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A kind of preparation method of proanthocyanidin

A technology for thick proanthocyanidins and flower proanthocyanidins, which is applied in the field of preparation of proanthocyanidins, can solve the problem of low purity of extracted products, and achieve the effect of comprehensive utilization and reduction of environmental pollution

Active Publication Date: 2017-12-22
WEIHAI RENSHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of proanthocyanidins, to solve the problem of low purity of proanthocyanidin extraction products described in the background technology

Method used

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  • A kind of preparation method of proanthocyanidin

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Experimental program
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Effect test

Embodiment 1

[0029] The preparation method of proanthocyanidins provided by the embodiments of the present invention comprises the following steps:

[0030] S101: washing the fresh hops and drying them for later use;

[0031] S102: washing the dried hops three times with an organic solvent, filtering, and using the filtrate for later use, and drying the filter residue for later use;

[0032] S103: pulverize the dried filter residue to obtain coarse hop powder;

[0033] S104: Extract the coarse hop powder with an aqueous acetone solution for 3 times, remove the acetone under reduced pressure at low temperature, and obtain a crude aqueous solution of hop proanthocyanidins;

[0034] S105: Vacuum freeze-dry the aqueous solution of crude hop proanthocyanidins, and wash twice with n-hexane and dichloromethane, respectively, to obtain crude hop proanthocyanidin extracts;

[0035] S106: The hop proanthocyanidin crude extract is chromatographed by tandem mass spectrometry to obtain hop polysaccha...

Embodiment 2

[0037] S201: washing the fresh hops and drying them for later use;

[0038] S202: Wash the dried hops 3 times with an organic solvent whose mass is 50 times the mass of fresh hops, filter, set aside the filtrate, and dry the filter residue for use;

[0039] S203: pulverize the dried filter residue to obtain coarse hop powder;

[0040] S204: Extract the coarse hop powder with an aqueous acetone solution for 3 times, remove the acetone under reduced pressure at low temperature, and obtain a crude aqueous solution of hop proanthocyanidins;

[0041] S205: Vacuum freeze-dry the aqueous solution of crude hop proanthocyanidins, and wash with n-hexane and dichloromethane twice respectively, to obtain crude hop proanthocyanidin extracts;

[0042] S206: The hop proanthocyanidin crude extract is chromatographed by tandem mass spectrometry to obtain hop polysaccharides, hop anthocyanin low copolymers and hop anthocyanin high polymers.

Embodiment 3

[0044] S301: washing the fresh hops and drying them for later use;

[0045] S302: Wash the dried hops 3 times with dichloromethane whose mass is 50 times that of fresh hops, filter, distill the filtrate under reduced pressure, add petroleum ether, filter, wash, and concentrate to obtain a petroleum ether concentrate containing hop chlorophyll, The petroleum ether concentrate containing hop chlorophyll is used for subsequent use, and the filter residue is dried for subsequent use;

[0046] S303: pulverize the dried filter residue to obtain coarse hop powder;

[0047] S304: extracting the coarse hop powder with an aqueous acetone solution for 3 times, removing the acetone under reduced pressure at a low temperature, and obtaining an aqueous solution of crude hop proanthocyanidins;

[0048] S305: Vacuum freeze-dry the aqueous solution of crude hop proanthocyanidins, and wash with n-hexane and dichloromethane twice respectively, to obtain crude hop proanthocyanidin extracts;

[...

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Abstract

The invention provides a preparation method of procyanidine. The preparation method includes the following steps: cleaning fresh humulus lupulus prior to airing for standby use; washing the aired humulus lupulus with organic solvent for three times, filtering with filtrate for standby application, and drying filter residue for standby application; crushing the dried filter residue to obtain coarse humulus lupulus powder; extracting the coarse humulus lupulus powder with acetone water solution for three times, and performing low-temperature pressure reduction to remove acetone so as to obtain humulus lupulus procyanidine coarse extract water solution; subjecting the humulus lupulus procyanidine coarse extract water solution to vacuum freeze drying, and washing with n-hexane and dichloromethane for two times respectively to obtain humulus lupulus procyanidine crude extract; subjecting the humulus lupulus procyanidine crude extract to mass spectrometry-mass spectrometry chromatography to obtain humulus lupulus polysaccharide, humulus lupulus anthocyanin low co-polymer and humulus lupulus anthocyanin high polymer. The preparation method of the procyanidine has the advantages that the low polymer and the high polymer of humulus lupulus anthocyanin can be obtained at the same time based on mass spectrometry-mass spectrometry chromatography, and the anthocyanin is high in purity and approximate in degree of polymerization.

Description

technical field [0001] The invention relates to the technical field of biological extraction, and more specifically, relates to a preparation method of proanthocyanidins. Background technique [0002] Proanthocyanidin, also known as vitamin P, is a bioflavonoid with a special molecular structure. The main components of proanthocyanidins are catechins, which account for 65-80% of the total proanthocyanidins. According to the degree of polymerization, di-pentamers are usually called oligomeric proanthocyanidins, and those with pentamers and above are called high proanthocyanidins. polyproanthocyanidins. Proanthocyanidins have various physiological functions such as antibacterial, anti-oxidation, and anti-free radicals. They are the "health guards" of nature and human beings. They are considered as the seventh "nutrient" indispensable to human beings and can be used as raw materials for health care products. Proanthocyanidins have strong anti-oxidation and anti-free radical a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C08B37/00
CPCC07D311/62C08B37/0003
Inventor 苗其壮关键
Owner WEIHAI RENSHENG PHARMA GRP
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