Method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in Changbai Mountains

Abstract

The invention relates to plant propagation and in particular relates to a method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in the Changbai Mountains. Taking rhododendron aureum as example, the method comprises the steps of: (1) collecting branches of the rhododendron aureum in late October, carrying out low-temperature dormancy on the branches, carrying out water planting to promote the germination and the growth of lateral buds and cutting in a form of one segment with one leaf after the treatment to be used as explants for later use, wherein the culture medium for the rooting of the stem segments and the germination and the growth of the lateral buds comprises the components of: the basic culture medium+ 0.13-0.14mg.L-1 of KT (kinetin)+ 0.05-0.08mg.L-1 of IBA (Indole Butyric Acid)+ 0.02-0.05mg.L-1 of NAA (Naphthyl Acetic Acid)+ 2.70-2.90mg.L-1 of GA(Gibberellin)3. According to the method, the process is simple, the cost is low, the operability is strong and the capability of improving the seedling producing efficiency is high, so that the method can be directly applied to industrial seedling cultivation of the rhododendra.

Description

[0001] technical field [0002] The invention relates to a method for high-efficiency seedling growth in test tubes using the stems of rhododendrons in Changbai Mountains. Background technique [0003] In the prior art, Rhododendron plants in Changbai Mountains include Rhododendron cowhide ( Rhododendron chrysanthum Pall.), short-fruited cuckoo ( Rhododendron brachycarpum D. Don), Zhaobai Rhododendron ( Rhododendron micranthum Turcz.), Rhododendron microphylla ( Rhododendron parvifolium Adams.), Rhododendron ( Rhododendron schlippenbachii Maxim.), Xing'an Rhododendron ( Rhododendron dauricum L.), Red Rhododendron ( Rhododendron mucronulatum Turcz.), felt cuckoo ( Rhododendron confertissimum Nakai) and rhododendron ( Rhododendron redowskianum Maxim.) and so on have a total of 9 species, all of which are key protected plants in Jilin Province. Among them, Rhododendron spp. Rhododendron , Rhododendron zhaobai and Rhododendron small-leaf are evergreen shrub...

AI Technical Summary

Problems solved by technology

These nine Rhododendron species have small seeds, low germination rate, and extremely low survival rate of seedlings formed after germination; cutting propagation requires a large number of wild Rhododendron branches, which greatly damages wild resources, and the rooting rate and transplanting survival rate are extremely low. and use is severely limited

[0005] At present, there are many reports on the in vitro propagation of 9 species of Rhododendron, which have the advantages of high proliferation coefficient through callus redifferentiation sprouts, axillary bud clusters and direct regeneration, but there are still explant-induced callus redifferentiation and axillary bud regeneration. The cycle of clustering and direct regeneration is long, the speed of obtaining adventitious buds and further rooting is slow, the steps are complicated, the cost is high, and the operability is poor. Long-term continuous proliferation is prone to degradation and variation.

Images