The invention relates to an experimental method measuring
soil respiration via laboratory simulating dry-wet alternate responses. The experimental method at least comprises the following steps: step 1, selecting a wide-mouthed
bottle, recording the weight of the wide-mouthed
bottle, filling the wide-mouthed
bottle with air-dried soil, recording the total weight of the wide-mouthed bottle and the air-dried soil, hanging a
desiccant bag in the wide-mouthed bottle, and placing the wide-mouthed bottle in a
temperature control system, wherein the wide-mouthed bottle comprises a perforated rubber plug and a
glass tube, the perforated rubber plug is arranged at the opening of the wide-mouthed bottle, the
glass tube is arranged in the hole of the perforated rubber plug, the top end of the
glass tube is sleeved by a latex tube, and the top end of the latex tube is blocked by a flatjaw pinchcock; step 2, measuring gas which is exhausted from
soil respiration according an alkali absorption method and an acid-base
titration method. The experimental method has the excellent effects that the values of
soil respiration within the specified time in the design of different experiments can be achieved, and samples of different numbers can be measured; the operation is simple, convenient and rapid, and the production cost is low; the experimental method can be operated in a common laboratory.