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Method for acquiring grape seed extract with absorption resin

A technology for adsorbing resin and grape seeds, used in organic chemistry and other directions

Inactive Publication Date: 2015-08-26
AMICOGEN CHINA BIOPHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the current extraction methods for high-content grape seed proanthocyanidin products can avoid the use of organic solvents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] This embodiment is a method for obtaining grape seed extract by using an adsorption resin, and its technological process is as follows: resin pretreatment, preparation of grape seed extract, purification of grape seed extract; purification of the grape seed extract Including purification of macroporous resin and purification of chromatographic resin.

[0015] The preparation process of the above-mentioned grape seed extract is as follows: load 60 mL of heavy 45 g macroporous adsorption resin, weigh 135 g of dried grape seeds of 3 times the mass, add 600 mL of deionized water, 0.27 g of 99.5 wt% glacial acetic acid, and stir for a while Extract in a water bath at 85°C for 90 minutes. After the extraction is completed, filter the extracted material and repeat the above steps to extract the grape seeds three times. Combine the extracts and centrifuge them at 4000r / min for 8 minutes. Filter paper to obtain the final extract, with an extraction rate of 4.3%, and adjust the p...

Embodiment 2

[0030] This embodiment is a feasible solution of Example 1, and its technological process is as follows: resin pretreatment, preparation of grape seed extract, purification of grape seed extract; the purification of described grape seed extract includes macroporous resin Purification and purification of chromatographic resins.

[0031] The preparation process of the above-mentioned grape seed extract is as follows: load 60 mL of heavy 45 g macroporous adsorption resin, weigh 135 g of dried grape seeds of 3 times the quality, add 600 mL of deionized water, stir slightly with 0.25 g of 99.5 wt % glacial acetic acid Extract in a water bath at 85°C for 90 minutes. After the extraction is completed, filter the extracted material and repeat the above steps to extract the grape seeds three times. Combine the extracts and centrifuge at 4000r / min for 8 minutes. The centrifuged liquid passes through a slow filter paper with a pore size of 1-3 μm The final extract was obtained with an ex...

Embodiment 3

[0046] This embodiment is a method for obtaining grape seed extract by using an adsorption resin, and its technological process is as follows: resin pretreatment, preparation of grape seed extract, purification of grape seed extract; purification of the grape seed extract Including purification of macroporous resin and purification of chromatographic resin.

[0047] The preparation process of the above-mentioned grape seed extract is as follows: load 60mL of heavy 45g macroporous adsorption resin, weigh 135g of dried grape seeds of 3 times the quality, add 600mL of deionized water, stir slightly with 0.28g of 99.5wt% glacial acetic acid Extract in a water bath at 85°C for 90 minutes. After the extraction is completed, filter the extracted material and repeat the above steps to extract the grape seeds three times. Combine the extracts and centrifuge them at 4000r / min for 6 minutes. The final extract was obtained, the extraction rate was 6.0%, and the pH was adjusted to 3.76.

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PUM

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Abstract

The method relates to the fields of plant extraction and healthcare foods, and especially relates to a method for extracting and purifying procyanidine from grape seed with macroporous absorption resin. The method comprises the specific processes of resin pretreatment, grape seed extract liquid preparation, and grape seed extract liquid purification. The grape seed extract liquid purification process comprises macroporous resin purification and chromatography resin purification. With the method, a finished product requirement of high purity can be achieved with the two-stage purification operation; in all processes, a basic concept of procyanidine high-purity product dependence on organic solvent is avoided to a maximal extent, and the extract liquid is completely purified with resin, such that residue caused by the application of organic solvent is avoided. The invention provides the green and environment-friendly procyanidine high-purity extraction method which is suitable for actual productions.

Description

technical field [0001] The invention belongs to the field of plant extraction and health food, in particular to a process for extracting and purifying proanthocyanidins from grape seeds by using a macroporous adsorption resin. Background technique [0002] Grape seeds contain a large amount of polyphenols. The grape seed extract we often refer to is generally proanthocyanidin polyphenols. Proanthocyanidins are polyphenolic compounds linked by monomer flavan-3-ols with different degrees of polymerization. , it is the substance with the strongest anti-oxidation and free radical scavenging ability found in nature, and it is a natural antioxidant. [0003] Grape seed proanthocyanidins extract contains many impurities, such as polysaccharides, macromolecular proteins, lipids, etc., which need to be further purified to obtain higher purity proanthocyanidins, and the commonly used purification methods include solvent extraction, membrane filtration and macroporous resin adsorption...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 刘庆文林炳旺李建国杨申勇王玲
Owner AMICOGEN CHINA BIOPHARM CO LTD
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