The invention discloses a method for preparing a quantum dot / enzyme composite carbon paste electrode for detecting SAM, which is characterized in that: carbon paste electrodes are doped with water-soluble CdZnTe quantum dots and methyltransferase, and 1-ethyl ‑3‑Methylimidazole hexafluorophosphate is used as an adhesive to prepare a carbon paste electrode by mixing carbon nanotubes and graphite powder, which is a quantum dot / enzyme composite carbon paste electrode. Compared with ordinary carbon paste electrodes, the conductivity is improved by 1~2 times, and the electrochemical window is wide, the preparation method is simple, the cost is low, the surface is easy to renew, and the residual current is small; the electrode is used to quickly detect SAM in the sample, and the method has high sensitivity. Good selectivity, short response time, less interference, superior to other detection methods, is a simple, fast, convenient and easy SAM determination method, the fixed methyltransferase electrode sensor prepared by the application has low cost, simple preparation process, specific It has good performance and has the possibility of realizing automatic on-site determination.