Efficient direct somatic embryogenesis method of rhododendron fortunei

A technology of embryogenesis and somatic cells, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of slow propagation time and easy variation of cuttings, and solve the problems of cutting rooting difficulties, stable traits, and shortened group size. The effect of the training cycle

Active Publication Date: 2020-03-24
MINJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-efficiency direct somatic embryogenesis method of Rhododendron versicolor in order to solve the problem that the existing seed propagation and cutting propagation time of Rhododendron versicolor are slow and prone to variation

Method used

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  • Efficient direct somatic embryogenesis method of rhododendron fortunei
  • Efficient direct somatic embryogenesis method of rhododendron fortunei
  • Efficient direct somatic embryogenesis method of rhododendron fortunei

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Effect test

Embodiment 1

[0025] A method for direct somatic embryogenesis of rhododendron versicolor with high efficiency, comprising the following steps:

[0026] (1) Preparation of aseptic sowing seedlings of Rhododendron yunnanensis: the seeds of Rhododendron yunnanensis are sterilized and sowed in the culture medium at a temperature of 25°C and a light intensity of 80mol.m -2 .s -1 Cultivate for 3 months in the tissue culture room of the plant, obtain the aseptic sowing seedling of Rhododendron brocade for stand-by;

[0027] (2) Induction of leaf somatic embryos: In an ultra-clean workbench, take two young leaves from the top of Rhododendron yunnanensis seedlings prepared in step (1), place them on moist sterile filter paper, cut off both ends of the leaves with a blade, and remove the leaves from the front. Facing up, place the leaves flat on the surface of the prepared somatic embryogenesis induction medium, place four leaves on each plate, seal with parafilm and culture in the dark for 2 weeks...

Embodiment 2

[0039] A method for direct somatic embryogenesis of rhododendron versicolor with high efficiency, comprising the following steps:

[0040] (1) Preparation of aseptic sowing seedlings of Rhododendron yunnanensis: the seeds of Rhododendron yunnanensis are sterilized and sowed in the culture medium at a temperature of 25°C and a light intensity of 80mol.m -2 .s -1 Cultivate for 2.5 months in the tissue culture room of the same plant, and obtain the aseptic seeding seedlings of Rhododendron brocade for stand-by;

[0041] (2) Induction of leaf somatic embryos: In an ultra-clean workbench, take 4 young leaves from the top of Rhododendron yunnanensis seedlings prepared in step (1), place them on moist sterile filter paper, cut off both ends of the leaves with a blade, and cut off the front Facing up, place the leaves flat on the surface of the prepared somatic embryogenesis induction medium, place four leaves on each plate, seal with parafilm and culture in the dark for 2 weeks at 2...

Embodiment 3

[0048] A method for direct somatic embryogenesis of rhododendron versicolor with high efficiency, comprising the following steps:

[0049] (1) Preparation of aseptic sowing seedlings of Rhododendron yunnanensis: the seeds of Rhododendron yunnanensis are sterilized and sowed in the culture medium at a temperature of 25°C and a light intensity of 80mol.m -2 .s -1 Cultivate for 3 months in the tissue culture room of the plant, obtain the aseptic sowing seedling of Rhododendron brocade for stand-by;

[0050] (2) Induction of leaf somatic embryos: In an ultra-clean workbench, take 3 young leaves from the top of Rhododendron yunnanensis seedlings prepared in step (1), place them on moist sterile filter paper, cut off both ends of the leaves with a blade, and cut off the front Facing up, place the leaves flat on the surface of the prepared somatic embryogenesis induction medium, place four leaves on each plate, seal with parafilm and culture in the dark for 2 weeks at 25°C; then place...

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Abstract

The invention discloses an efficient direct somatic embryogenesis method of rhododendron fortunei, and belongs to the technical field of rapid propagation and regeneration of plants. An efficient somatic embryogenesis system of rhododendron fortunei is established for the first time by the efficient direct somatic embryogenesis method of the rhododendron fortunei. In the efficient somatic embryogenesis system of the rhododendron fortunei, seedling leaves of the rhododendron fortunei are used as explants of direct somatic embryogenesis for generating plants; and thus, the direct somatic embryogenesis method of the rhododendron fortunei is an efficient asexual breeding way to obtain a large number of tissue-cultured rhododendron fortunei seedlings. The efficient direct somatic embryogenesismethod of the rhododendron fortunei improves rapid tissue-culture breeding systems of Sect. Ponticum G. Don, and solves the problems of long time consumption, liable variation and extremely low cuttage propagation coefficient of conventional sowing methods. The efficient direct somatic embryogenesis method of the rhododendron fortunei is capable of rapidly breeding a large number of plants with stable characters, thereby rapidly establishing clones of fine individual plants; and thus, the method has important application values in the aspects of seedling production, germplasm innovation and new breed selection of the rhododendron fortunei seedlings.

Description

technical field [0001] The invention belongs to the technical field of rapid plant reproduction and regeneration, and in particular relates to a high-efficiency direct somatic cell embryogenesis method of rhododendron versicolor. Background technique [0002] As an important parent of Rhododendronaceae, Rhododendron yunjin has high ornamental value, is extremely cold-resistant and has fragrance. The traditional propagation method is seed sowing or stem cutting, but the breeding cycle is long and the efficiency is low, so the regeneration of plants through in vitro culture is an efficient and rapid method for mass propagation of plants. Rhododendron versicolor is a woody plant, and the plant contains more polyphenols that will hinder cell division, so the establishment of its regeneration system is relatively difficult, and the regeneration scheme through somatic embryogenesis has not yet been established. [0003] Embryogenic cells have a strong ability to accept foreign DN...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 魏翔莺张春英郑秀霞穆景利
Owner MINJIANG UNIV
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