47results about How to "Specific response" patented technology

The invention relates to the field of chemical synthesis, particularly to a novel synthetic process of an antibiotic material drug special for animals, in particular to a novel synthetic method of cefquinome sulfate. The method comprises the following steps of: taking GCLE (7-Phenylacetamide-3-chlorormethyl-3-cepham-4-carboxylic acid p-meth-oxybenzyl ester) as a starting material, and reacting with 5,6,7,8-tetrahydroquinoline after chlorine is replaced by iodine in C-3 chlorine methyl; by preparing an intermediate (7), successively stripping C-7 amino protection and C-4 carboxyl protection under the action of phosphorus pentachloride and phenol; reacting with AE-active ester in the existing of triethylamine; and preparing cefquinome sulfate. The method effectively solves the problems of stringent requirements of reaction condition of an original process route, complicated reaction system, difficult separation and purification, low purity and bad tincture of products and the like. The method has the advantages of simple technology, mild reaction condition, high yield, low cost and high quality and is suitable for industrialized production; and raw auxiliary materials have low priceand are easily obtained.

The invention discloses a method for preparing cholesterol by a microbial conversion method. The method is characterized in that cholesterol is prepared from raw material lanolin completely by microbial conversion through the steps of: 1) crushing raw material and adding a carbon source and a nitrogen source to prepare a seed medium and a fermentation medium; 2) screening strains; 3) inoculating the screened strain and then stirring and fermenting; and 4) separating, purifying and crystallizing. The method has the characteristics of mild conditions, specific reaction, small pollution and the like in preparing cholesterol. The cholesterol prepared by the method has high purity and yield, and conforms to the concept of environment protection.

The invention provides a preparation method of 5,6-dihydropyridine-2(1H)-one derivatives, specifically 1-(piperidin-2-one-1-yl)-4-(5,6-di The preparation method of hydrogen-3-R substituent pyridine-2(1H)-one-1-yl)benzene, the R substituent is chlorine or morpholin-4-yl. The present invention uses p-acetamidoaniline as a raw material, undergoes amidation with δ-valerolactone, halogenates with a halogenating agent or sulfonylates with sulfonyl chloride, condenses, deacetylates, and then reacts with 2,2-dichloro-δ - Amidation of valerolactone, halogenation with halogenating reagents or sulfonylation with sulfonyl chloride, followed by condensation elimination or condensation elimination substitution in the presence of morpholine to prepare the target product. The raw materials used in the preparation method of the invention are cheap and easy to obtain, and the cost is low; the process is simple and easy to operate, the reaction conditions are easy to realize, the amount of waste water generated is small, and it is safe and green; the reaction selectivity of each step is high, the product yield and purity are high, and it is beneficial to industrial production.

The invention belongs to the field of biological detection, in particular relates to a method for detecting enzymatic activity by using quantum dot fluorescence. The method provided by the invention is used for detecting the enzymatic activity through measuring the influence of a reaction system to water-soluble quantum dot fluorescence property in the enzyme reaction process. The method concretely comprises the following steps of: adding a certain quantity of enzymes to be detected in a reaction system containing water-soluble quantum dots or further in a reaction system containing coenzymes, enabling the fluorescence intensity of the water-soluble quantum dots to be varied due to the reaction of the enzymes to be detected, and realizing the high selective quantitative detection on the activity of the enzymes to be detected through the variable quantity of the fluorescence intensity of the water-soluble quantum dots. The method provided by the invention is simple in operation, rapid to detect and low in cost and can be used for detecting the activity of various physiological enzymes relevant to disease diagnosis.

The present invention discloses a total bilirubin determining reagent box, which is liquid double reagent formed by reagent 1 and reagent 2. The constituents and the consistence range of the reagent 1 are: disodium of 13.5-81g / L, sodium dihydrogenphosphate of 0.6-3.6g / L, sodium chloride of 9.0g / L, ethylenediaminetetraacetic acid disodium salt of 0.2-1g / L, tween of -80 0.5-2ml / L, and sodium dodecyl sulfate of 1-10g / L. The constituents and the consistence range of the reagent 2 are: disodium of 1g / L, sodium chloride of 9g / L, sodium persulfate of 11.9-23.8g / L, and sulphuric acid of 0.1-2ml / L. The total bilirubin determining reagent box is stable and environment-friendly, and has proper linearity range, high test accuracy, high precision, high sensitivity and strong anti-jamming performance.