The invention discloses a molding method of a rhesus monkey
pulmonary fibrosis model, a preparation, a preparation method of the preparation, the rhesus monkey
pulmonary fibrosis model and the application thereof. The molding method comprises the following steps: S1, performing
subcutaneous injection of
paraquat solution on rhesus monkey, and dripping
bleomycin solution to trachea of rhesus monkey; S2, generating the rhesus monkey
pulmonary fibrosis model. After molding is performed, the fact whether rhesus monkey molding is successful is diagnosed and the
efficacy of a
drug for treating pulmonary
fibrosis is evaluated through the observation of the clinical symptoms, autonomic activities, feed intake,
respiratory rate, haematological index, color dopplar
ultrasound and
lung tissue
pathological results of rhesus monkey. An
animal model built through the method, clinical symptoms, haematological indexes, color dopplar
ultrasound and
lung tissue
pathological alteration are similar to
lung fibrosis symptoms, moreover, compared with a model built by independently injecting
paraquat or independently dripping
bleomycin, a model of
paraquat and
bleomycin is higher in success rate and better in stability, and is applicable to the valuation of a new
drug for treating
lung fibrosis.