The Method of Creating New Germplasm by Remote Hybridization of Rhododendron

A distant hybridization and azalea technology, which is applied in the field of ornamental plant hybridization and breeding, can solve problems such as the cultivation of new varieties of incompatible azaleas, and achieve the effects of improving the hybrid fruit setting rate, avoiding secondary bagging and reducing workload.

Active Publication Date: 2021-05-04
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] The main purpose of the present invention is to provide a method for creating new germplasm by distant hybridization of rhododendrons in the field, so as to solve the problem of breeding new varieties of rhododendrons due to the incompatibility of distant hybridization between Rhododendron subgenus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Selection of parental parents: In order to obtain new varieties of Rhododendron with red flowers, fragrance and attractive leaves, the wild original Rhododendron yunnanensis was selected as the female parent. The main characteristics are: white flowers, fragrance, and pink young leaves. The male parent is the original species of azalea, and the pollen is collected from the park. The main characters are: red flower, large flower type, dense flowering, large pollen amount and high pollen vigor.

[0029] (2) Pollen collection and storage: collect the anthers of azalea at the early blooming stage and bring them back to the laboratory, place the anthers in a clean petri dish to dry naturally, and pick out the pollen with a planer after the anthers crack. The pollen was collected on a petri dish, dried on silica gel for 24 hours, sealed and stored in a -20°C refrigerator, and the pollen could be stored for 1 year.

[0030] (3) Manual emasculation and pollination of the f...

Embodiment 2

[0035] (1) Selection of parental parents: In order to obtain new varieties of Rhododendron with red flowers, fragrance and attractive leaves, the wild original Rhododendron yunnanensis was selected as the female parent. The main characteristics are: white flowers, fragrance, and pink young leaves. The male parent is the original species of Rhododendron lantana, and the pollen is collected from the park. The main characters are: red flower, large flower type, dense flowering, large pollen amount and high pollen vigor.

[0036] (2) Pollen collection and storage: collect the anthers of Rhododendron lantana at the early blooming stage and bring them back to the laboratory, place the anthers in a clean petri dish to dry naturally, and pick out the pollen with a planer after the anthers crack. The pollen was collected on a petri dish, dried on silica gel for 24 hours, sealed and stored in a -20°C refrigerator, and the pollen could be stored for 1 year.

[0037](3) Manual emasculati...

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Abstract

The invention belongs to the technical field of hybridization and breeding of ornamental plants, and specifically discloses a method for creating new germplasm by remote hybridization of rhododendrons in the wild. For healthy plants, the male parent is selected from artificially cultivated Rhododendron species, the pollen of the male parent is taken, and the female parent is emasculated and hybridized. Immediately after pollination, the stigma is wrapped with tinfoil prepared in advance. Make a good mark, the hybridization method used in the present invention can efficiently create new germplasm, and there are great differences in the flower color, flowering period, floral fragrance, leaf shape, leaf color, etc. of the parents used in the present invention, and their hybridization belongs to the subgenus Although the category of distant hybridization is relatively difficult, it is easy to generate new gene combinations and form innovative germplasm resources.

Description

technical field [0001] The invention belongs to the technical field of hybridization and breeding of ornamental plants, and in particular relates to a method for creating new germplasm by remote hybridization of rhododendrons in the wild. Background technique [0002] Rhododendron ( Rhododendron spp.) is Rhododendron (Ericaceae) Rhododendron ) is a general term for plants with high ornamental value. It is one of the ten traditional famous flowers in my country and also a famous flower in the world. China is the center of the origin and diversified distribution of Rhododendron. There are nearly 571 species of wild resources in the distribution, accounting for nearly 2 / 3 of the wild Rhododendron germplasm resources in the world. It is a huge Rhododendron germplasm resource bank. The introduction, cultivation and cross-breeding of rhododendrons started earlier in foreign countries. After nearly 300 years of continuous cross-breeding, tens of thousands of rhododendron garden ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 关文灵李叶芳马诗雨李凤荣
Owner YUNNAN AGRICULTURAL UNIVERSITY
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