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Analysis method for metabolin of A-type proanthocyanidins

A technology for metabolites and proanthocyanidins, which is applied to the analysis of materials, material separation, and preparation of test samples. It can solve the problems of complex components of A-type proanthocyanidins, low extraction recovery rate, and difficult detection and quantification. It achieves analytical methods Efficient and fast, reduce environmental pollution, high accuracy effect

Inactive Publication Date: 2012-07-04
HUAZHONG AGRI UNIV
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Problems solved by technology

However, if the metabolite components are complex and the content of the target substance is small, the extraction recovery rate will be low and the target substance will be interfered.
The components of A-type proanthocyanidins are complex. Compared with a single test substance, only using organic solvent extraction cannot completely separate and purify the metabolites, and it is not easy to detect and quantify

Method used

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  • Analysis method for metabolin of A-type proanthocyanidins
  • Analysis method for metabolin of A-type proanthocyanidins
  • Analysis method for metabolin of A-type proanthocyanidins

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Embodiment 1

[0024] A method for analyzing metabolites of A-type proanthocyanidins, comprising the following steps:

[0025] 1. Collection of biological samples

[0026] (1) Grouping: 15 male SD rats, weighing 200±20 g, were purchased from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology. The experiment was divided into 3 groups, normal group, low dose (150mg / kg) and high dose (300mg / kg) A-type proanthocyanidin group, 5 rats in each group.

[0027] (2) Administration: SD rats were raised in an SPF grade animal room for one week, and then the experiment was started. Dissolve 150 mg and 300 mg of freeze-dried A-type proanthocyanidin powder in 10 ml of normal saline to form a suspension, and give the rats in the low-dose and high-dose groups intragastric administration, and the normal group is directly replaced with normal saline. Rats were fasted for 12-18 hours before gavage, and gavage began at 4:00 p.m. After gavage, the rats were p...

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Abstract

The invention discloses an analysis method for metabolin of A-type proanthocyanidins, which comprises the steps of collection of a biological sample, preparation of metabolin solution, detection of the metabolin and the like, wherein the preparation of the mtabolin solution comprises preparation of hydrolysate, preparation of crude extraction of the metabolin and purification of the metabolin crude extraction. The analysis method for the metabolin of the A-type proanthocyanidins is good in separating purification effect, efficient, fast, high in result accuracy and good in reproducibility, and not only facilitates understanding of a metabolic mechanism and the metabolin, but also plays an important role in further utilization of the A-type proanthocyanidins.

Description

technical field [0001] The invention relates to an analysis method of A-type proanthocyanidin metabolites. Background technique [0002] Procyanidins (PC) is a general term for a large class of polyphenolic compounds that widely exist in the plant kingdom. Proanthocyanidins in nature are composed of different amounts of catechin and epicatechin. In different ways, it can be divided into two types: A-type (C2-O-C7 or C2-O-C5 connection) and B-type (C4-C8 or C4-C6 connection). A-type proanthocyanidins have strong antioxidant activities such as scavenging free radicals, inhibiting bacteria, lowering blood lipids, and inhibiting atherosclerosis, etc., and have great development and utilization value. A-type proanthocyanidins are absorbed into the blood through the small intestine of animals in vivo, and exist in the form of monomers and their methylation. After reaching the colon, they are degraded by intestinal microorganisms, forming a variety of phenolic acids and their deri...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N1/40G01N30/14
Inventor 谢笔钧孙智达李书艺肖娟隋勇刘亮周玮婧
Owner HUAZHONG AGRI UNIV
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