Technology for promoting forming and rooting of rhododendron fortunei callus

A callus and cuckoo technology is applied in the field of callus treatment for promoting callus formation of Rhododendron Yunjin, inducing rooting, and can solve the problems of difficult rooting, low survival rate, and small number of rooting of Rhododendron cuttings, so as to increase the formation rate. and callus area, good rooting effect and rot reduction effect

Inactive Publication Date: 2016-12-14
江西省·中国科学院庐山植物园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is the difficulty in rooting of rhododendron large-leaved evergreen cuttings, the small number of roots, and the low survival rate.

Method used

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  • Technology for promoting forming and rooting of rhododendron fortunei callus
  • Technology for promoting forming and rooting of rhododendron fortunei callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of technology that promotes Rhododendron yunnanensis callus formation and rooting, comprises the following steps:

[0018] 1. Seedbed laying: The seedbed is set in the glass greenhouse and adopts brick-concrete structure. Reserve one drainage hole every 80 cm at the bottom of the seedbed, and add stones, pebbles, and coarse sand to the bottom layer from bottom to top to increase the drainage and ventilation of the seedbed; install geothermal lines on the coarse sand to increase the ground temperature; then lay Peat + perlite + vermiculite = 3:1:1 volume ratio mixture of 8-10 cm thick, rake and lightly press to make the seedbed flat, water thoroughly; finally spray 500 times carbendazim aqueous solution to disinfect the substrate and surrounding environment .

[0019] 2. Collection of cuttings: Select 25-30-year-old, vigorously growing adult plants as the mother plant for ear picking, collect semi-lignified to lignified transitional branches of the year in late O...

Embodiment 2

[0025] Compared with Example 1, the only difference is that the cutting substrate described in "Step 1" is a mixture of peat+perlite=3:1 volume ratio.

[0026] This method was used to propagate Rhododendron versicolor through cuttings. After 6 months of cutting, it was found that the callus formation rate was 98.33%, the rooting rate was 67.50%, the number of adventitious roots was 23.19, the longest adventitious root length was 5.56 cm, and the root width was 4.26 cm.

Embodiment 3

[0028] Compared with the seedling raising effect of the previous research: the two-factor experiment using 6 substrates and 4 IBA concentrations in the early stage showed that among the 24 treatment combinations, "pure river sand substrate + 200 mg·L -1 The combination of soaking the base of cuttings (1.5-2.0 cm) for 16 h in the IBA aqueous solution had the best seedling raising effect, the callus formation rate was 96.67%, the rooting rate was 60.00%, the number of adventitious roots was 20.31, the longest adventitious root was 4.00 cm long, and the crown Width 3.18 cm. (See: "Forestry Science", 2015, 51 (9): 165-172)

[0029] It can be seen that, compared with the prior art, the method of the present invention can increase the rooting rate of Rhododendron yunnanensis cutting seedlings, especially significantly improve the quality of the root system.

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Abstract

The invention discloses technology for promoting forming and rooting of rhododendron fortunei callus. The technology includes: laying a seedbed; collecting a cutting; making a cutting; inducing rooting; performing cuttage and management. A geothermal line is adopted to heat the seedbed during rooting, an automatic intermittent spraying system moisturizes, air temperature is controlled at 15.0-20.0 DEG C, relative humidity is 85.0-95.0%, and a bactericide is sprayed every other 14d to reduce rotting of the cutting. By the technology, the problem that rhododendron fortunei is difficult in cuttage propagation rooting, low in rooting rate and small in number of roots are solved effectively, rooting rate is up to 73.67%, and root systems are strong; the technology is an effective propagation method for rhododendron fortunei and is of important significance in accelerating development of rhododendron industrialization in China.

Description

technical field [0001] The invention belongs to the technical field of plant cutting propagation, and in particular relates to a cutting callus treatment method and rooting induction technology for promoting the callus formation of rhododendron versicolor. Background technique [0002] Cutting propagation can not only maintain the excellent traits of the female parent, uniform seedling size, and early flowering and fruiting, but also has simple requirements for facility conditions, low cost, and easy promotion. It has been widely used in the cultivation of small-leaved deciduous and semi-evergreen Rhododendron (Azalea). Industrial seedlings. Restricted by genetic factors, the evergreen rhododendron subgenus ( Rhododendron Subgenus Hymenanthes) Rhododendron has difficulty in rooting and low seedling rate. In my country, seedlings are mainly raised by sowing. Although seed propagation has a large reproduction coefficient, the seedlings grow slowly, the flowering cycle is lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01C1/00
CPCA01G31/00A01C1/00A01G24/00
Inventor 张乐华李晓花王书胜单文王兆红魏宗贤刘向平
Owner 江西省·中国科学院庐山植物园
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