41 results about "Biological half-life" patented technology

A polyanionic polymer can improve the bioactivity and water-solubility properties of a drug to which it is joined. The inventive method provides a monodispersed preparation of a recombinantly-produced polyanionic polymer that can be easily manipulated, such as lengthened. An active moiety may be chemically or recombinantly joined to a polyanionic polymer to increase its biological half-life and/or solubility. The instant invention also provides a method for targeting the delivery of a polyanionic polymer conjugate or fusion protein to a specific cell type or tissue.

The present invention discloses antitumor drug PEGylation and applications of the antitumor drug PEGylation in reversal of tumor multidrug resistance. The PEGylated antitumor drug is prepared by conjugating activated PEG onto an antitumor drug, and can be used for preparing the drug for reversal of tumor multidrug resistance. According to the present invention, the antitumor drugs (particularly water insoluble drugs) containing amino, carboxyl, hydroxyl and other modifiable groups are PEGylated so as to increase the water solubility, such that excellent characteristics of micelle self-assembly forming, production of invisible nanoparticles or active targeting tumor nanoparticles, drug release behavior regulation, system toxicity reducing, biological half-life improving, EPR effect production, tumor passive/active targeting production, tumor multidrug resistance reversal and the like are provided, and good clinical application prospects are provided.

The invention relates to polypeptide conjugates comprising a polypeptide exhibiting G-CSF activity and having an amino acid sequence that differs from the amino acid sequence of human G-CSF in at least one specified introduced and/or removed amino acid residue comprising an attachment group for a non-polypeptide moiety, and having at least one non-polypeptide moiety attached to an attachment group of the polypeptide. The attachment group may e.g. be a lysine, cysteine, aspartic acid or glutamic acid residue or a glycosylation site, and the non-polypeptide moiety may e.g. be a polymer such as polyethylene glycol or an oligosaccharide. The conjugate, which has a reduced in vitro bioactivity compared to hG-CSF, has one or more improved properties such as increased biological half-life and increased stimulation of neutrophils.

The invention relates to a method for synthesizing mono pegylation-thymopentin (mPEG-TP5) by solid phase and liquid phase combination. The method comprises the following steps of: connecting modified polyethylene glycol (mPEG-SCM) to side chain amino of lysine protected by amino by adopting liquid-phase synthesis technology; synthesizing mPEG-TP5 by adopting solid-phase synthesis technology; analyzing and purifying high-efficiency liquid phase through dialysis; and identifying the product structure through nuclear magnetism and mass spectrum. The mono pegylation-thymopentin synthesized by themethod improves the defect that the conventional parent medicament thymopentin has quick biodegradation, poor digesting stability, short biological half-life and the like, and has good application prospect.

Pharmaceutical compositions comprising an extended release formulation of active compounds effective in the treatment of various pathological conditions are provided. More particularly, the invention provides methods of making and using extended release formulations comprising active compounds that present formulation challenges such as short biological half-life, instability, highly water soluble and/or high dose requirements. Specifically, orally administrable extended release pellet and tablet formulations of isovaleramide are preferred.

The invention discloses a preparation method and application of a triptolide-carboxylation chitosan coupling drug. The preparation method comprises the following steps: (1) synthesis of intermediate carboxylation triptolide (TP-L); and (2) synthesis of the triptolide-carboxylation chitosan coupling drug (TP-L-CChit). The triptolide-carboxylation chitosan coupling drug (TP-L-CChit) prepared by themethod, as a pro-drug, has application for preparing drugs for treating rheumatoid arthritis, tumors and Alzheimer's disease; the drug is simple in method, easy for production preparation, high in yield and rich and easily obtained in raw materials, the product is high in water solubility, long in biological half life period, high in availability, free of toxic or side effect and good in curativeeffect and is innovation for preparing drugs for treating rheumatoid arthritis, tumors and Alzheimer's disease, and the economic and social benefits are obvious.

The invention relates to a preparation method and an application of a galactosyl proserum fused interferon, belonging to the targeted biological medicine technical field. The invention takes a proserum fused interferon as a galactosyl precursor, can provide enough sites for glycosylation, and then more effectively prepare the galactosyl proserum fused interferon with different sugar densities (between 7 and 35). Compared with the proserum fused interferon, the compound has obvious liver targeting character and the same pharmacological activity, is hopeful to be used for preparing a novel virus hepatitis targeted therapeutic medicine, obviously improves the medicine concentration of IFN in the liver, prolongs the biological half-life of the galactosyl proserum fused interferon, effectively reduces the dosage of the clinic IFN, eases the pain of patients due to repeated injection, reduces generation of side reaction, greatly reduces the treatment cost of the patients, improves the treatment level of virus hepatitis in China, promotes excellent health of Chinese people, and has good social benefit and significant economic benefit.

The invention relates to a long-acting exenatide derivative and belongs to the technical field of polypeptide compounds. The exenatide derivative long in pharmacologic action time is prepared by performing structural optimization on a exenatide sequence to allow the exenatide to have effects of lowering sugar and reducing weight, conjugating double-dose exenatide with single-dose fatty acid chain,and using the fatty acid chain to bring the serum albumin binding effect into play. The invention further discloses the preparation method of the exenatide derivative, the pharmaceutically acceptablesalt of the exenatide derivative, an exenatide derivative drug, a pharmaceutical composition and application of the exenatide derivative in the preparation of drugs for treating and/or preventing diabetes, obesity, hyperlipidemia and non-alcoholic fatty liver disease. The long-acting exenatide derivative can have a weight reducing effect on the basis that the sugar lowering activity of the exenatide derivative is kept, the biological half-life of the exenatide derivative is evidently prolonged as compared with the exenatide prototype, the biological half-life of part of the exenatide derivatives reaches more than 36 hours, and the sugar-lowering and weight-reducing action time is prolonged greatly.

The invention relates to ultrafine powder of a macrolide drug and a preparation method for the ultrafine powder. The macrolide drugs are widely applied in clinic with definite curative effects. The drugs are wide in antibacterial spectrum, strong in antibacterial activity in vivo and long in biological half life period, and have good clinical use value. A method for preparing the ultrafine powder of the macrolide drug comprises: in a homogeneous solution containing the macrolide drug, applying ultrasound with frequency of 10kHz to 500kHz, power of 1mW to 5000W and acoustic intensity of 0.1mW/cm2 to 500W/cm2 to quickly obtain crystals of the macrolide drug; and then directly obtaining the ultrafine powder of the macrolide drug through conventional operations such as solid collection, washing and drying and the like. The ultrafine powder prepared according to the method provided by the invention does not contain a substrate, and has the advantages of high drug loading capacity, high dissolving speed and proneness to achieve better bioavailability, stability and safety, thereby meeting the requirements on bioavailability, dosage reduction and adverse effects of drugs, and having broad clinic application prospects.

The spray for treating burns, scalds and chronic ulcer has PEG-aFGF decorated chemically with polyethylene glycol as acid fibroblast growth factor as main medicinal component. The spray contains mainly PEG-aFGF decorated chemically with polyethylene glycol as acid fibroblast growth factor 1-100 microgram, hot zedoary oil 1-100 mg, protecting agent 0.001-0.05 mg, preservative 0.001-0.01 mg, stabilizer 0.0001-0.001 mg and corrective 0.01-0.2 mg in each milliliter, and the rest is physiological saline or water for injection. The spray has high stability, long biological half time, high safety and other features.

The invention discloses a preparation method of anti-acne cream and belongs to the technical field of skin-care products. An ionic liquid is made with 1,1,3,3-tetramethylguanidine and is used as an extracting solvent; under cooperation with ultrasonic assistance, the extracting solvent penetrates in the cells of medicinal materials, effective ingredients are dissolved in the extracting solvent, and the effective ingredients of traditional Chinese medicine are protected; purslane herb is added to resist bacteria and diminish inflammation; Buchu helps control sebum secretion, fight senility, andrelieve pore blocking due to sebum; Mongolian milkvetch root and otherness are added to promote blood circulation, heal up sores and promote granulation; Chinese angelica root helps improve skin whiteness and transparency; phosphate materials made with VC can provide local whitening and acne fading; chitosan is degraded into chitooligosaccharide, and catalytic oxidization is carried out via laccase; synergy between carboxyl groups newly generated and original amino groups on C2 provides antioxidant protection for VC, keeps water of the smeared skin, maintains moisture and maintains water-sebum balance; active enzymes herein promote skin metabolism, accelerate acne healing, extend biological half-life and improve skin absorption rate.

The invention relates to long-acting GLP-1 and a preparation method and application thereof, relates to the field of type 2 diabetes mellitus medicines, and solves the problems that GLP-1 is short inself biological half-life, poor in effect of reducing blood sugar, and difficult in exertion of effective treatment effects. The preparation method of the long-acting GLP-1 comprise the step that human umbilical cord mesenchymal stem cells are disinfected with recombinant adenovirus AdV-GLP-1 of 30MOI for 48h or above, so that the long-acting GLP-1 is obtained, and the infection efficiency is 90%or above. The invention relates to an application of the long-acting GLP-1 to preparation of the medicines for treating type 2 diabetes mellitus. The long-acting GLP-1 is used for modifying the humanumbilical cord mesenchymal stem cells, so that the long-acting GLP-1 having high blood sugar reducing activity and long pharmacological action time is obtained. The prepared long-acting GLP-1 is injected to mice suffering from the type 2 diabetes mellitus, and the long-acting GLP-1 achieves long-acting treatment effect in bodies of the mice.