153 results about "7-ACA" patented technology

The invention discloses a CPC acylation enzyme whose encode gene is DNA sequence shown as SEQ IN NO: 4 and DNA sequence having at least 80154000mology with the above DNA sequence. The invention also discloses carriers and transformants containing coding CPC acylation enzyme and the use of the enzyme. The enzyme has high expressing activity, high catalytic activity and high outcome tolerance to catalysis bottom object in high efficiency to prepare outcome 7-ACA.

The invention relates to a synthetic method of antibiotic cefoxitin. 7-ACA is taken as a starting material and firstly reacts with thiopheneacetyl chloride, cephalothin acid is obtained by separation; the cephalothin acid is not purified, and the methoxylation is directly carried out on the cephalothin acid to obtain an intermediate A which introduces methoxy on the position of 7, the intermediate A obtains 7-alpha-methoxy cephalothin cyclohexylamine salt under the action of cyclohexylamine; the 7-alpha-methoxy cephalothin cyclohexylamine salt reacts with benzathine diacetate under the catalysation of solid alkaloid to obtain 7-alpha-methoxy-3-deacetoxy cephalothin benzathine salt; and the carbamylation is carried out under the action of chlorosulfonyl isocyanate to obtain the cefoxitin. The synthetic method has the advantages that compared with the prior art, the synthetic method simplifies the operation process, has high product yield, reduces the production cycle, eliminates the discharge of waste water containing organic solvent and reduces the production cost. The product quality is stable, and the synthetic method is applicable to the large-scale industrial production.

The preparation process of cefathiamidine includes the following two synthesis steps: 1) preparing cefathiamidine acid through the reaction between 7-ACA and bromoacetyl bromide, acidifying, solvent extraction, concentration and drying; and 2) preparing cefathiamidine product through adding alkali reagent to dissolve cefathiamidine acid, reaction with diisopropyl thiourea, and adding semi-soluble solvent to separate out cefathiamidine product. The said preparation process has less steps, and is simple and feasible.

The invention provides a process for recovering a 7-ACA (aminocephalosporanic acid) product from mother liquor through macroporous adsorption resin. The process mainly comprises the following steps: performing resin adsorption, performing resin desorption, concentrating and the like, wherein in the resin absorption step, the pH value of 7-ACA crystallization mother liquor is adjusted, the crystallization mother liquor passes through a macroporous adsorption resin layer from top to bottom at a certain flow speed, and when the valence of 7-ACA contained in effluent of a resin column is 1% of that of the crystallization mother liquor at an inlet, resin adsorption is stopped. According to the method, the qualified 7-ACA product with the purity of greater than or equal to 98% and the color grade of greater than 4 can be obtained; the extraction yield of the resin is greater than or equal to 90% and the total process yield is greater than or equal to 40%; based on estimation of 200t of the mother liquor produced each day, the economic benefit of a year can reach more than ten million yuan.

The invention discloses a method for preparing cefuroxime acid. The method comprises the following steps of: performing a chloride acylation reaction on 7-aminocephalosporanic acid (7-ACA) and methoxyiminofuran acetate serving as raw materials; performing deacetylation to synthesize DCCF; performing nucleophilic addition on the DCCF and chlorosulfonyl isocyanate (CSI) serving as a strong carbamoyl reagent to obtain chlorosulfonyl cefuroxime; and hydrolyzing the chlorosulfonyl cefuroxime to obtain cefuroxime acid. In the preparation method, the preparation process is simple, and the cefuroximeacid is crystallized by adopting aqueous solution, so that the loss of organic solvents is reduced; simultaneously, aids are added selectively in the reaction process to improve the quality of products, so that finished products with high purity and yield and good colors are obtained. The purity of the cefuroxime acid prepared by the method is more than 98.5 percent, and the weight yield is approximately 100 percent.

The invention relates to a synthesis method of an intermediate of methoxyl cephalosporin. C3 site methyl halide of a cephems compound with C4,7 site being protected, instead of the ordinary 7-ACA, is used as a material, functionalized at C3 site, and then methoxylated at C7 site, finally de-protected, and the intermediate of methoxyl cephalosporin is prepared. The compound can be conveniently used in the synthesis of various methoxyl cephalosporin medicines. The invention has apparent advantages of clear synthesis route, less steps, less and inexpensive reagents, comparatively single solvent, convenience for recovery and recycling, low material cost and operation expenses.

The invention is a method of preparing D-amino acid oxidase, relating to a method of preparing flavo-enzyme D-amino acid oxidase. Concretely, it relates to a method of highly efficiently producing mutational D-amino acid oxidase by constructing recombinant engineering strains. It reconstructs wild D-amino acid oxidase coming from Trigonopsis variablilis by means of gene engineering, fuses a segment of Histag at N end and C end of the wild enzyme, and further implements high-efficiency fast separation of mutational enzyme by affinity chromatography. The expression level of the enzyme in fermentation liquor is 4000IU/mL, higher than that of the wild enzyme, and the recovery ratio of Ni-column affinity chromatography is 50%. The purified enzyme can be used in making high-efficient oxidation conversion of cephalosporin C- to produce glutaryl-7-ACA and be used together with GL-7-ACA to produce 7-ACA.

The invention relates to a preparation technology of cefotaxime, belonging to the preparation technology of medical compounds. The technology comprises the following steps: adding 7-ACA and AE-active resin reagents in solvent, and then adding an organic base and an alcohol catalyst to carry out a synthesis reaction. The preparation technology is characterized in that an acidifier is directly added for acidification after the reaction is finished so as to devitrified. The invention has simple and convenient operation, short production period, high product yield and low solvent consumption and can obtain products with uniform crystallized grains, good colors, high purity, low cost and stable quality.

The invention discloses synthesis of antibiotic ceftazidime, ceftazidime for injection and a preparation method of ceftazidime. 7-ACA is served as a raw material, silanization and iodo reaction are carried out, and pyridine, hydrochloric acid and water are added to obtain TA (7-PyCA); ceftazidime active ester is added in an organic solvent and an inorganic solvent to obtain new modified ceftazidime active ester; TA is added in new modified ceftazidime active ester, organic mixed solvent and triethylamine are added, then acid water and an acetone solution are added to obtain TC (ceftazidime dihydrochloride), and ceftazidime is obtained through the reaction. The synthesis of ceftazidime provided by the invention has the advantages that ceftazidime is high in yield, operation processes are simplified, production cost is low, ceftazidime is applicable to industrial production, obtained products can be stored for a long time, a structure is stable, and impurity content is low. Ceftazidime for injection has the advantages of being moderate in grain size, good in clarity, less in impurity content, excellent in quality, stable and the like, and is high in split charging efficiency in production, good in content uniformity, high in medicine dissolution rate in clinical application and good in dissolubility.

The invention relates to the field of the synthesis of chemical medicaments and discloses a preparation method of cefathiamidine; the method takes chloracetyl chloride as a raw material and comprises the following steps: (1) on the condition of the presence of a solvent, alkali is added so as to cause 7-ACA to be dissolved, and then the chloracetyl chloride is added for a condensation reaction; after the condensation reaction is finished, chloracetyl 7-ACA crystals are separated out with an acid; and (2) on the condition of the presence of both the solvent and a catalyst of a catalyzing amount, the chloracetyl 7-ACA reacts with N, N-di-isopropyl thiourea to produce the cefathiamidine. Besides the advantages of bromoacetyl-bromide preparation method of cefathiamidine, the technology of adopting chloracetyl chloride as the raw material to produce the cefathiamidine also has the advantages that: as no alkali is added into the reaction between the chloracetyl 7-ACA and the N, N-di-isopropyl thiourea, the produced cefathiamidine has lighter color, and better and more stable quality, is more beneficial to store and transport, improves the overall yield, lowers the cost and has broader prospects; and the price of the chloracetyl chloride is one sixth of that of the bromoacetyl bromide, which significantly reduce the cost.

The invention relates to a synthesis art of a chemical product, in particular to a new synthesis art for 3-deacetyl cefuroxime acid (DCCF). The art of the invention uses SMIA acyl chloride and 3-deacetyl cephalosporin acid (D-7-ACA) as the main synthesis ingredients to replace the SMIA acyl chloride and the 7-ACA, which reduces the chemical reaction process needed in the synthesis, shortens the production cycle, reduces the using solvent species, facilitates the solvent recovery and improves the product synthesis rate.

The invention discloses a preparation method of polyvinyl alcohol immobilized acylase. The preparation method comprises the steps: preparing a mixed aqueous solution from polyvinyl alcohol and a cross-linking agent, adding acylase into the mixed aqueous solution of the polyvinyl alcohol and the cross-linking agent, uniformly mixing to obtain an acylase polyvinyl alcohol gel aqueous solution, and carrying out formation and solidification process treatment on the acylase polyvinyl alcohol gel aqueous solution to obtain the polyvinyl alcohol immobilized acylase. The polyvinyl alcohol immobilized acylase is used for synthesizing a cephalosporin antibiotics midbody 7-ACA (Aminocephalosporanic Acid) or 7-ADCA (Aminodesacetoxycephalosporanic Acid). According to the preparation method disclosed by the invention, as PVA (Polyvinyl Alcohol) is used for immobilizing biological enzyme, the enzyme activity recovery rate can reach above 80 percent, immobilized acylase is also more stable and can be reused for above 50 times, so that the application cost of the acylase in the production of the 7-ACA and the 7-ADCA is greatly lowered, and the production efficiency and the product quality are improved.

The invention relates to a method for preparing 3-descarbamoyl-cefuroxime acid. The method comprises the following steps of: (1) dissolving 7-aminocephalosporanic acid (7-ACA) in water or a methanol solution to prepare a 7-ACA solution, and performing hydrolysis reaction to obtain a 3-deacetyl-7-aminocephalosporanic acid (7-DACA) solution; (2) dissolving an acylchlorination reagent in a solvent, adding a cosolvent, adding (Z)-2-methoxyimino-2-(furyl-2-yl)acetic acid ammonium salt (SIMA), reacting, filtering, adding water or performing rotary evaporation to remove the excessive acylchlorination reagent, performing vacuum rotary evaporation, adding a homogenization reagent, and dissolving to obtain a (Z)-2-(furyl-2-yl)-2-(methoxyimino)-acetyl chloride (SMIF-Cl) solution; (3) adding the homogenization reagent into the 7-DACA solution, dripping the SMIF-Cl solution, regulating the pH value, preserving heat and reacting to obtain a reaction solution; and (4) decolorizing the reaction solution, regulating the pH value, adding purified water, growing a crystal, filtering, and performing vacuum drying. Reactants form a homogeneous system by adding the homogenization reagent, so that the contact area of the reactants is expanded, the reaction rate is increased, and reaction time is shortened.

The invention relates to a method for preparing 7-ACA (aminocephalosporanic acid) and obtaining alpha-aminoadipic acid. The method comprises the following steps of (1) treating cephalosporin C fermentation liquid; (2) performing an enzymolysis reaction of cephalosporin C under the effect of cephalosporin C acyltransferase to obtain 7-ACA; (3) treating the 7-ACA lysate after the enzymolysis; (4) separating out the 7-ACA; (5) separating alpha-aminoadipic acid from the separated 7-ACA lysate.

The invention relates to a synthesis method of cefepime dihydrochloride that is a bacteriophage. 7-amin cethalosporanic acid (7-ACA) is used as starting material and reacts with hexamethyldisilane amine (HMDS) and iodotrimethylsilane (TMSI) first to obtain 7-ACA for protecting amino and carboxyl; then 7-ACA, amino and carboxyl of which are protected, reacts with iodotrimethylsilane and N-methylpyrrolidine to synthesize (6R, 7R)-7-amino-3-((1-methyl-1-pyrrolidine) methyl) cephalosporin-3-alkene-4-carboxylic acid hydrochloride (7-MPCA) through a one-pot method; 7-MPCA reacts with AE active ester to obtain a product of cefepime dihydrochloride through acidylation reaction and salifying reaction. Compared with the existing technical route, the synthesis method has the advantages that the process conditions are simple, the operation is convenient, the product yield is high, the product quality is stable, the method is suitable for the large-scale industrialized production, etc.

The method for preparing glutaryl-7-aminocephaphylanic acid acylase is characterized by that it utilizes colibacillus gene engineering bacterium to make shake-flask strain culture in culture medium, fermentation strain culture and fermentation culture, then utilizes centrifuge to collect thallus, soakes to extract enzyme liquor, makes the enzyme liquor undergo the processes of plate-frame filtration, ultrafiltration concentration, purification and fixation so as to obtain the invented product GL-7-ACA ACY.

The invention relates to a preparation method for a cephalosporin anti-infective drug-cefazedone sodium, belonging to the field of pharmaceutical synthesis. According to the invention, the method uses GCLE as a raw material to substitute 7-ACA and overcomes the defects of low yield, high pollution and the like in prior art; the preparation method with mild reaction conditions, little side reaction and simple process is provided; meanwhile, the method has the advantages of cheap and easily-available raw materials, low cost, high product yield, high product purity and applicability to industrial production.

Disclosed is a process for preparing cefotaxime sodium belonging to compound preparation technical field. In a solvent, 7-ACA reacts with AE-active ester under the action of amine intermediate reactant, then a sodium salt forming agent is added in, and the cefotaxime sodium is obtained after reaction and seedout. The invention is characterized in that the solvent is a mixed solvent composed of benzene organic solvent, ethyl acetate, or acetone and alcohol organic solvent. The technological process is simplified, and the product yield is high.

The invention belongs to the technical field of pharmacy, and relates to a comprehensive recovery method for effective ingredients in 7-amidogen cephalosporins alkanes acid crystallization mother liquor produced through an enzymatic hydrolysis method. The method comprises the following steps that 1, the 7-ACA mother liquor is prepared; 2, 7-ACA and CPC are adsorbed and decomposed; 3, D-alpha-aminoadipicacid is prepared; 4, 7-ACA is prepared. 7-ACA and D-alpha-aminoadipicacid in the 7-ACA crystallization mother liquor are separated through the specific adsorption effect of macroreticular resin on 7-ACA, appropriate decomposition agents is adopted for decomposing 7-ACA and CPC adsorbed to the resin, then D-alpha-aminoadipicacid and 7-ACA are recovered from adsorption raffinate and decomposition liquid respectively, the quality of the recovered 7-ACA products is superior to that of 7-ACA products obtained through the enzymatic hydrolysis method, and D-alpha-aminoadipicacid can be used for preparing a fermentation medium of CPC. The effective ingredients in the mother liquor are successfully recovered through the brand-new process, the emission of high-concentration waste water is greatly reduced, the cost for treating sewage is reduced, and environment-friendly, clean and green production is achieved.