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30results about How to "Seedling speed is fast" patented technology

Fast efficient propagation method of Paris polyphylla

InactiveCN102870564ASeedling speed is fastMore germinationHorticultureBudRhizome
The invention discloses a fast efficient propagation method of Paris polyphylla. The fast efficient propagation method of Paris polyphylla includes: selecting and treating propagating materials; inducing latent buds to germinate by stratification; and managing seedling propagation and production. The fast efficient propagation method of Paris polyphylla has the advantages that seedling growth is fast, propagation coefficient is high, and seedling quality is high. The latent buds are induced to germinate by inhibiting apical dominance without damaging completeness of rhizomes, stem cutting for seedling separation is performed according to germinating latent bud spots, and accordingly many latent buds germinate, and many separate seedlings are grown. Seedling rate is one to two times higher than that in the traditional way that segmental cutting is performed prior to inducing the latent buds to germinate. When the latent buds are induced to germinate, the rhizomes are not damaged. Therefore, seedlings from the latent buds are robust. By sterilization and seed soaking prior to seedling raising after cutting and seedling separation, strong seedlings are easy to obtain and survive easily after planted in fields.
Owner:临沧道地中药材种植科技有限公司

Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings

The invention relates to hormone-free tissue culture and a rapid propagation method of anoectochilus formosanus seedlings. The method comprises the following four tissue culture stages: 1) seeds germin ate without bacteria to form protocorms; 2) the protocorms is propagated and differentiation culture is performed on plantlets; 3) strong seedlings of aseptic seedlings are cultured; 4) rooting culture is performed, and different hormone-free culture media are adopted at different tissue culture stages. The method provided by the invention has the advantages that the operation is simple and convenient, the process is less, the cost is low, frequent subculture is not required to be performed on tissue culture seedlings, exogenous plant hormones are not used during the whole culture process, the problems that a large number of exogenous plant hormones are used, the production cost is high, the seedlings are irregular, and the seedlings are unsafe to eat exist in the conventional process of culturing anoectochilus formosanus tissues are solved better, the quality and the transplanting survival rate of the seedlings are improved, and security of using the seedlings is improved.
Owner:福建省农业科学院农业生物资源研究所

Method for propagating Millettia speciosa Champ. seedlings using cottage

The invention discloses a method for propagating Millettia Speciosa germchit by cuttage, including first-phase preparation and later management: 1) during the first-phase preparation, Millettia Speciosa tresses are selected and cut into stem segments for spare use; river sand and coco bran are evenly mixed according to certain proportion to obtain a substrate for spare use; 2) during the later management, Millettia Speciosa stem segments are dipped with root stimulate at the base parts and then stuck into the substrate; the stem segments are watered to promote the close contact between the base parts thereof and the substrate; the stuck stem segments are shaded for culture, with the moisture of the substrate and air humidity being guaranteed; after taking root, the stem segments are fertilized with urea solution until the germchit grows up. The invention has the advantages of simple process flow and low manufacturing cost, and, effectively utilizes the scrap Millettia Speciosa tresses; by optimizing the root stimulate and the substrate formula, the invention improves the culture conditions and increases the survival rate of the stuck stem segments. The method has high feasibility, simple operation, low cost input and high survival rate and achieves the dual purpose of recycled utilization of resources and batch production of the germchit.
Owner:TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

Method for growing Paris polyphylla seedlings from seeds

The invention belongs to the technical field of Paris polyphylla seedling growing, and particularly relates to a method for growing Paris polyphylla seedlings from seeds. The method comprises the steps of preparation of a seed bed, treatment of the seeds, sowing and seedling growing, and seedling management. Through adoption of the method, defects of low germination rate and long seedling growing period in an existing Paris polyphylla seed propagation method are overcome. The method disclosed by the invention has a high seedling speed, and buds come out 15-30 days after sowing. The wounds of the seeds are disinfected by using mancozeb wettable powder liquid, and the seeds are soaked into a rooting powder solution, so that the germination rate of the seeds is increased greatly, and the reproduction coefficient is increased.
Owner:NINGGUO THOUSAND SQUARE TCM DEV

Method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in Changbai Mountains

The invention relates to plant propagation and in particular relates to a method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in the Changbai Mountains. Taking rhododendron aureum as example, the method comprises the steps of: (1) collecting branches of the rhododendron aureum in late October, carrying out low-temperature dormancy on the branches, carrying out water planting to promote the germination and the growth of lateral buds and cutting in a form of one segment with one leaf after the treatment to be used as explants for later use, wherein the culture medium for the rooting of the stem segments and the germination and the growth of the lateral buds comprises the components of: the basic culture medium+ 0.13-0.14mg.L-1 of KT (kinetin)+ 0.05-0.08mg.L-1 of IBA (Indole Butyric Acid)+ 0.02-0.05mg.L-1 of NAA (Naphthyl Acetic Acid)+ 2.70-2.90mg.L-1 of GA(Gibberellin)3. According to the method, the process is simple, the cost is low, the operability is strong and the capability of improving the seedling producing efficiency is high, so that the method can be directly applied to industrial seedling cultivation of the rhododendra.
Owner:TONGHUA NORMAL UNIV

Cultivation method of baccaurea ramiflora lour potted plant

The invention discloses a cultivation method of a baccaurea ramiflora lour potted plant. The cultivation method comprises the following steps of (1) selecting potted seedlings; (2) pretreating the potted seedlings; (3) performing potting; (4) performing potted plant management; (5) performing grafting propagation; (6) performing trimming and shaping; (7) performing daily maintenance; and (8) performing flower and fruit management. According to the cultivation method of the baccaurea ramiflora lour potted plant, the raw material ratio of a fertilizer is reasonable, enough nutritional ingredients can be provided for growth of baccaurea ramiflora lour, the seedling forming speed of baccaurea ramiflora lour seedlings is increased, and the yield of baccaurea ramiflora lour fruits and the quality of pulp are improved. Mature branches of the baccaurea ramiflora lour with high-quality germplasm are selected as scions, so that the potted plant after grafting is high in survival rate, high in stress resistance, early in fruiting and high in yield. After being trimmed and shaped, the baccaurea ramiflora lour potted plant is attractive in appearance and has high ornamental value. The cultivation method of the baccaurea ramiflora lour potted plant provides guarantee for large-scale potted plant cultivation, has a good market prospect, and is suitable for vast farmers to plant.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

Fast efficient propagation method of Paris polyphylla

InactiveCN102870564BSeedling speed is fastMore germinationHorticultureBudCell budding
The invention discloses a fast efficient propagation method of Paris polyphylla. The fast efficient propagation method of Paris polyphylla includes: selecting and treating propagating materials; inducing latent buds to germinate by stratification; and managing seedling propagation and production. The fast efficient propagation method of Paris polyphylla has the advantages that seedling growth is fast, propagation coefficient is high, and seedling quality is high. The latent buds are induced to germinate by inhibiting apical dominance without damaging completeness of rhizomes, stem cutting for seedling separation is performed according to germinating latent bud spots, and accordingly many latent buds germinate, and many separate seedlings are grown. Seedling rate is one to two times higher than that in the traditional way that segmental cutting is performed prior to inducing the latent buds to germinate. When the latent buds are induced to germinate, the rhizomes are not damaged. Therefore, seedlings from the latent buds are robust. By sterilization and seed soaking prior to seedling raising after cutting and seedling separation, strong seedlings are easy to obtain and survive easily after planted in fields.
Owner:临沧道地中药材种植科技有限公司

Method for breeding high-yield xanthoceras sorbifolia strain

The invention belongs to the technical field of new plant variety cultivation, and discloses a method for breeding a high-yield xanthoceras sorbifolia strain. The method for breeding the high-yield xanthoceras sorbifolia strain comprises the following steps: collecting seeds, breaking a dormancy period, identifying a male parent by adopting an SSR molecular marker after germination and emergence,and screening a high-quality mother plant; taking a xanthoceras sorbifolia container sapling as a stock, grafting a selected single plant scion, identifying a male parent for borne seeds, and performing second-generation grafting and male parent identification after confirming that the seeds are self-fruitful; if seeds are self-fruitful again, determining the mother plant as a final single plant;and taking the scion of the final single plant, taking a 3-5-year-old xanthoceras sorbifolia seedling as a stock, and performing grafting and expanding propagation to obtain a self-fertile high-yieldxanthoceras sorbifolia strain. The method is simple, convenient, trouble-saving, labor-saving and easy to operate; seeding is carried out after dormancy breaking, so that the field seedling rate is increased, and the seed germination and emergence speed is increased; and the problem of shortage of high-yield improved seeds of the xanthoceras sorbifolia is efficiently and rapidly solved, and the urgent need of current xanthoceras sorbifolia industry development is met.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Fir tree seedling culture method

The invention discloses a fir tree seedling culture method. The method includes the steps of seed pretreatment, sowing and seedling raising. A special seed dressing is adopted in the stage of seed pretreatment, the germination rate of seeds is improved, the speed of seed germination is speeded up, the seeds which are germinative are planted in a film container one by one for culture in a greenhouse, conditions such as seedling culture environment illumination, temperature and humidity are artificially controlled and are not subjected to the influence of the outside environment, the seedlings can be transported together with a film container after being grown, the seedlings do not need to be moved out of a culture medium, the seedling root systems are not damaged, and the method is more suitable for transplantation and greatly improves seedling transplanting survival rate.
Owner:NANJING LVZHICHENG PATENT TECH DEV CO LTD

Peach tree seedling culturing method

The invention relates to the technical field of peach tree seedling culture, particularly a peach tree seedling culture method. Wild peach or Carya cathayensis seeds are soaked in a nutrient solution,so that the activity is enhanced, the bud ratio in the rootstock culture process is higher, the waste of wild peach or Carya cathayensis seeds is lowered, the quick growth of the rootstock is promoted, and the seedling growth speed of the rootstock is increased. By combining the selection of peach tree continuous cropping branches and the cuttage culture of scions, the scions have higher diseaseresistance and higher survival rate of grafting; and in the rootstock and scion culture process, the activities of the rootstock and scions are enhanced, the healing speed in the grafting process is increased, and the survival rate is enhanced.
Owner:贵州宏海丰林林业科技发展有限公司

Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings

The invention relates to hormone-free tissue culture and a rapid propagation method of anoectochilus formosanus seedlings. The method comprises the following four tissue culture stages: 1) seeds germin ate without bacteria to form protocorms; 2) the protocorms is propagated and differentiation culture is performed on plantlets; 3) strong seedlings of aseptic seedlings are cultured; 4) rooting culture is performed, and different hormone-free culture media are adopted at different tissue culture stages. The method provided by the invention has the advantages that the operation is simple and convenient, the process is less, the cost is low, frequent subculture is not required to be performed on tissue culture seedlings, exogenous plant hormones are not used during the whole culture process, the problems that a large number of exogenous plant hormones are used, the production cost is high, the seedlings are irregular, and the seedlings are unsafe to eat exist in the conventional process of culturing anoectochilus formosanus tissues are solved better, the quality and the transplanting survival rate of the seedlings are improved, and security of using the seedlings is improved.
Owner:福建省农业科学院农业生物资源研究所

Method for raising Qilian juniper seedlings by carrying out cuttage by virtue of twigs

The invention relates to a method for raising Qilian juniper seedlings by carrying out cuttage by virtue of twigs. The method comprises the following steps: (1) arranging a slotting machine and treating a cutting medium, namely arranging the cutting medium in the slotting machine, sterilizing soil firstly before the cuttage, covering the upper part of the soil with a sunshade net, placing for 1 day, and then carrying out cuttage; (2) collecting cutting slips, namely cutting the cutting slips from a mother plant by adopting a blade, then aligning the bases of the cutting slips, and immersing the bases into clear water to be treated later; (3) carrying out pre-treatment on the cutting slips, namely immersing the cutting slips into treatment solution, and carrying out cuttage while treatment is carried out; and (4) carrying out hormone soaking, namely carrying out rooting powder ABT treatment on each cutting slip for 200-300 minutes while concentration is 10-200mg / l, and carrying out acceptance 60 days later. The method provided by the invention has the advantages that Qilian juniper seedling development speed is greatly improved, the technical problems that Qilian juniper seed regeneration is difficult, growth and development of seedlings are poor, distribution is sparse, short and twist are caused and seedling development speed is low in the past are solved, and the method provided by the invention has an important role on large-scale industrial production and propagation of Qilian juniper plants.
Owner:GANSU DESERT CONTROL RES INST

Cultivation method for regeneration seedlings of hybrid of populus simonii and populus euphratica

The invention discloses a cultivation method for regeneration seedlings of a hybrid of populus simonii and populus euphratica. The cultivation method is characterized by comprising the following steps: establishment of an explant sterilizing and sterility system, adventitious bud induction and proliferation, rooting culture and acclimatization and transplantation. According to the invention, by adopting a tissue culturing method, rapid propagation of populus simonii and populus euphratica high-quality test-tube plantlet is realized within relatively short time, and only about 60 days are needed from cluster buds induction and proliferation to test-tube plantlet rooting in the whole propagation process, and thus greatly increasing the plantlet formation speed of populus simonii and populuseuphratica, having important functions for the large-scale and industrialized production and propagation of plants of populus simonii and populus euphratica, and continuously supplying high-quality nursery stocks for saline land reclamation of the dry desert region and afforestation in the desert region in the northwest of China.
Owner:GANSU DESERT CONTROL RES INST

Layering method of Uganda Warburgia

The invention discloses a layering method of Uganda Warburgia. The layering method of the Uganda Warburgia includes steps: 1) under the condition that air temperature ranges from 15 degree centigrade to 35 degree centigrade, selecting a first grade branch of a Uganda Warburgia plant straight and upright in branch shape, and free of disease and insect attack, which is close to the ground, and girdling the middle upper portion of the first grade branch of the Uganda Warburgia plant; 2) burying the girdled portion of the first grade branch into a position of soil, 10-15cm in depth, fixing the first grade branch in the soil, exposing the head of the first grade branch in air, and then pouring a rooting powder solution after being diluted with water by 1000-2000 times on the girdled portion of the first grade branch, and afterwards pouring rooting powder liquid of the same concentration as the rooting powder solution on the girdled portion of the first grade branch every other 2-3 days, totally pouring the rooting powder liquid on the girdled portion of the first grade branch for 5-10 times, and keeping water content of the position in the of soil, where the first grade branch is buried, at 70%-80%; 3) generating a new root on the girdled portion of the first grade branch after 90-120days, enabling the new root on the girdled portion of the first grade branch to develop well after 5-6 months, separating the first grade branch with the root developed well from the Uganda Warburgia seed tree, and then digging the first grade branch with the root developed well out with the soil, and finally transplanting the first grade branch. The layering method of the Uganda Warburgia is simple, strong in maneuverability, quick in reproduction speed, and high in survival percent.
Owner:WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI

Semi-hydroponics cutting rooting method for lyceum ruthenicm murr

The invention provides a semi-hydroponics cutting rooting method for lyceum ruthenicm murr, belonging to the technical field of cutting of lyceum ruthenicm murr. The method comprises the following steps of (1) oscillating twigs of lyceum ruthenicm murr for 7-9h at 26-30 DEG C, treating the oscillated twigs for 0.5-1.5h at low temperature of 6-9 DEG C so as to obtain to-be-plant twigs; (2) inserting the to-be-plant twigs into a matrix for rooting for 40-50d so as to obtain field transplanted seedlings, wherein a rooting solution is irrigated into the matrix in rooting process, and the mass to volume ratio (g / ml) of the matrix to the rooting solution is kept at 1:(2-3). Through adoption of the method, the rooting rate of the planted twigs of lyceum ruthenicm murr can reach 100%, the roots grow strongly, and 40-50d later, field transplanted seedlings can be obtained; and the method is easy to operate, the seedling culture period is short, the seedlings growing speed is high, so that the method can be applied to industrial cultivation of lyceum ruthenicm murr seedlings.
Owner:GANSU DESERT CONTROL RES INST

Lycium ruthenicum tissue culture adventitious bud ex-vitro rooting method

The invention provides a lycium ruthenicumtissue culture adventitious bud ex-vitro rooting method, which belongs to the technical field of lycium ruthenicum tissue culture. The method comprises the following steps of (1) culturing lycium ruthenicum stem sections into an adventitious bud culture medium to obtain an adventitious bud; (2) directly hardening the adventitious bud obtained in the adventitious bud culture medium to obtain an adventitious bud with a root primordium; feeding a rooting powder solution into the adventitious bud culture medium during a seedling hardening process; (3) sterilizing the adventitious bud with the root primordium, soaking a root part through an IBA solution, transplanting into a substrate, and culturing to obtain a lycium ruthenicum grown-up seedling. The method is simple and convenient to operate and low in culture cost; the whole production cost is reduced by not less than 20 percent; the method is more suitable for mass industrialized production andvariety breeding of lycium ruthenicum seedlings, lays a solid foundation for building a lycium ruthenicum asexual rapid propagation system and providing seedlings for ecological environment construction in northwest China, and has better economic benefit, social benefit and ecological benefit.
Owner:GANSU DESERT CONTROL RES INST

Codonopsis pilosula protected field substrate seedling raising method

The invention belongs to the field of codonopsis pilosula seedling production, and particularly relates to a codonopsis pilosula protected area matrix seedling raising method. Aiming at the problems of long land occupation time, poor seedling quality and the like of traditional codonopsis pilosula seedling culture, the method is carried out in a sunlight greenhouse, reasonable environmental conditions can be provided for codonopsis pilosula seedlings through soil preparation, seed pretreatment, selection of sowing time and sowing quantity, sowing, field management and timely digging and transplanting, anti-season codonopsis pilosula seedling culture is realized, and the survival rate of codonopsis pilosula seedlings is increased. The seedling quality is improved.
Owner:沁源县利农生物科技有限公司

Method for propagating Millettia speciosa Champ. seedlings using cottage

The invention discloses a method for propagating Millettia Speciosa germchit by cuttage, including first-phase preparation and later management: 1) during the first-phase preparation, Millettia Speciosa tresses are selected and cut into stem segments for spare use; river sand and coco bran are evenly mixed according to certain proportion to obtain a substrate for spare use; 2) during the later management, Millettia Speciosa stem segments are dipped with root stimulate at the base parts and then stuck into the substrate; the stem segments are watered to promote the close contact between the base parts thereof and the substrate; the stuck stem segments are shaded for culture, with the moisture of the substrate and air humidity being guaranteed; after taking root, the stem segments are fertilized with urea solution until the germchit grows up. The invention has the advantages of simple process flow and low manufacturing cost, and, effectively utilizes the scrap Millettia Speciosa tresses; by optimizing the root stimulate and the substrate formula, the invention improves the culture conditions and increases the survival rate of the stuck stem segments. The method has high feasibility,simple operation, low cost input and high survival rate and achieves the dual purpose of recycled utilization of resources and batch production of the germchit.
Owner:TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

A kind of layering method of tens of camphor in Uganda

The invention discloses a layering method of Uganda Warburgia. The layering method of the Uganda Warburgia includes steps: 1) under the condition that air temperature ranges from 15 degree centigrade to 35 degree centigrade, selecting a first grade branch of a Uganda Warburgia plant straight and upright in branch shape, and free of disease and insect attack, which is close to the ground, and girdling the middle upper portion of the first grade branch of the Uganda Warburgia plant; 2) burying the girdled portion of the first grade branch into a position of soil, 10-15cm in depth, fixing the first grade branch in the soil, exposing the head of the first grade branch in air, and then pouring a rooting powder solution after being diluted with water by 1000-2000 times on the girdled portion of the first grade branch, and afterwards pouring rooting powder liquid of the same concentration as the rooting powder solution on the girdled portion of the first grade branch every other 2-3 days, totally pouring the rooting powder liquid on the girdled portion of the first grade branch for 5-10 times, and keeping water content of the position in the of soil, where the first grade branch is buried, at 70%-80%; 3) generating a new root on the girdled portion of the first grade branch after 90-120days, enabling the new root on the girdled portion of the first grade branch to develop well after 5-6 months, separating the first grade branch with the root developed well from the Uganda Warburgia seed tree, and then digging the first grade branch with the root developed well out with the soil, and finally transplanting the first grade branch. The layering method of the Uganda Warburgia is simple, strong in maneuverability, quick in reproduction speed, and high in survival percent.
Owner:WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI

Method for high-quality chuzhou chrysanthemum hole tray cuttage seedling culture

The invention discloses a method for high-quality dicotyledoneae hole tray cuttage seedling culture. The method includes cutting out chuzhou chrysanthemum fringe strips from detoxified chuzhou chrysanthemum mother plants, immersing the fringe strips through a mixed solution of a rooting regeant and a fungicide, inserting the processed fringe strips into a hole tray matrix, maintaining humidity of the hole cavity matrix to be 80%, keeping growth temperature not higher than 35 DEG C and controlling the matrix humidity to be maintained around 60% when the chrysanthemum root hair is 2-4 cm. The chrysanthemum seedling root system can meet requirements for transplanting, and seedling output can be achieved. Optimum environment of seedling growth is manually created, seedling growing quality of the chuzhou chrysanthemum can be ensured, and the method ensures that the chuzhou chrysanthemum can be supplied in all weather without limit of seasons. The method adopts soil-free matrix, can easily control pest and disease damage, is high in seedling growth speed and high in garden-out rate. The method prolongs seedling supply period, expands application range of the chuzhou chrysanthemum, is capable of promoting production and supply of specialized chuzhou chrysanthemum seedlings, promotes specialized and standardized production of chuzhou chrysanthemum in location regions and enables traditional famed flower industry to be further developed.
Owner:ANHUI JUTAI CHUJU HERBAGE TECH

Seedling hardening method for iris tissue-cultured seedlings

The invention provides a seedling hardening method for iris tissue-cultured seedlings. The seedling hardening method comprises the following steps: cleaning roots of rooted iris tissue-cultured seedlings, carrying out sterilization, transplanting the iris tissue-cultured seedlings into a seedling-hardening matrix, and carrying out seedling hardening at a temperature of 10-13 DEG C and humidity of55%-85%, wherein the iris tissue-cultured seedlings are iris germanica tissue-cultured seedlings. According to the seedling hardening method provided by the invention, the temperature is limited at 10-13 DEG C, and meanwhile, the humidity is limited at 55%-85%, so that the seedling-hardening survival rate of the iris germanica tissue-cultured seedlings can be remarkably increased, and the cost islowered.
Owner:INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD +1

Method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in Changbai Mountains

The invention relates to plant propagation and in particular relates to a method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in the Changbai Mountains. Taking rhododendron aureum as example, the method comprises the steps of: (1) collecting branches of the rhododendron aureum in late October, carrying out low-temperature dormancy on the branches, carrying out water planting to promote the germination and the growth of lateral buds and cutting in a form of one segment with one leaf after the treatment to be used as explants for later use, wherein the culture medium for the rooting of the stem segments and the germination and the growth of the lateral buds comprises the components of: the basic culture medium+ 0.13-0.14mg.L-1 of KT (kinetin)+ 0.05-0.08mg.L-1 of IBA (Indole Butyric Acid)+ 0.02-0.05mg.L-1 of NAA (Naphthyl Acetic Acid)+ 2.70-2.90mg.L-1 of GA(Gibberellin)3. According to the method, the process is simple, the cost is low, the operability is strong and the capability of improving the seedling producing efficiency is high, so that the method can be directly applied to industrial seedling cultivation of the rhododendra.
Owner:TONGHUA NORMAL UNIV

A method for rapid propagation of medicinal plant Lycium barbarum black fruit in desert area

The invention discloses a quick propagation method for medicinal plant lycium ruthenicum in deserts. The quick propagation method comprises the following step 1, culturing of sterile seedlings (MS (Murashige) culture medium); 2, inducing of curative tissues (MS + 0.3mg / L of 6-benzylamino adenine + 1.0mg / L of 2,4-dichlorphenoxyacetic acid); 3, multiplication culture of multiple shoots (MS + 0.3mg / L of 6-benzylamino adenine + 0.3mg / L of kinetin + 0.5mg / L of naphthylacetic acid); 4, rooting culturing (1 / 2 of MS + 0.5mg / L of naphthylacetic acid + 0.5mg / L of MgSO4), wherein the culturing conditions of each step are the same, the temperature is 24+ / -2 DEG C, the light intensity is 1500-2000lx, and the light duration is 14h / d. The quick propagation method has the beneficial effects that by adopting the tissue culturing method, under the condition of artificial control, the quick propagation of lycium ruthenicum seedlings is realized, and the duration from inducing of curative tissues to the seedling rooting is only 55d; compared with the traditional sexual propagation, the segregation of character of the plant in the growth process is avoided, the propagation speed is high, the cost is low, the survival rate is high, the seedling growing speed is high, and the like.
Owner:GANSU DESERT CONTROL RES INST

Tissue culture method of Syringa microphylla and method for rapidly obtaining large seedlings of Syringa microphyllum

This application relates to the tissue culture method of Lilac microphylla and the method for rapidly obtaining large seedlings of Lilac microphylla. The tissue culture method comprises the following steps: (1) selection and disinfection of explants: cutting the top part of the young shoots of the year, and cutting it into Have the stem section of terminal bud or 1-2 axillary bud as explant, carry out disinfection; Germination of terminal buds or axillary buds; the starting medium is based on MS medium, and 30g / L of sucrose, 6g / L of agar, 1mg / L of hormone BA, and 0.2mg / L of hormone NAA are added at the same time, and the pH is adjusted to 5.8-6.2 (3) subculture; (4) rooting culture; (5) transplanting to obtain tissue culture seedlings. The method makes the explant of the lilac lobata explants have a high survival rate during the initiation culture process, is not easy to produce callus tissue, and has a high induction rate of axillary buds or terminal buds.
Owner:内蒙古和盛生态科技研究院有限公司 +1

A method for growing wild soybeans on saline-alkali land

The invention provides a method of planting wild soybeans on saline-alkali soil. The method comprises the steps of preparing culture matrix soil for seedling culture: mixing grass carbon, common soil, perlite and vermiculite at a volume ratio of 3:3:2:2, adding 230-270g carbendazim and 4.5-5.5kg compound fertilizer into a mixture per cubic meter, and uniformly stirring the mixture to form the culture matrix soil for the seedling culture, and growing seedlings: performing the seedling culture on the wild soybeans with the culture matrix soil.
Owner:赵春

Propagation method for rapid seedling formation of actinidia arguta

The invention discloses a propagation method for rapid seedling formation of actinidia arguta. The propagation method comprises the following steps of 1, explant selection, specifically, in November every year, selecting a robust plant growing for 1-2 years, selecting a branch with the stem thickness of 0.5-0.8 cm and full axillary buds on the plant, and cutting the branch into cutting sections with the length of 15-20 cm, and enabling each section to be provided with 3-5 buds; 2, pretreatment, specifically, putting the cutting sections obtained in the step 1 into a K2SO4 solution with the concentration of 0.05-0.13 mg.L<-1>, and soaking for 30-40 minutes; and 3, induction culture, specifically, fishing out the cutting sections pretreated in the step 2, covering the cutting sections with wet cloth with the thickness of 3-5 mm, culturing in an environment with the temperature of 20-32 DEG C and the humidity of 60%-75%, and washing the cutting sections with water with the temperature of 25 DEG C once every morning and evening in the period to obtain rooting and sprouting cutting sections. The method is low in cost, efficient, rapid, simple and high in operability, and can be widely applied to rapid large-scale seedling propagation cultivation of the actinidia arguta.
Owner:LUDONG UNIVERSITY

A kind of cultivation method of wood milk fruit potted plant

The invention discloses a method for cultivating potted plants of cassava, the cultivating process of which is as follows: (1) selection of potted seedlings; (2) pretreatment of potted seedlings; (3) potting; (4) potted plant management; (5) ) grafting; (6) pruning and shaping; (7) daily maintenance; (8) flower and fruit management. The raw material ratio of the fertilizer of the method for cultivating the cacao fruit potted plant of the present invention is reasonable, can provide sufficient nutritional ingredients for the growth of the cacao fruit, accelerate the seedling growth rate of the cacao fruit seedlings, and improve the output and production efficiency of the cacao fruit. The quality of the pulp. In addition, the mature branches of cassava fruit with excellent germplasm are used as scions, and the potted plants after grafting have high survival rate, strong stress resistance, early knot and high yield. After pruning and shaping, the potted plant of cayenne milk fruit has a beautiful shape and has a high ornamental value. The method for cultivating the cacao potted plant of the present invention provides guarantee for the large-scale potted cultivation of cacao cacao, has a good market prospect, and is suitable for planting by farmers.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

A kind of rooting method of adventitious buds outside the bottle of Tissue Culture of Lycium ruthenicum

The invention provides a lycium ruthenicumtissue culture adventitious bud ex-vitro rooting method, which belongs to the technical field of lycium ruthenicum tissue culture. The method comprises the following steps of (1) culturing lycium ruthenicum stem sections into an adventitious bud culture medium to obtain an adventitious bud; (2) directly hardening the adventitious bud obtained in the adventitious bud culture medium to obtain an adventitious bud with a root primordium; feeding a rooting powder solution into the adventitious bud culture medium during a seedling hardening process; (3) sterilizing the adventitious bud with the root primordium, soaking a root part through an IBA solution, transplanting into a substrate, and culturing to obtain a lycium ruthenicum grown-up seedling. The method is simple and convenient to operate and low in culture cost; the whole production cost is reduced by not less than 20 percent; the method is more suitable for mass industrialized production andvariety breeding of lycium ruthenicum seedlings, lays a solid foundation for building a lycium ruthenicum asexual rapid propagation system and providing seedlings for ecological environment construction in northwest China, and has better economic benefit, social benefit and ecological benefit.
Owner:GANSU DESERT CONTROL RES INST
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