51 results about "Rhododendron weyrichii" patented technology

The invention provides a separation and purification method of a rhodojaponin-III monomer, and belongs to the technical field of separation and purification of active ingredients of traditional Chinese medicines. The separation and purification method comprises the following steps in sequence: based on ericaceous plant rhododendron molle as a raw material, carrying out alcohol extraction, ethyl acetate extraction and preparative high performance liquid chromatography separation; and concentrating and drying to obtain the rhodojaponin-III monomer. According to the preparation method, the optimal technology and parameter conditions are utilized, thus the content of the rhodojaponin-III in the product is up to more than 98%; and the method is simple and convenient for operation in the whole technological process, stable in technology, low in cost, suitable for mass production, high in separation efficiency and high in purity of product, and can realize high-purity separation and preparation of the rhodojaponin-III monomers as mass.

The invention relates to plant propagation and in particular relates to a method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in the Changbai Mountains. Taking rhododendron aureum as example, the method comprises the steps of: (1) collecting branches of the rhododendron aureum in late October, carrying out low-temperature dormancy on the branches, carrying out water planting to promote the germination and the growth of lateral buds and cutting in a form of one segment with one leaf after the treatment to be used as explants for later use, wherein the culture medium for the rooting of the stem segments and the germination and the growth of the lateral buds comprises the components of: the basic culture medium+ 0.13-0.14mg.L-1 of KT (kinetin)+ 0.05-0.08mg.L-1 of IBA (Indole Butyric Acid)+ 0.02-0.05mg.L-1 of NAA (Naphthyl Acetic Acid)+ 2.70-2.90mg.L-1 of GA(Gibberellin)3. According to the method, the process is simple, the cost is low, the operability is strong and the capability of improving the seedling producing efficiency is high, so that the method can be directly applied to industrial seedling cultivation of the rhododendra.

The invention relates to a bacteria liquid for growth and development of plants, and in particular relates to a preparation method for bacteria liquid beneficial for growth-development and mycorrhiza formation of rhododendron plants. The method comprises the following steps: (1) preparation and treatment of materials: collecting and disinfecting mature fibrous roots of rhododendron plants, and cutting the treated mature fibrous roots to root segments for later use; (2) culture medium configuration: the content of 1000mL of culture medium comprises the following components of 380mg of KH2PO4, 149mg of MgSO4.7H2O, 26mg of vitamin C, 67mg of calcium pantothenate, 1.4mg of nicotinic acid, 3.0g of peptone, 16.5g of glucose, 8.5g of agar powder, 4mL of 1.5% of sodium deoxycholate liquid and 0.05mg of indoleacetic acid; (3) preparation of mother liquid; and (4) preparation of the bacteria liquid. The preparation method provided by the invention provides a technical method for production, application and fundamental research on introduction, artificial breeding, cultivation, breeding and the like of the rhododendron plants in Changbai Mountain and other rhododendron plants at home and abroad.

A method for identifying a rhododendron hybrid variety based on an EST-SSR molecular marker comprises the following steps of amplifying samples of the rhododendron hybrid variety by adopting a plurality of pairs of primers such as R26, R28, R29, S1, S14 and the like, then comparing amplified bands of all samples to be tested by utilizing a fluorescent capillary electrophoresis method, and identifying the rhododendron hybrid variety through comparison. The average allele number (Na), the Shannon's information index (I) and the polymorphic information content (PIC) of the 15 pairs of screened primers are 6.3333, 1.2977 and 0.5767 respectively, rich and stable polymorphism is shown, particularly, the five pairs of primers S14, S1, R29, R28 and R26 have the highest efficiency, can be used as core primers for screening hybrid offspring. And genetic diversity analysis and molecular marker-assisted breeding identification of different hybridized combinations and even distant hybridized combinations are facilitated.

The invention discloses a traditional Chinese medicine preparation for lowering blood pressure, which is prepared from oriental paperbush flower, self-heal, fruit of negundo, corn silk, Fructus Forsythiae, radix sileris, herba houttuyniae, burdock fruit, Plantain seed, cornel, rhizoma atractylodis, radix clematidis, lobelia chinensis, rhododendra, fructus psoraleae, sophora flower, saffron, Cortex Fraxini, ephedra, ginger, cherokee rose, Semen Allii Tuberosi, Chinese anise, small fennel, fructus piperis longi, Rhododendron molle, Strophanthus divaricatus, eupatorium and nutmeg. The preparation can achieve the goals of favorable curative effect and no side effect when being used for treating hypertension, and all the active pharmaceutical ingredients are cheap and accessible.

The invention relates to a method for cultivating an early blossoming and multiplicate rhododendron. The method comprises the following steps of: selecting rhododendron plants which do not have plant diseases and insect pests, are robust in growth vigor and are in the early flowering period, and are used as a cross breeding parent, namely Hanyin No.2 (female)* holacanthus septentrionalis (male); selecting flowers which are about to bloom in the first 20 days of April, removing petals at about 10 in the morning, leaving pistil only, pollinating and bagging; removing bags after 7 to 10 days, harvesting fruits when the fruits are yellowed in the middle ten-days of November, and sowing by taking pure peat soil as a sowing matrix in a greenhouse in the middle ten-days of December, watering fully before sowing, and covering a film and keeping moisture until seedlings emerge; after the seedlings grow to form 5 to 6 leaves, transplanting to a 72-mesh tray, recovering the seedlings, pinching once, applying 0.2 percent of Huaduoduo balance fertilizer once every 10 to 15 days; and planting in a nutritional bag after 3 to 4 months, and managing conventionally.

The invention relates to a rhododendron hybridum petal RhCHS gene promoter and flower color breeding application. According to the invention, a promoter with relatively high promoter activity is obtained from petal tissue of rhododendron hybridum. The full length of the promoter is 1477bp, the expression of a Chalcone synthase (CHS) gene can be efficiently started, the promoter disclosed by the invention can replace a promoter of the CHS gene with a low expression level in other species by virtue of a gene recombination technology, the enzyme activity of a CHS is improved, and the synthesis of anthocyanidin and anthocyanin in a downstream pathway of an anthocyanin metabolism pathway is increased, so that the color performance of petals is influenced. The promoter plays an important role in promoting breeding research on a plurality of horticultural flowers and plants including rhododendron and has good economic and social values.

A traditional Chinese medicine composition having blood activating and speckles removing effects and for treating butterfly rash and chloasma comprises herbs for oral administration: Radix et Rhizoma Rhodiolae 10-20 parts, Radix Angelicae Sinensis 10-20 parts, Folium Rhododendri Simsii 10-20 parts, Rhizoma Chuanxiong 10-20 parts, Radix Astragali 10-20 parts, Semen Persicae 10-20 parts, Flos Carthami 10-20 parts, Rhizoma Polygonati Odorati 10-20 parts, Fructus Gardeniae 10-20 parts, Radix Polygoni Multiflori Preparata 10-20 parts, Rhizoma Atractylodis Macrocephalae 10-20 parts, Radix Paeoniae Rubra 10-20 parts, Fructus Mori 10-20 parts, Herba Leonuri 10-20 parts, Semen Plantaginis 10-20 parts, Fructus Ligustri Lucidi 10-20 parts, Radix Aucklandiae 10-20 parts, Radix Bupleuri 10-20 parts, Margarita 10-20 parts, Ramulus Cinnamomi 10-20 parts, Flos Albiziae 10-20 parts, and Herba Artemisiae Anomalae 10-20 parts. The composition is effective in activating blood and promoting qi circulation and removing speckles and acnes, is applicable to the treatment of chloasma and butterfly rash caused by qi and blood stasis and skin nutrition loss, and can remove the chloasma and butterfly rash by internal regulation and external nourishing.

The invention discloses technology for promoting forming and rooting of rhododendron fortunei callus. The technology includes: laying a seedbed; collecting a cutting; making a cutting; inducing rooting; performing cuttage and management. A geothermal line is adopted to heat the seedbed during rooting, an automatic intermittent spraying system moisturizes, air temperature is controlled at 15.0-20.0 DEG C, relative humidity is 85.0-95.0%, and a bactericide is sprayed every other 14d to reduce rotting of the cutting. By the technology, the problem that rhododendron fortunei is difficult in cuttage propagation rooting, low in rooting rate and small in number of roots are solved effectively, rooting rate is up to 73.67%, and root systems are strong; the technology is an effective propagation method for rhododendron fortunei and is of important significance in accelerating development of rhododendron industrialization in China.

The invention relates to the field of traditional Chinese medicines, and in particular relates to an anti-cancer traditional Chinese medicinal composition, a preparation method and application thereof. The anti-cancer traditional Chinese medicinal composition consists of the following raw materials in parts by weight: 15-20 parts of dahurian rhododendron, 3-5 parts of gecko, 10-20 parts of rhizoma menispermi and 12-20 parts of fiveleaf akebia fruit, wherein the dahurian rhododendron is mixture of stems and leaves, the contents of female dahurian rhododendron and male dahurian rhododendron are the same in parts by weight. According to the anti-cancer traditional Chinese medicinal composition, the preparation method and application of the anti-cancer traditional Chinese medicinal composition in preparing anti-cancer medicines, the anti-cancer traditional Chinese medicinal composition acts on cancer cells by strengthening vital energy, eliminating stagnation and removing food retention, activating blood circulation, dredging channels and collaterals and the like through the synergic action of bulk traditional Chinese drugs in the prescription, so that growth and deterioration of early cancer cells can be effectively controlled, relapse or metastasis of cancer cells can be effectively controlled through surgical therapy, radiotherapy or chemical therapy, so that the effect of rescuing a patient or prolonging the life of the patient can be achieved.

The invention discloses an rhododendron phenylalanine ammonialyase PAL protein and a coding gene thereof. The rhododendron phenylalanine ammonialyase PAL protein comprises an amino acid sequence in a sequence table and a coding gene thereof; the gene is expressed in leaves, stamens, pistils and petals, and the expression in the leaves is obviously higher than that in other tissues; and in different development stages, the expression quantity in the blooming stage is the maximum. The invention provides a theoretical basis for improving plant quality and obtaining plants with different flower fragrances by using a genetic engineering technology in the future, makes up the blank in the related fields of rhododendron research at present, and has application value.

The invention provides a preparation method of rhododendron pulchrum anthocyanin microcapsules, and a product and application of the rhododendron pulchrum anthocyanin microcapsules. The method comprises the following steps: weighing a certain amount of prepared rhododendron pulchrum anthocyanin powder, and dissolving the rhododendron pulchrum anthocyanin powder in a buffer solution with a pH value of 1.5-3.5; adding monoglyceride into the rhododendron pulchrum anthocyanin solution, carrying out stirring and dissolving in a constant-temperature water bath at the temperature of 40 DEG C to 60 DEG C, and carrying out homogenizing at a high speed to form a primary emulsion; then, respectively putting Arabic gum and whey protein isolate into a buffer solution with the pH of 1.5-3.5 at the temperature of 40 DEG C to 60 DEG C, carrying out stirring and dissolving, and after two wall materials are completely dissolved, mixing the solution of the Arabic gum and the solution of the whey protein isolate; and finally, adding the primary emulsion, carrying out stirring, carrying out homogenizing at a high speed to form a compound emulsion, and carrying out spray drying, thereby obtaining the rhododendron pulchrum anthocyanin microcapsule. In the method, a mass ratio of the wall materials whey protein and the Arabic gum to the core material rhododendron pulchrum anthocyanin is 4: 1; and in a spray drying process, the temperature of an air inlet is 165 DEG C, and the feeding flow rate is 500mL/h. According to the preparation method, the original color and physiological activity of the rhododendron pulchrum anthocyanin can be effectively protected, the stability of the anthocyanin is improved, and the storage time is prolonged.

The invention discloses a making process of rhododendron black tea. The process comprises the following operation steps of picking, withering, rolling, sterilizing infectious microbes, fermenting, steaming tea leaves, aroma enhancement, pre-drying tea leaves, and baking the tea leaves to fix aroma. The improvement is that sterilization of infectious microbes before fermentation ensures rapid growth of kombucha in the fermentation process, and after fermentation, steaming of tea leaves ensures that fermentation is terminated in time and lipid aroma precursor substances are converted more on one hand, and on the other hand, formed amino acids and soluble sugar components are reserved as much as possible; after the tea leaves are steamed, then the tea leaves are immediately subjected to aroma enhancement by adopting a freeze-drying process, so that the rhododendron aroma is more naturally combined with the tea leaves, the aroma is natural and lasting, and the black and oily strip shape of the black tea is not damaged to the greatest extent. The prepared black tea is good in quality, black and oily in appearance, natural and lasting in fragrance, has the unique aroma of rhododendron, red, uniform and bright in soup color, mellow in taste and sweet in aftertaste, and the variety and content of aroma components are remarkably increased.

The invention provides a traditional Chinese medicine composition for treating rhinitis, and belongs to the technical field of medicine. The traditional Chinese medicine composition is prepared from the main raw materials of fructus aristolochiae, saccharum sinense Roxb., water chestnuts, caryopteris terniflora Maxim., fistular onion stalks, common ducksmeat herb, semen sterculiae lychnophorae, agilawood, juice of Chinese toona, yellow croaker ear-stone, leaves of rhododendron simsii Planch., centaurium pulchellum (Swartz) Druce var. altaicum Moench, ludwigia octovalvis (Jacq.) Raven, radix chimonanthus, common squill bulb, herb of henbit deadnettle and borneol. Besides, pharmaceutically acceptable auxiliary materials can be added into the traditional Chinese medicine composition, and the traditional Chinese medicine composition can be prepared into different dosage forms of medicine for treating rhinitis according to a conventional preparation method. Raw medicinal materials are carefully selected for the traditional Chinese medicine composition, the formula is reasonable, active ingredients in the traditional Chinese medicine composition have a mutual and synergistic effect and jointly perform the functions of ventilating the lung and relieving the stuffy nose, clearing away heat and toxic materials, relieving swelling and pain, refreshing the brain and the like, and the traditional Chinese medicine composition is significant in effect, capable of taking effect fast, short in treatment course, high in cure rate, capable of treating both symptoms and root causes and consolidated in treatment effect when used for treating rhinitis. Moreover, the traditional Chinese medicine composition is prepared from pure natural Chinese herb raw materials, so that the traditional Chinese medicine composition is mild, stable and lasting in acting efficacy, safe, reliable and free of toxic or side effects.

The invention discloses a preparation method of a health wine. The preparation method comprises the steps of weighing the following traditional Chinese medicinal materials by weight: 45g of sword bean roots, 10g of aeschynomene indica, 10g of peruvian groundcherry herbs, 7g of rhododendron simsii roots, 7g of leucas zeylanica, 7g of broad bean stems, 7g of white poplar barks and 3g of Yunnan alstonia roots, then, mixing and crushing to form coarse powder for later use; tightly wrapping the coarse powder by using gauze to form a traditional Chinese medicine bag, placing the traditional Chinese medicine bag into a vessel, adding 2000ml of white spirit with the alcohol content of 50 to soak, and then, placing the vessel at a shady, cool and dry place for one year after sealing the vessel; and finally, discarding the traditional Chinese medicine bag to obtain the health wine. Compared with the prior art, the health wine is prepared by taking pure traditional Chinese medicines as raw materials and organically matching and combining various traditional Chinese medicines so as to be easily absorbed by a human body and have the effects of invigorating qi and blood, nourishing and protecting health, enhancing immunity, improving sleep quality, promoting appetite and resisting to fatigue; the preparation method is simple; and the health wine is convenient to take, safe, free of toxic or side effect and good in taste.