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Propagation method for rapid seedling formation of actinidia arguta

A rapid technology of Actinidia jujube, which is applied in the field of rapid seedling growth of Actinidia jujube, can solve the problems of poor operability, high input cost, variation or mutation, etc., and achieve the goal of enhancing rooting, increasing seedling speed, and improving antibacterial properties Effect

Pending Publication Date: 2021-04-09
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue culture not only has high investment cost and poor operability, but also may cause variation or mutation during callus induction, callus redifferentiation and subculture proliferation in tissue culture, even if the seedlings are established, the traits of the parents are different. Therefore, Now it is necessary to design a rapid seedling propagation method of Actinidia jujube, which can realize the rapid and large-scale seedling propagation and cultivation of Actinidia jujube

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of propagation method of kiwi fruit fast growing seedling, comprises the following steps:

[0027] The first step, selection of explants: in November of each year, select 1-year-old robust plants, select branches with a stem thickness of 0.5 cm and full axillary buds, and cut 15 cm long cuttings, each with 3 buds , the upper cut of the insert is cut 1 cm away from the bud, and the cut is cut horizontally and sealed with paraffin, and the lower cut is cut obliquely at 45°;

[0028] The second step, pretreatment: put the insert obtained in the first step at a concentration of 0.05mg L -1 K 2 SO 4 Soak in the solution for 30min;

[0029] The third step, induction culture: remove the pretreated inserts in the second step, cover the inserts with a damp cloth with a thickness of 3mm, and place them in an environment with a temperature of 20°C and a humidity of 60%. Wash the cuttings with water at 25°C every morning and evening to obtain rooted and germinated cuttin...

Embodiment 2

[0033] A kind of propagation method of kiwi fruit fast growing seedling, comprises the following steps:

[0034] The first step, selection of explants: In November of each year, select 1-year-old robust plants, select branches with a stem thickness of 0.6cm and full axillary buds, and cut 16cm-long cuttings, each with 4 buds , the upper cut of the insert is cut 1 cm away from the bud, and the cut is cut horizontally and sealed with paraffin, and the lower cut is cut obliquely at 45°;

[0035] The second step, pretreatment: put the insert obtained in the first step at a concentration of 0.06mg L -1 K 2 SO 4 Soak in the solution for 32min;

[0036] The third step, induction culture: remove the pretreated inserts in the second step, cover the inserts with a damp cloth with a thickness of 4mm, and culture them in an environment with a temperature of 22°C and a humidity of 63%. Wash the cuttings with water at 25°C every morning and evening to obtain rooted and germinated cuttin...

Embodiment 3

[0040] Include the following steps:

[0041] The first step, explant selection: in November every year, select 2-year-old robust plants, select branches with a stem thickness of 0.6cm and full axillary buds, and cut 18cm-long cuttings, each with 4 buds , the upper cut of the insert is cut 2 cm away from the bud, and the cut is cut horizontally and sealed with paraffin, and the lower cut is cut obliquely at 45°;

[0042] The second step, pretreatment: put the insert obtained in the first step at a concentration of 0.08mg L -1 K 2 SO 4 Soak in the solution for 35min;

[0043] The third step, induction culture: remove the pretreated inserts in the second step, cover the inserts with a damp cloth with a thickness of 3mm, and place them in an environment with a temperature of 25°C and a humidity of 65%. Wash the cuttings with water at 25°C every morning and evening to obtain rooted and germinated cuttings;

[0044] The fourth step, seedbed preparation: choose dry soil with hig...

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Abstract

The invention discloses a propagation method for rapid seedling formation of actinidia arguta. The propagation method comprises the following steps of 1, explant selection, specifically, in November every year, selecting a robust plant growing for 1-2 years, selecting a branch with the stem thickness of 0.5-0.8 cm and full axillary buds on the plant, and cutting the branch into cutting sections with the length of 15-20 cm, and enabling each section to be provided with 3-5 buds; 2, pretreatment, specifically, putting the cutting sections obtained in the step 1 into a K2SO4 solution with the concentration of 0.05-0.13 mg.L<-1>, and soaking for 30-40 minutes; and 3, induction culture, specifically, fishing out the cutting sections pretreated in the step 2, covering the cutting sections with wet cloth with the thickness of 3-5 mm, culturing in an environment with the temperature of 20-32 DEG C and the humidity of 60%-75%, and washing the cutting sections with water with the temperature of 25 DEG C once every morning and evening in the period to obtain rooting and sprouting cutting sections. The method is low in cost, efficient, rapid, simple and high in operability, and can be widely applied to rapid large-scale seedling propagation cultivation of the actinidia arguta.

Description

technical field [0001] The invention relates to the technical field of kiwi fruit propagation, in particular to a method for rapidly growing seedlings of kiwi fruit. Background technique [0002] Jujube kiwifruit, also known as Chinese soft jujube kiwifruit, soft jujube, rattan melon, dog jujube, golden fruit tree, kiwi pear, kiwi fruit, soft jujube, deep mountain wood Polygonum, vine pear, small foreign peach, etc., is a vine deciduous plant of the Actinidiaceae Actinidia . The fruit is edible, has high nutritional value, contains a lot of vitamin C, starch, pectin, etc., and can be processed into jam, juice, preserved fruit, canned food, wine making or used to make cakes, candies and other foods. It can also be used medicinally, and has the functions of strengthening, antipyretic, invigorating the stomach, and stopping bleeding; the root and root bark have certain therapeutic and inhibitory effects on digestive tract cancer; the oil content of the seeds is 35.62%, which i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G7/06A01G9/28
CPCA01G2/10A01G7/06A01G9/28
Inventor 汤晓丽刘晓华郭晓秋雨杨婷婷李艳琪张芮
Owner LUDONG UNIVERSITY
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