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Polygonatum kingianum homogeneous polysaccharide and preparation method and application thereof

A Polygonatum polysaccharide and polysaccharide technology, applied in the field of Polygonatum application, can solve problems such as unclear structure and unclear mechanism research, and achieve the effect of simple preparation method

Active Publication Date: 2020-12-11
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its structure is not clear, and the mechanism research is unclear

Method used

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  • Polygonatum kingianum homogeneous polysaccharide and preparation method and application thereof
  • Polygonatum kingianum homogeneous polysaccharide and preparation method and application thereof
  • Polygonatum kingianum homogeneous polysaccharide and preparation method and application thereof

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preparation example Construction

[0028] The preparation method of the present invention comprises the following steps:

[0029] A. Take the dried rhizome of Rhizoma Polygonatum, degrease, extract, precipitate with ethanol, and dry to obtain the crude Polygonatum polysaccharide;

[0030] B, after dissolving the crude Polygonatum polysaccharide obtained in step A with distilled water, load an anionic cellulose chromatographic column, elute with distilled water, and obtain Polygonatum water-washed component polysaccharide;

[0031] C. Purify the Polygonatum water-washed component polysaccharide obtained in step B with a gel chromatographic column, and elute with NaCl solution to obtain Polygonatum homogeneous polysaccharide.

[0032] In the step A of the present invention, first, the dried Polygonatum is subjected to ethanol degreasing operation, and the degreased Polygonatum is subjected to hot water extraction and ethanol overnight precipitation operation.

[0033] Step A of the present invention is as follow...

Embodiment

[0038] Preparation and Identification of Polygonatum Homogeneous Polysaccharide

[0039] A. Take 2KG of dry sealwort, add 10L alkaline water (0.2M NaOH), extract 3 times under boiling conditions, neutralize the acid, then add 4 times the volume of ethanol for alcohol precipitation, filter for 24 hours, and collect the precipitate Dry to remove residual ethanol.

[0040] B. Take 10 g of the obtained crude polysaccharide, add 100 mL of distilled water to dissolve, load the sample on a chlorinated anion exchange resin DE-52 chromatographic column, use distilled water to elute, use the sulfuric acid-phenol method to detect the sugar content and place it in a microplate reader at 490nm Detect, obtain the elution curve, collect the sugar-containing solution, dry, dialyze with 8000Da dialysis bag for 24 hours, freeze-dry to obtain Polygonatum water-washed polysaccharide.

[0041] C. The polysaccharide obtained from Polygonatum water fraction was dissolved with 0.2 mol / L NaCl solutio...

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Abstract

The invention discloses polygonatum kingianum homogeneous polysaccharide and a preparation method and application thereof, the total sugar content of the polygonatum kingianum homogeneous polysaccharide is more than 95%, and the molecular weight of the homogeneous polysaccharide is 1-70kDa. The method for preparing the polygonatum kingianum homogeneous polysaccharide comprises the following steps:taking dried polygonatum kingianum, performing degreasing, extracting, precipitating and drying to obtain a polygonatum kingianum polysaccharide crude product; dissolving the obtained polygonatum sibiricum polysaccharide crude product with distilled water, loading the solution to an anionic cellulose chromatographic column, and performing eluting with distilled water to obtain polygonatum sibiricum washing component polysaccharide; purifying the obtained washing component polysaccharide by using a gel chromatographic column, and performing eluting by using a NaCl solution to obtain the polygonatum kingianum homogeneous polysaccharide. The polygonatum kingianum homogeneous polysaccharide is applied to an in-vitro antioxidant process. According to the invention, polygonatum sibiricum homogeneous polysaccharide is extracted and separated from polygonatum sibiricum for the first time and is subjected to carbohydrate chain sequencing. The preparation method is simple, high-purity homogeneous polysaccharide can be rapidly obtained, and the preparation method is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of Polygonatum application, in particular to Polygonatum homogeneous polysaccharide, its preparation method and application. Background technique [0002] Polygonatum sibiricum (Polygonatum sibiricum) is the dried rhizome of Polygonatum sibiricum, a traditional Chinese medicine in my country, which has the effects of invigorating qi and nourishing yin, strengthening the spleen, moistening the lungs and kidneys. There are many varieties of Polygonatum included in the Chinese Pharmacopoeia, mainly including Polygonatum, Polygonatum yunnanensis and Polygonatum multiflora. Also known as chicken claw ginseng, yellow chicken dish, chicken head sealwort, tiger ginger, pen tube dish. Polygonatum polysaccharide is the rhizome of Polygonatum dried, extracted with hot water and precipitated with ethanol to obtain Polygonatum crude polysaccharide. Polygonatum polysaccharide (PSP) has good biological activity, PSP can ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P35/00A61K31/715
CPCC08B37/006C08B37/0003A61K31/715A61P39/06A61P35/00
Inventor 李晓军张雪
Owner YANGZHOU UNIV
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