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Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings

A tissue culture, hormone-free technology, applied in the agricultural field, can solve the problems of unsafe, uneven seedlings, high production costs, etc., and achieve the effect of easy operation, continuous production process, and fast seedling growth

Active Publication Date: 2015-05-13
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned problems existing in the prior art, the invention provides a method for hormone-free tissue culture and rapid propagation of clematis seedlings. Carry out frequent subculture, and do not use exogenous phytohormones in the whole cultivation process, and better solve the widespread use of exogenous phytohormones, high production costs, and uneven seedlings in the tissue culture process of A. One and the problem of unsafe food, not only greatly improved the quality of seedlings and the survival rate of transplanting, but also improved the safety of seedlings in use

Method used

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  • Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Wild clematis plants were collected from Nanjing County, Fujian Province and planted in a net room. After several months of observation, selected excellent plants were selected for artificial cross-pollination at the flowering stage. After 55 days, the fruit was selected to be plump, hard, free of pests and diseases, and the color of the appearance was red. or light brown capsules for tissue culture. Place the selected capsules on a sterile ultra-clean workbench, soak them in 75% alcohol for 50 seconds, take them out and transfer them to a 10% sodium hypochlorite solution for disinfection for 9 minutes, then take them out and transfer them to a sterile container for use. Soak and wash with sterile water for 4 times, and finally take out the capsule and dry the surface moisture with sterile paper, and then culture according to the following four tissue culture stages:

[0042] (1) Aseptic germination of seeds to form protocorms: After disinfecting and sterilizing the sur...

Embodiment 2

[0051] Select fine clematis plants planted in the clematis planting base, carry out artificial cross-pollination on the third day of full flowering, and select capsules with plump, hard, no pests and diseases, and reddish-brown appearance color for tissue culture after 60 days . Place the selected capsules on a sterile ultra-clean workbench, soak them in 75% alcohol for 30 seconds, take them out and transfer them to a 15% sodium hypochlorite solution for disinfection for 8 minutes, then take them out and transfer them to a sterile container for use. Rinse with sterile water for 5 times, and finally take out the capsule and dry the surface moisture with sterile paper, and then culture according to the following four tissue culture stages:

[0052] (1) Aseptic germination of seeds to form protocorms: After disinfecting and sterilizing the surface of the capsules, cut the capsules, take out the seeds and sow them evenly on the hormone-free seed germination medium, and place the s...

Embodiment 3

[0061]Collected from Yunnan wild clematis naturally pollinated, plump, hard, no pest damage and light brown in appearance. Place the selected capsules on a sterile ultra-clean workbench, soak them in 75% alcohol for 60 seconds, take them out and transfer them to an 8% sodium hypochlorite solution for disinfection for 10 minutes, then take them out and transfer them to a sterile container for use. Soak in sterile water for 3 times, and finally take out the capsule and dry the surface water with sterile paper, and then culture according to the following four tissue culture stages:

[0062] (1) Aseptic germination of seeds to form protocorms: After disinfecting and sterilizing the surface of the capsules, cut the capsules, take out the seeds and sow them evenly on the hormone-free seed germination medium, and place the sown culture bottles at a temperature of 20-28°C Cultivate and induce seed germination under light-free conditions. After 30 days of cultivation, the seeds swell a...

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Abstract

The invention relates to hormone-free tissue culture and a rapid propagation method of anoectochilus formosanus seedlings. The method comprises the following four tissue culture stages: 1) seeds germin ate without bacteria to form protocorms; 2) the protocorms is propagated and differentiation culture is performed on plantlets; 3) strong seedlings of aseptic seedlings are cultured; 4) rooting culture is performed, and different hormone-free culture media are adopted at different tissue culture stages. The method provided by the invention has the advantages that the operation is simple and convenient, the process is less, the cost is low, frequent subculture is not required to be performed on tissue culture seedlings, exogenous plant hormones are not used during the whole culture process, the problems that a large number of exogenous plant hormones are used, the production cost is high, the seedlings are irregular, and the seedlings are unsafe to eat exist in the conventional process of culturing anoectochilus formosanus tissues are solved better, the quality and the transplanting survival rate of the seedlings are improved, and security of using the seedlings is improved.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for hormone-free tissue culture and rapid propagation of clematis seedlings. Background technique [0002] Anoectochitus formosanus (Anoectochitus formosanus) is a rare and endangered Chinese herbal medicine. It belongs to the perennial herbaceous plant of Orchidaceae. It is recorded in the Pharmacopoeia of National Medicine, etc., and it is widely used among the people. The whole herb is used as medicine, and it is reported that it is used in the treatment of various common diseases and intractable diseases. Because of its wide range of treatments and unique curative effects, it is regarded as a "miracle medicine" by the people, and is called "the king of Chinese medicine" in Taiwan. It has been listed as one of the 29 rare plants that need to be protected in Taiwan, and Fujian Province is also listed as Provincial wild medicinal species protection. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈菁瑛
Owner 福建省农业科学院农业生物资源研究所
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