Lectin microarray for detecting carbohydrate chain marker based on sialoprotein and detection method of carbohydrate chain marker using same

Abstract

The invention relates to a lectin microarray for detecting a carbohydrate chain marker based on sialoprotein and a detection method of the carbohydrate chain marker using the same. The lectin microarray can nondestructively detect the carbohydrate chain marker by distinguishing sialoprotein. The lectin microarray comprises a testing lectin probe group, wherein the testing lectin probe group comprises a vicia villosa lectin, an aleuria aurantia lectin, a pea lectin, a winged bean lectin-II an a winged bean lectin-I, and the winged bean lectin-II and the winged bean lectin-I are used as internal reference lectins. The lectin microarray has the advantages that the human body is not injured when the carbohydrate chain marker for detecting the carbohydrate chain marker is harmless to a human body, less samples are used, the detection speed is high and the sensitivity is high, and the lectin microarray can be applied to identification of stomach cancer.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a protein-based lectin chip for detecting sugar chain markers and a method thereof. Background technique [0002] Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, especially in East Asia, Eastern Europe, and South America due to the lack of significant early features. Currently commonly used diagnostic markers for gastric cancer include: CEA, CA19-9, CA72-4, CA125, AFP, etc., but all have limitations. Histopathological examination is still the gold standard for the diagnosis of gastric cancer at present. However, in view of the inherent defects of histological examination, such as destructive examination, dynamic detection, and sampling differences, it is important to seek non-invasive examination for early diagnosis of gastric cancer. The key to gastric cancer. [0003] With the progress of society and the development of ...

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Problems solved by technology

[0006] For example, some functional sugar chains have been found in the research of gastric cancer, and the representative one is the up-regulated expression of N-acetylglucosaminyltransferase V (GnT-V) in gastric cancer cells, which leads to the synthesis of β1,6-linked N-acetyl Glucosamine branched N-glycan (β1,6GlcNAcbranchedN-Glycan), this branched sugar chain affects the phosphorylation of tyrosine in catenin, resulting in the damage of β catenin and P120 protein, destroying the relationship between catenin and N-glycan -The stability of sugar chain binding affects the adhesion ability between cells and increases the ability of cell transfer and infection

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