Rhesus monkey seizure model and drug screening method thereof

Abstract

The invention provides a method for preparing a rhesus monkey seizure model. The method comprises applying 0.5-0.7ml / kg of coriamyrtin to the rhesus monkey to induce the seizure. The invention also provides the application of the animal model in screening the drugs for treating the seizure. Compared with the other temporal epilepsy models, the research has the following advantages: (1) the drugs are systematically delivered by means of intramuscular injection, which is convenient to operate; (2) the seizure induction dosage is poorly correlated in the gender or age of the animal; (3) the repetition is good; (4) the electrode is convenient to install; (5) no direct mechanical damage is caused in the drain; (6) the seizure induction process is clear; and (7) the drug safety is good.

Description

Technical field [0001] The invention relates to an animal model of epileptic seizures in rhesus monkeys, in particular to the application of coriaria lactone in preparing epileptic seizure models of rhesus monkeys. Background technique [0002] Seizures (epileptic seizures or seizures, epileptic seizures) are epileptic brain dysfunction caused by abnormal discharge of brain neurons. Most of the seizures are short-lived and self-limited. Due to the different brain functional areas involved in the abnormal discharge, there may be a variety of clinical seizures, including partial or full seizures. The specific manifestations are motor, paresthesia, or behavioral cognition, Autonomic dysfunction. Generalized seizures and complex partial seizures are often accompanied by disturbances of consciousness of varying degrees. [0003] Epilepsy is the excessive synchronous abnormal discharge of neurons. Its mechanism is mainly the imbalance between the excitation and inhibition mechanisms in...

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Problems solved by technology

1997, 53: 239-258.) proposed that the drug-resistant epilepsy model should have the following characteristics: (1) the seizure type is similar to that of clinical drug-resistant epilepsy; (2) the model has paroxysmal abnormal discharges in the brain, And can evaluate drug efficacy from electrophysiological aspects; (3) AED cannot control its attack satisfactorily; (4) Model animals can survive for a long time

[0004] The only internationally recognized drug-resistant epilepsy model is the phenytoin-resistant amygdala electric ignition model in rats established by Loscher et al., but the disadvantages of this model are: complicated operation, difficult to raise animals, low ignition rate, easy death, and drug resistance. Narrow spectrum, mechanical damage to rat brain tissue

Guo L, Chai HX, Liu XL, Xie YG. Obserbations of synaptic efficacy and paired-facilitation in area CA1 of hippocampal slices from coriarialactone-kindled rats. Brain Res 1992; 572: 269-272. Wang Y, Zhou D, Wang B, LiHS, Chai HX, Zhou Q, Zhang SF, Stefan H.A kindling model of pharmacoresistant temporal lobe epilepsy in Sprague-dawley rats by corialaractone and its possible mechanism. Epilepsia 2003; 44: 475-488. Epilepsy is similar to the type of clinical temporal lobe epilepsy, and antiepileptic drugs cannot satisfactorily control its seizures. It has the advantages of simple establishment process, high ignition rate, and no mechanical damage. It is a better drug-resistant epilepsy model

[0007] Primates have the closest relationship with humans, and there are still no reports on the epilepsy model of rhesus monkeys at home and abroad, and there is no animal model of epileptic seizures constructed with ethanolactone and used for screening and treatment of epileptic seizures Drug-related reports

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