42 results about "A-type proanthocyanidin" patented technology

A protocyanidin sloow release microcapsule has microcapsule size smaller than 3 mm, protocyanidin content of 0.25-25 wt% and sustained release period of 12-48 hr in buffering phosphoric acid solutionof pH 7.4. The wall of the microcapsule is one three layer structure including agglomerate calcium alginate layer, chitosan and alginate complex layer and deposited chitosan layer. The microcapsule is prepared through compounding water solution of calcium alginate containing protocyanidin, preparing chitosan-CaCl2 solution, adding miniature liquid drop of water solution of calcium alginate containing protocyanidin into chitosan-CaCl2, solution and subsequent processing. The release rate of the coated protocyanidin is adjustable.

The invention discloses a preparation method of proanthocyanidin. In the method, grape seed, pine bark, purple potato or any other plant with high proanthocyanidin content is used as a raw material, an ethanol aqueous solution is used as an extraction solvent, and a double water phase extraction method is utilized to efficiently prepare proanthocyanidin from a crude proanthocyanidin extract. The invention has the advantages of high product yield, high product purity, low solvent consumption, low cost and the like, and can easily implement industrial production.

The invention discloses a method for preparing proanthocyanidins liposomes, and relates to a method for preparing liposomes. The method solves the problems of large grain size and difficult absorption of a human body by protecting proanthocyanidins in a microcapsule form in the prior art. The method comprises the following steps of: 1, putting soya bean lecithin and cholesterol in a pear-shape flask, adding mixed solution of diethyl ether and absolute ethyl alcohol, adding proanthocyanidins and phosphate buffer, and mixing to obtain mixed solution; 2, performing ultrasonic processing on the mixed solution in a 25 DEG C water bath, performing rotary evaporation to form a film in a water bath at the temperature of between 30 and 50 DEG C and under the pressure of between 400 and 700MPa, adding phosphate buffer again, and performing hydration to obtain coarse suspension; and 3, and filtering the coarse suspension by using a filter membrane of 0.45 mu m. The liposomes are adopted for encapsulating the proanthocyanidins, so the proanthocyanidins are more stable, have small grain size and target, and are favorable for absorption of the human body; the encapsulation rate of the proanthocyanidins liposomes is 88.89 percent; and the preparation process is simple.

The invention relates to a proanthocyanidin effervescent tablet and a method for preparing the same. The effervescent tablet consists of the following components in the following weight percentage: 2 to 15 percent of proanthocyanidin, 1 to 10 percent of vitamin C, 10 to 40 percent of beta-cyclodextrin, 20 to 50 percent of citric acid, 30 to 35 percent of sodium bicarbonate, 0.5 to 1 percent of protein sugar, 0.1 to 2 percent of flavoring essence, and 0.05 to 0.1 percent of magnesium stearate. The proanthocyanidin effervescent tablet has the advantages that the proanthocyanidin effervescent tablet can be conveniently carried around, used and absorbed as the common effervescent tablets, and simultaneously has characteristics of high concentration of functional substances and strong functionality, as well as bright color of the proanthocyanidin; the proanthocyanidin effervescent tablet has the greatest advantage that the stability of the proanthocyanidin is greatly improved after the proanthocyanidin is embedded by the beta-cyclodextrin; the vitamin C is added in the effervescent tablet to further prevent the proanthocyanidin from being oxidized and act synergistic effect on the antioxidant activity of the proanthocyanidin so as to improve the functionality thereof; furthermore, the proanthocyanidin effervescent tablet has simple formulation, easy preparation, and low cost.

The present invention relates to a procyanidins health beer production method using leftover grape seeds of a wine plant for brewing. The procyanidins health beer production method is as follows: crushing of a raw material of the leftover grape seeds of the wine plant, addition of grape seed powder in boiling stage and yeast-rising stage, and determination of procyanidins in health beer and determination of DPPH free radical removal capability of the procyanidins health beer. The procyanidins health beer production method has the advantages of simple process, low cost and easy industrial production, and the like, and the obtained procyanidins health beer has high procyanidins content, and has strong free radical scavenging capability and antioxidant health-care effect.

The method of extracting natural dye from plane tree is to extract natural active dye from bark of plane tree containing resorin A type proanthocyanidin in 22-27 % possessing dyeing activity in the molecular weight range of 350-2000. By means of extracting, adsorbing, refining and other technological steps, coffee dye and pale yellow dye of purity over 90 % are prepared. The obtained dyes have high dyeing performance and high fastness on hair, wool and fabric, and will have raised use effect when being compounded with hydrolyzed tannin or flavone glycoside dye. The obtained dyes are used in dyeing hair, wool, fabric, etc.

The invention discloses a procyanidin capsules and a process for manufacturing the same. The capsules are manufactured by taking procyanidin and astragalus polysaccharide as main materials and caseinphospho peptides or D-mannitol, magnesium stearate and gelatin as auxiliary materials. The capsule has the advantages of being conveniently taken, avoiding absorbing moisture and having functions of immunoregulation, anti-ageing and radiation hardening, protecting cardiovascular health by removing free radicals in vivo, and preventing crosslinking of skin collagens, inhibiting pigmentation, dispelling wrinkles and whitening the skin, keeping skin elasticity and delaying ageing.

The invention relates to proanthocyanidin sustained release nano-micro spheres. The proanthocyanidin sustained release nano-micro spheres comprise 1-5 parts of proanthocyanidin, 1-12 parts of chitosan or derivatives thereof, 1-12 parts of phospholipid or derivatives thereof and 1-10 parts of excipients, wherein the chitosan and the derivatives thereof are one or more of high, medium and low-molecular weight chitosan, chitosan oligosaccharide, chitosan quaternary ammonium salt and carboxymethyl chitosan. The particle size of the proanthocyanidin sustained release nano-micro spheres is 10nm-0.3 mu m; the proanthocyanidin is prepared into the powder sustained release nano-micro spheres, and the proanthocyanidin sustained release nano-micro spheres can directly play a role in the lung as a pulmonary inhalation medicine-carrying formulation, improve the bioavailability of the proanthocyanidin and reduce the dosage by sustained release; the proanthocyanidin nano-micro spheres can also be applied as an oral sustained release formulation; the proanthocyanidin nano-micro spheres have appropriate particle size and morphology and other powder properties, as well as sustained release; furthermore, the preparation method has the advantages of simplicity and convenience in operation, stable process and easiness in industrial production.

The invention discloses procyanidine-modified carboxymethyl chitosan, a preparation method thereof, and application thereof in the fields of medical materials, health-care foods, cosmetics and the like. The procyanidine-modified carboxymethyl chitosan keeps the characteristics of carboxymethyl chitosan and has high oxidation resistance, antibacterial activity, barrier properties and the like, and is worthy of being applied.

The invention provides a procyanidine soft capsule, which comprises contents consisting of procyanidine, plant oil, beewax and phospholipid according to mass percentage: 10 percent to 60 percent of procyanidine mixtures, 35 percent to 87.5 percent of plant oil, 0.5 percent to 2.5 percent of beewax and 1 percent to 5 percent of phospholipid. The procyanidine soft capsule can be prepared from the procyanidine mixture, the plant oil, the beewax, the phospholipid and other human body acceptable medical auxiliary ingredients through a pressing method. The procyanidine soft capsule provided by the invention has the advantages that the production cost is low, and the production process is simple, convenient and advanced. The appearance of the procyanidine soft capsule is attractive, the carrying is convenient, the dosage is small, one capsule is taken in one day, and the taking compliance is good. The procyanidine soft capsule is particularly suitable for middle-aged people and old people and has good anti-oxidation health-care effects.

The invention discloses a preparation method of a procyanidin molecular imprinting sensor. The preparation method comprises modifying a glassy carbon electrode with graphene oxide and chloroplatinic acid, adding 56 to 66% by mass of deionized water, 6 to 10% by mass of hydroxyethyl methacrylate, 6 to 10% by mass of methylene succinic acid, 12 to 20% by mass of 1-allyl-3-butylimidazolium hexafluorophosphate, 1.0 to 4.0% by mass of dimethyl azobisisobutyrate and 0.5 to 2.0% by mass of procyanidin into a reactor, stirring the materials in an oxygen-free atmosphere for a reaction for 6-8h, removing a template molecule through a mixed solution of methanol and acetic acid, carrying out drying to obtain a procyanidin molecular imprinting polymer, and dispensing the procyanidin molecular imprinting polymer to an electrode modified by graphene oxide and chloroplatinic acid to obtain the procyanidin molecular imprinting sensor. The procyanidin molecular imprinting sensor has high affinity and selectivity, has high sensitivity, good specificity and a rapid detection rate and can be recycled.

The invention discloses a method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application, and the human liver cancer cell HepG2 cell is used for experiment for determination of effects on HepG2 cell proliferation, dose effect and time effect of induction of HepG2 cell autophagic death of proanthocyanidins, content of intracellular ROS (reactive oxygen species and high active oxygen radicals), cell cycle distribution and mitochondrial membrane potential. Results confirm that the proanthocyanidins can induce human liver cancer cell HepG2 cell autophagic death by stimulating of the ROS pathway, and the results are applied to the prevention and treatment of liver cancer.

The present invention discloses an extraction method of original anthocyanins. In the method, the mulberry fruit is used as raw material; the mulberry after being cleaned is crushed into fruit puree; the fruit puree is stirred evenly. After dehydration and doffing of sugar, the fruit puree is dried at a temperature of minus 20 DEG C until the content of water is lower than 5 percent; the dried fruit puree is crushed in a super-micro way. Then the known extraction method is used for extracting. The present invention has a high production rate; no solvent residue or environmental pollution can be produced; the comprehensive value of utilization is also high.

The invention relates to a proanthocyanidin derivative which is prepared by carrying out acetylation reaction on proanthocyanidin extracted from grape seeds and acetic anhydride. The preparation method comprises the following steps: pulverizing fresh grape seeds, degreasing, adding a 60% ethanol water solution as an extractant, carrying out vibrating extraction in an ultrasonic extracter for 20 minutes, repeating the extraction process three times, concentrating the volume, carrying out low-temperature centrifugal filtration, extracting with ethyl acetate three times, distilling the extract with a rotary evaporator, concentrating to obtain reddish brown proanthocyanidin, and carrying out acetylation reaction with acetic anhydride to obtain the acetyl proanthocyanidin;Or extracting at 50 DEG C four times (1 hour a time) by using a 60% ethanol water solution as an extractant, concentrating the extract, carrying out low-temperature centrifugal filtration, extracting with twice of ethyl acetate, and carrying out acetylation reaction with acetic anhydride to obtain the acetyl proanthocyanidin. The proanthocyanidin derivative is used for preparing health foods and medicines for preventing and treating tumors.

The invention discloses medicinal application of A type proanthocyanidin trimer polyphenol. The medicinal application is that the A type proanthocyanidin trimer polyphenol is taken as one of active compositions or the only one active composition to prepare medicine preparations for treating immunologic diseases. Experiments show that the half cell lethal concentration of the A type proanthocyanidin trimer polyphenol is larger than 50 mu g / mL, and the A type proanthocyanidin trimer polyphenol has inhibiting effect on propagation of lymphocyte induced by mitogen ConA and secretion of cytokines IL-6 and IFN-gamma important for immunoloregulation and inflammation reactions, is expected to be taken as one of active compositions or the only one active composition for preparing medicine preparations for treating immunologic diseases, especially is expected to be taken as one of active compositions or the only one active composition for preparing medicine preparations for treating rheumatoid arthritis, and has medicinal value.

The invention relates to the technical field of daily cosmetics, in particular to a procyanidine white carbon black sunscreen which comprises procyanidine, cyanidine, white carbon black, olive oil, hoba oil, dimethicone, glycerin, vitamin, stearic acid, pyrrolidone carboxylic acid sodium, essence and deionized water. Compared with sunscreen products of the prior art, the procyanidine white carbon black sunscreen has higher ultraviolet absorbing and reflecting capability and stability and better whitening effect and moisturizing effect and can converge and tighten loose skin, reduce wrinkles and enable the skin to be exquisite, and utilization values of fumed white carbon black and raw materials containing procyanidine / cyanidine are increased.

The invention discloses an analysis method for metabolin of A-type proanthocyanidins, which comprises the steps of collection of a biological sample, preparation of metabolin solution, detection of the metabolin and the like, wherein the preparation of the mtabolin solution comprises preparation of hydrolysate, preparation of crude extraction of the metabolin and purification of the metabolin crude extraction. The analysis method for the metabolin of the A-type proanthocyanidins is good in separating purification effect, efficient, fast, high in result accuracy and good in reproducibility, and not only facilitates understanding of a metabolic mechanism and the metabolin, but also plays an important role in further utilization of the A-type proanthocyanidins.

The invention discloses an application of plant source proanthocyanidins for improving intestine development and meat quality of broilers. The application is characterized in that the proanthocyanidins are selected from a grape seed extract, and are waste natural plant resources enriched in production technologies of brewing grape wine and the like in factories. Two months before the broilers appear on the market, according to the proportion of a basic ration, 400mg / kg of the plant source proanthocyanidins are added continuously each day, uniform stirring is performed, and the broilers are fedaccording to the quantity on time. According to the method, in the implementation process, the proanthocyanidins are good in palatability and the broilers like to eat the proanthocyanidins. Besides,the proanthocyanidins can promote the development of intestines of the broilers, the meat quality can be improved, the immunity of the broilers can be further improved, the attack rate is reduced, andthe consumption of drugs of antibiotics and the like is reduced. Therefore, the produced broilers are green foods, are delicious in meat quality and good in mouth feel, and have high economic value and social significance.

The invention discloses application of proanthocyanidin to preparation of a functional food or medicine for improving the sperm quality of obese men and also discloses application of a pharmaceuticalcomposition taking the proanthocyanidin as an active component, and a pharmaceutically acceptable additive to preparation of the functional food or medicine for improving the sperm quality of the obese men. A research proves that the proanthocyanidin has an obvious reduction effect on a weight gain rate of rats fed by high fat diet, and has the effects of improving the level of serum testosterone,protecting the structural integrity of testicles, improving the spermatogenesis obstruction caused by obesity, reducing excessive apoptosis of spermatogenesis cells of the testicles, improving the quantity and vitality of sperms and reducing the distortion rate of the sperms, so that the provided pharmaceutical can be used for improving the sperm quality of the obese men and protecting a reproductive function of the obese men. The invention digs novel medical application of the proanthocyanidin and provides meaningful reference for solving the reproductive dysfunction of the obese men.

The invention relates to compositions, such as pharmaceuticals, foods, food additives, or dietary supplements, containing a flavanol, an A-type procyanidins, a B-type procyanidin or a derivative thereof, and methods of use thereof, for treatment and / or prevention of occlusive thrombosis and related conditions.

The invention provides a proanthocyanidin oligosaccharide powder for endocrine regulation and a preparation method of the powder. The powder comprises the following components in parts by weight: 3-6parts of plant extracts with proanthocyanidins of greater than or equal to 95%, 70-90 parts of inulin, 10-20 parts of MCT powder, 1-2 parts of coffee powder, 0.5-1.5 parts of tea extracts with tea polyphenols of greater than or equal to 95%, and 0.5-1.5 parts of water-soluble pearl powder. According to the present invention, proanthocyanidins are combined with inulin, MCT powder, coffee powder, tea polyphenols and water-soluble pearl powder to obtain the proanthocyanidin oligosaccharide powder for endocrine regulation, and the powder not only has a good synergistic effect of regulating digestion and absorption, and increases the absorption utilization rate, but also is conducive to the antioxidant ability of proanthocyanidins, tea polyphenols, etc, in the body, improves the ability of removing free radicals in the body, has a more significant conditioning effect, is convenient for taking, and is effectively used in the powder health care products for treating facial skin diseases caused by pigmented dermatosis and skin glandular secretion disorders.

The method relates to the fields of plant extraction and healthcare foods, and especially relates to a method for extracting and purifying procyanidine from grape seed with macroporous absorption resin. The method comprises the specific processes of resin pretreatment, grape seed extract liquid preparation, and grape seed extract liquid purification. The grape seed extract liquid purification process comprises macroporous resin purification and chromatography resin purification. With the method, a finished product requirement of high purity can be achieved with the two-stage purification operation; in all processes, a basic concept of procyanidine high-purity product dependence on organic solvent is avoided to a maximal extent, and the extract liquid is completely purified with resin, such that residue caused by the application of organic solvent is avoided. The invention provides the green and environment-friendly procyanidine high-purity extraction method which is suitable for actual productions.

The invention discloses a procyanidine extraction method. The extraction method comprises the following steps: 1) taking washed and dried grape seeds and grape skin, and uniformly mixing the grape seeds and grape skin according to the proportion of 12-15:2-6, fragmenting the mixture to powder; 2) adding powder in the step 1) in a mixing extract, wherein the mixing extract is formed by uniformly mixing ethanol, ethyl acetate and water according to volume ratio of 12-16: 6-9: 3-5; and uniformly stirring the materials; and 3) performing ultrasonic extraction on the mixture in the step 2) for 30-50 min at condition of 8-10 MPa, 3-3.5 MHz ultrasonic wave and at 15-22 DEG C, quickly cooling the material to room temperature, filtering the material, and performing vacuum drying on a filtrate. The method employs waste material grape seed and grape skin which cannot be directly eaten, the waste material grape seed and grape skin are taken as the preparation raw materials of procyanidine, the waste can be used, cost is saved, under effect of a mixed solvent, the procyanidine can be dissolved, and is extracted under high pressure and low temperature through ultrasonic wave, the procyanidine is difficulty oxidized, the extraction efficiency is high, impurity is little, and the speed is fast.

The invention discloses a procyanidin nanoemulsion drugs the composition of which is procyanidin, non-ionic surfactant, oil, deionized water, its preparation method is surfactant is loaded and weighed according to the proportion formula, choosing oil phase, and make sure that the HLB for its emulsion is close to the HLB of surfactant, then well prepared surfactants and oil phase is mixed according to the original proportion formula, adding proportional anthocyanin, blending and stirring completely, slowly dropwising deionized water and stirring at room temperature until it has similar viscosity with water and become clear transparent solution which is procyanidin nanoemulsion drug. Nano-procyanidin significantly improves the solubility, dispersion of procyanidins in water and its bioavailability, improves the stability of procyanidins. The nano-emulsion can be widely used in cosmetics, pharmaceuticals, beverages, health care products, fodder and other fields.

The invention discloses a preparation method of a procyanidine facial mask. The facial mask comprises the following main components in parts by weight: 1-5 parts of grape skins, 2-5 parts of aloe juice, 2-5 parts of celery juice, 2-5 parts of towel gourd juice, 2-5 parts of pearl powder, 4-10 parts of milk, 1-3 parts of honey, 1-2 parts of lemon juice, 10-20 parts of glycerol, and 40-75 parts of distilled water. The preparation method of the procyanidine facial mask mainly comprises: obtaining raw materials, performing osmosis concentration through a reverse osmosis membrane on the raw materials, and performing emulsion treatment, facial mask paper soaking and packaging. The procyanidine facial mask disclosed by the invention is prepared from whole plant extracts, does not contain any additive, and has effects of moisturizing, whitening, anti-oxidation.

The invention provides a feeding method of proanthocyanidins on horses. The feeding method includes: adopting coarse feed and concentrated feed for mixed feeding, wherein the proanthocyanidins is mixed in the concentrated feed, and the feeding amount of the proanthocyanidins per kilogram of the body weight of the horses is 40-60mg / kg. The reasonable proanthocyanidins feeding method on the horses is provided for healthy culture of the horses, and daily health and competition demands of the horses are ensured.

The invention belongs to the field of healthcare product processing, and provides a pharmaceutical composition. The pharmaceutical composition comprises, by weight, 2.5-5% of procyanidine and 95-97.5%of traditional Chinese medicine extract. A method for preparing the pharmaceutical composition includes sequentially progressively increasingly adding the procyanidine with the low weight into the traditional Chinese medicine extract by the aid of an equal progressive increase process; sufficiently grinding the procyanidine and the traditional Chinese medicine extract until the procyanidine and the traditional Chinese medicine extract are uniform; sieving the procyanidine and the traditional Chinese medicine extract to obtain the pharmaceutical composition. The pharmaceutical composition hasthe advantages that beneficial effects of resisting oxidation and scavenging free radicals can be realized by the procyanidine, effects of benefiting qi, removing blood stasis, regulating menstruation, removing freckle and nourishing skin can be realized by traditional Chinese medicine compositions, and accordingly good auxiliary prevention and treatment effects for preventing and treating skin damage due to the free radicals, promoting blood circulation and enhancing skin cell development can be realized by the pharmaceutical composition; tablets, capsules, granules, oral liquid or wine can be prepared from the pharmaceutical composition according to conventional processes, and the pharmaceutical composition can be applied to preparing healthcare products or additives in cosmetics.

The invention relates to proanthocyanidin sustained release nano-micro spheres. The proanthocyanidin sustained release nano-micro spheres comprise 1-5 parts of proanthocyanidin, 1-12 parts of chitosan or derivatives thereof, 1-12 parts of phospholipid or derivatives thereof and 1-10 parts of excipients, wherein the chitosan and the derivatives thereof are one or more of high, medium and low-molecular weight chitosan, chitosan oligosaccharide, chitosan quaternary ammonium salt and carboxymethyl chitosan. The particle size of the proanthocyanidin sustained release nano-micro spheres is 10nm-0.3 mu m; the proanthocyanidin is prepared into the powder sustained release nano-micro spheres, and the proanthocyanidin sustained release nano-micro spheres can directly play a role in the lung as a pulmonary inhalation medicine-carrying formulation, improve the bioavailability of the proanthocyanidin and reduce the dosage by sustained release; the proanthocyanidin nano-micro spheres can also be applied as an oral sustained release formulation; the proanthocyanidin nano-micro spheres have appropriate particle size and morphology and other powder properties, as well as sustained release; furthermore, the preparation method has the advantages of simplicity and convenience in operation, stable process and easiness in industrial production.

The invention provides a preparation method of procyanidine. The preparation method includes the following steps: cleaning fresh humulus lupulus prior to airing for standby use; washing the aired humulus lupulus with organic solvent for three times, filtering with filtrate for standby application, and drying filter residue for standby application; crushing the dried filter residue to obtain coarse humulus lupulus powder; extracting the coarse humulus lupulus powder with acetone water solution for three times, and performing low-temperature pressure reduction to remove acetone so as to obtain humulus lupulus procyanidine coarse extract water solution; subjecting the humulus lupulus procyanidine coarse extract water solution to vacuum freeze drying, and washing with n-hexane and dichloromethane for two times respectively to obtain humulus lupulus procyanidine crude extract; subjecting the humulus lupulus procyanidine crude extract to mass spectrometry-mass spectrometry chromatography to obtain humulus lupulus polysaccharide, humulus lupulus anthocyanin low co-polymer and humulus lupulus anthocyanin high polymer. The preparation method of the procyanidine has the advantages that the low polymer and the high polymer of humulus lupulus anthocyanin can be obtained at the same time based on mass spectrometry-mass spectrometry chromatography, and the anthocyanin is high in purity and approximate in degree of polymerization.