The invention provides a preparation method of a microcrystalline glass applied to a 5G communication mobile terminal, and belongs to the technical field of microcrystalline glass. The preparation method comprises following steps: step A, preparing following raw materials in parts by weight: 45 to 75 parts of quartz sand, 10 to 25 parts of aluminum oxide, 14.5 to 39.6 parts of sodium carbonate, 2.3 to 9.2 parts of potassium nitrate, 0 to 12.5 parts of lithium carbonate, 0 to 41 parts of magnesium carbonate, 0 to 8 parts of titanium oxide, 0 to 20 parts of zirconium oxide, 0 to 10 parts of zincoxide, 0 to 3 parts of rubidium oxide, 0 to 5 parts of gallium oxide, 0 to 3 parts of europium oxide, 0 to 9 parts of ammonium dihydrogen phosphate, 0 to 3 parts of antimony oxide, 0 to 3 parts of yttrium oxide, 0 to 3 parts of cerium oxide, 0 to 5 parts of iron oxide, 0 to 2 parts of manganese oxide, 0 to 3 parts of nickel oxide, and mixing all raw materials to obtain a mixture; step B, meltingthe mixture; step C, moulding the melt; and step D, carrying out annealing, nucleation, and crystallization to obtain the microcrystalline glass. By controlling the raw materials and technology, the obtained front cover microcrystalline glass has high transmittance of visible light, high strength, and high hardness, and the obtained rear cover microcrystalline glass has high strength and low magnetic loss.