purifying polysaccharides from Lycium barbarum fruit, comprising the following steps:

[0036] (1) Crushing of raw materials: add 4 times the volume of water to the fruit of Lycium ruthenicum, and grind it to a uniform state with a tissue grinder.

[0037] (2) Ultrasonic extraction: Homogenize Lycium barbarum fruit and put it into ultrasonic extraction equipment, temperature is 70°C, ultrasonic frequency is 40KHz, power is 50W, extraction time is 2h, the extract is filtered, and the residue is reheated with 2 times the volume of water for ultrasonic extraction for 1h .

[0038] (3) Alcohol precipitation: After the extract was concentrated in vacuum at 40°C, the concentration was adjusted to 80% by adding ethanol, and the polysaccharide precipitate was filtered after standing for 12 hours.

[0039] (4) Deproteinization: After dissolving the polysaccharide precipitate with water, add trichloroacetic acid to a concentration of 3%, s...

Embodiment 2

[0059] According to the extraction method described in Example 1, the difference from Example 1 is that the temperature is 50°C, the ultrasonic frequency is 40KHz, the power is 50W, the extraction time is 2h, and the yield of crude polysaccharide (LRP) is 1.2%.

Embodiment 3

[0061] According to the extraction method described in Example 1, the difference from Example 1 is that the temperature is 70°C, the ultrasonic frequency is 80KHz, the power is 50W, the extraction time is 2h, and the yield of crude polysaccharide (LRP) is 0.9%.

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Abstract

The invention relates to a separation and purification method of a lycium ruthenicum polysaccharide and five polysaccharides obtained through separation. Lycium ruthenicum fruits serve as raw materials and a polysaccharide purified product is obtained through extraction by means of ultrasound heating, depoteinization by trichloroacetic acid, decoloration by hydrogen peroxide, diethyl-aminoethanol (DEAE) cellulose column chromatography, Sephadex G-100 column chromatography and the like. According to structure analysis, the lycium ruthenicum polysaccharide is arabinogalactan in multiple branches. The extraction process is performed under a relatively moderate condition and a carbohydrate chain portion on polysaccharide molecules is conserved completely. After purification, five different polysaccharide components are obtained. The five polysaccharides obtained through separation are clear in structure, controllable in quality and significant in the fields of food, cosmetic, health care products, medicines and the like.

Description

technical field [0001] The invention relates to a method for extracting and purifying polysaccharides from Lycium barbarum black fruit as raw materials and five kinds of polysaccharides obtained by separation Background technique [0002] Lycium ruthenicum Murr belongs to Lycium L. of Solanaceae. Perennial shrub with thorns, spherical berries, purple-black when mature, high photosynthetic efficiency and strong resistance to stress, is a unique wild plant in the desert area of ​​Northwest my country that needs to be developed urgently, distributed in northern Shanxi and Ningxia , Gansu, Qinghai, Xinjiang, Tibet and other provinces. The fruit of black wolfberry is sweet and juicy, rich in vitamins, organic acids and sugars, and is often eaten raw or squeezed to make beverages; black wolfberry also has certain medical and health functions. Tibetan medicine is used to treat heart fever and heart disease. , Irregular menstruation, menopause and other diseases. Polysaccharides w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杜昱光彭强许青松李曙光王仲孚王文霞
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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