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Preparation method of cellulosic ethanol

A technology of cellulosic ethanol and lignocellulose, which is applied in the field of cellulosic ethanol preparation, can solve the problems of cumbersome preparation process, waste of resources, high energy consumption and water consumption, and achieve simplified preparation process, saving water and energy consumption, reducing The effect of preparation costs

Pending Publication Date: 2021-04-06
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, compared with petroleum and grain cellulosic ethanol, the current market competitive advantage of cellulosic ethanol is not strong, and the cost still restricts its industrial scale development
Since the conversion of lignocellulose into cellulosic ethanol requires pretreatment, enzymolysis, fermentation and other processes, most pretreatment methods have poor biocompatibility, and certain pretreatment of biomass is required before enzymolysis and fermentation, such as the patent CN101787384A discloses a method for coupling enzymatic hydrolysis and saccharification of lignocellulosic raw materials, but this method requires multiple filtrations, washings, dilutions, etc., so the preparation process is not only cumbersome, time-consuming, energy consumption and water consumption are relatively high, there is waste of resources and The problem of difficult disposal of waste and the lack of cost-effective pretreatment technology will inevitably make the cost of cellulosic ethanol production process remain high

Method used

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  • Preparation method of cellulosic ethanol
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Examples

Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1 A kind of preparation method of cellulosic ethanol

[0048] S1. Grinding and sieving the bagasse, taking 3g of the crushed bagasse raw material (0.3mm in crushed particle size) and mixing it with 60mL of water (1:20) and putting it in a reactor for reaction at a reaction temperature of 210°C. The reaction time is 0 min, and after the reaction is completed, the mixture is cooled in cold water to obtain bagasse mixture liquid A.

[0049]Wherein, the reaction time is counted from when the temperature is raised to the reaction temperature, and when the reaction time is 0 min, it means that the reaction is stopped immediately when the temperature is raised to the reaction temperature.

[0050] S2. Add the feed liquid A obtained in S1 to a NaOH solution with a concentration of 4mol / L to adjust the pH to 5, and add 36FPU / g 甘蔗渣原料 Cellulase and 7000FXU / g 甘蔗渣原料 Put xylanase in a constant temperature shaker at 50°C for 72 hours of enzymolysis, obtain enzymolysis sol...

Embodiment 2

[0052] Embodiment 2 A kind of preparation method of cellulosic ethanol

[0053] The solution is the same as in Example 1, except that the biomass raw material in step S1 is corn cobs, and the crushed particle size is 0.9 mm.

Embodiment 3

[0054] Embodiment 3 A kind of preparation method of cellulosic ethanol

[0055] Same as the solution in Example 1, except that the biomass raw material in step S1 is rice straw, and the pH in step S2 is 4.5.

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Abstract

The invention discloses a preparation method of cellulosic ethanol, which comprises the following steps of pulverizing lignocellulosic biomass, carrying out hydrothermal reaction, cooling to obtain a mixed solution, regulating the pH value, adding cellulase and xylanase, carrying out enzymolysis, inoculating yeast, and fermenting to prepare cellulosic ethanol. According to the method, hydrothermal short-time depolymerization pretreatment is utilized to obtain the low-toxicity pretreatment liquid, and the cellulosic ethanol is prepared by a one-pot method, so that the operation steps are simplified, xylan in the pretreatment liquid is retained, pentose and hexose co-fermentation is realized, the yield of the cellulosic ethanol is remarkably increased, and the method is low in cost, low in energy consumption, free of wastewater discharge and effective sugar components in lignocellulose can be comprehensively utilized.

Description

technical field [0001] The invention relates to the technical field of cellulosic ethanol preparation, in particular to a method for preparing cellulosic ethanol. Background technique [0002] The rapid development of the world economy is inseparable from the use of fossil energy such as coal, oil, natural gas and nuclear fission energy, but it is accompanied by increasingly severe environmental problems, such as the depletion of fossil resources, the global temperature rises year by year, and the ecological environment. getting worse. Recently, the "2020 Energy Technology Outlook Report" issued by the International Energy Agency pointed out that at present, the harmful greenhouse gases emitted by the energy industry account for about 1 / 3 of the world. The platoon task is arduous. The development of renewable energy to continuously promote energy transformation and structural adjustment is the priority choice to deal with the current energy crisis. [0003] As a new type ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12P19/02C12R1/865
CPCC12P7/10C12P19/14C12P19/02C12P2201/00Y02E50/10
Inventor 林晓清郑晓洁刘耀陶顺辉胡蕾张晓东冼君亭
Owner GUANGDONG UNIV OF TECH
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