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Lectin test chip for saliva sample, and treatment method thereof

A processing method and lectin technology, which is applied in the field of lectin test chip and its processing, can solve the problems such as O-glycosylated sugar chain spectrum that have not yet appeared, and achieve the effects of strong test pertinence, efficient detection, and low production cost

Inactive Publication Date: 2013-10-02
NORTHWEST UNIV(CN)
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Problems solved by technology

[0007] However, at present, there are few studies on the sugar chain structure of salivary glycoproteins in patients with type 2 diabetes, and there is no detection method that can quickly detect the N-glycosylation and O-glycosylation sugar chain profiles of salivary whole proteins in diabetic patients.

Method used

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  • Lectin test chip for saliva sample, and treatment method thereof
  • Lectin test chip for saliva sample, and treatment method thereof
  • Lectin test chip for saliva sample, and treatment method thereof

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Embodiment Construction

[0037] The present invention considers the differences of different genders and age groups, divides three groups of young males, elderly males and elderly females, and uses the lectin chip technology to carry out a large number of experiments and analyzes respectively.

[0038] The previous experiments analyzed the results of lectin chips with loading gradients of 2μg, 4μg, 6μg and 8μg, and it was found that the signal value of 4μg loading was significantly higher than that of 2μg loading; The signal values ​​of each lectin were not significantly different when the amount of lectin was used, some of them were a little bit higher at 6 μg, and some were a little bit lower; the signal values ​​of 8 μg sample amount were basically higher than the signal value of 6 μg sample amount, and only a few Decrease in lectins. In order not to cause false positives caused by too saturated samples or false negatives caused by too little samples, we choose 4 μg as the loading amount of the exp...

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Abstract

The invention provides a lectin test chip for a saliva sample, wherein a production cost is low, test targeting is strong, inherent advantages of a lectin chip are combined, and rapid, simple and efficient detection can be performed so as to obtain intermediate result information (saliva whole protein N-glycosylation and O-glycosylation carbohydrate chain structure information) adopted as type 2 diabetes mellitus evaluation. The lectin test chip adopts an epoxidation film base, and is characterized in that at least one of lectins such as PTL-I, LCA and SBA is immobilized on the epoxidation film base in a spotting immobilization manner. According to the present invention, the preparation method and the treatment method of the lectin test chip are optimized so as to establish the optimal reaction system for production of whole protein N-glycosylation and O-glycosylation information for saliva sample detection, and provide convenience for rapid and efficient detection of saliva glycoprotein carbohydrate chain structures of type 2 diabetes mellitus patients.

Description

technical field [0001] The invention relates to a lectin test chip and a processing method thereof, which are specially aimed at saliva samples to obtain information as an intermediate result of type 2 diabetes assessment. Background technique [0002] Lectin is a kind of sugar-binding protein that is not of immune origin and does not have enzymatic activity. It can specifically recognize and bind to a specific sugar chain sequence in a monosaccharide or polysaccharide with a special structure. It exists in plants, animals and microorganisms, and more than 300 lectins have been discovered from nature so far. According to the type of sugar chains bound, lectins in nature can be divided into several types, that is, they can specifically bind to a certain sugar molecule, which is called the sugar lectin. In this way, lectins can generally be divided into six categories: D- Mannose or D-glucose lectins (such as concanavalin A); N-acetylglucosamine lectins (such as malt lectins)...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C40B40/10C40B50/18
Inventor 李铮范菲艳于汉杰吴昊翔
Owner NORTHWEST UNIV(CN)
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