The invention discloses a preparation method of up-conversion
luminescence coding nano composite particles based on
mesoporous silica, which comprises the following steps: preparing
metal fluoride precursor
sol containing
rare earth elements, stirring to enable the
sol to enter pore channels of the
mesoporous silica through a capillary effect, centrifuging at a
low speed to remove a supernate, adding water, and carrying out soft vortex oscillation , and washing off the precursor
sol on the surfaces of the particles, centrifugally collecting a sample at a
low speed,
drying the sample in a
drying oven to form xerogel of
metal fluoride in the pore channels of the
mesoporous silica, and
sintering the xerogel after
drying to obtain the up-conversion luminescent mesoporous silica
composite nanoparticles. According to the method, the up-conversion luminescent nanocrystals can only nucleate and grow in
silicon oxide mesopores, the biotoxicity of the up-conversion luminescent
nanocrystal encoding microspheres can be greatly reduced, and further modification of the encoding microspheres is facilitated; the invention also provides a corresponding coding method, so that the types or contents of
rare earth elements contained in the up-conversion luminescent nanocrystals in different composite particle pore channels are different.