The invention discloses an acidic silicone sealant with good bonding performance on stainless steel, and a preparation method thereof. The sealant comprises the components of, by weight: 20-90 parts of a,w-dihydroxy polysiloxane, 2-10 parts of a plasticizer, 10-50 parts of a filling material, 3-10 parts of a thixotropic agent, 2-10 parts of a cross-linking agent, 1-5 parts of a tackifying agent, and 0.1-5 parts of a catalytic curing agent. Compared with prior arts, according to the invention, the novel tackifying agent is added, such that the sealant has good bonding performance on stainless steel. A silver-grey colorant is added into an acidic transparent system, such that the sealant has a shiny color that is substantially similar to that of stainless steel. Because the sealant is an acidic silicone sealant, curing speed is high. With the sealant, production efficiency can be substantially improved, bonding performance can be improved, and glass and stainless steel falling can be prevented. During the production process, energy consumption and water consumption are low, no three-waste (waste gas, waste water, and solid waste) is produced, and no environment pollution is caused. The sealant and the method has significant economic and social benefits.