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A kind of preparation method of rhesus monkey diabetic nephropathy model

A technology of diabetic nephropathy and rhesus monkeys, which is applied in the field of preparation of rhesus monkey diabetic nephropathy models, can solve the problems of poor drug treatment effect and achieve the effect of strong repeatability and simple administration method

Active Publication Date: 2018-09-18
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once DN patients have significant clinical symptoms, there are fewer drugs to choose at this time, and the therapeutic effect of drugs is poor, and the disease often develops progressively until end-stage renal failure, which brings a huge economic burden to the patient's family and society.

Method used

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  • A kind of preparation method of rhesus monkey diabetic nephropathy model
  • A kind of preparation method of rhesus monkey diabetic nephropathy model
  • A kind of preparation method of rhesus monkey diabetic nephropathy model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Preparation of rhesus monkey diabetic nephropathy model of the present invention

[0035] 1 Experimental materials and instruments

[0036] 1.1 Basic information of the test product

[0037] IL-1β antibody: abcam

[0038] TNF-a antibody: abcam

[0039] Antibody to TGF-β1: Bioworld

[0040] CTGF antibody: abcam

[0041] Collagen IV antibody: abcam

[0042] Fibronection Antibody:Bioworld

[0043] Antibody to MCP-1: ebioscience

[0044] CD68(KP1) antibody: abcam

[0045] eNOS antibody: abcam

[0046] Animal Tissue Total RNA Extraction Kit: Tiangen

[0047] iScript TM cDNA synthesis kit: BIO-RAD

[0048] iQ TM Green Supermix: BIO-RAD

[0049] 1.2 The main instruments and equipment required for the test

[0050] Blood glucose detector: Luokang full vitality blood glucose detector, Luokang full vitality blood glucose test strip

[0051] Automatic blood biochemical analyzer (Roche COBAS Integra400Plus, Switzerland, number MEB-02-01)

[0052] BIO-...

Embodiment 2

[0136] Embodiment 2 screens the medicine for the treatment of diabetic nephropathy with the model of the present invention

[0137] a, the rhesus monkey diabetic nephropathy model established according to the method of Example 1;

[0138] b. administering the drug candidate to an animal model;

[0139] c. Observe the effect of candidate drugs on various indicators of metabolic syndrome, and evaluate potential drugs for treating diabetic nephropathy.

[0140] In summary, the modeling method of the present invention induces clinical symptoms of diabetic nephropathy in rhesus monkeys through long-term blood sugar control. In addition, the combination of long-term blood sugar control and high fat intake can also induce clinical symptoms of diabetic nephropathy in rhesus monkeys. symptoms, and the symptoms are more significant, indicating that the two methods of the present invention can effectively establish a rhesus monkey diabetic nephropathy model.

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PUM

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Abstract

Disclosed is a method for preparation of a rhesus monkey diabetic nephropathy (DN) model, comprising the following steps: (1) administering streptozocin with the dosage of 80-100 mg / Kg to a rhesus monkey; (2) when the blood glucose concentration is increased to 11.1 mmol / l, administering insulin before a meal, such that the blood glucose concentration of the rhesus monkey is 10-20 mmol / L. The present invention employs a blood glucose control method on a rhesus monkey having diabetes mellitus (DM), and successfully induces a rhesus monkey DN model.

Description

technical field [0001] The invention relates to a preparation method of a rhesus monkey diabetic nephropathy model. Background technique [0002] The incidence of Diabetes Mellitus (DM) is increasing rapidly. According to reports, there are about 92 million DM and 148 million pre-DM patients in China, becoming the second country with the largest incidence of diabetes after the United States. With the gradual development of the disease, about 30-40% of these patients with type 1 diabetes and about 20% of type 2 diabetes patients are accompanied by severe renal damage-diabetic nephropathy (Diabetic Nephropathy, DN). [0003] DN is one of the most serious chronic complications of diabetic patients and the leading cause of death in diabetic patients. Once DN patients have significant clinical symptoms, there are fewer drugs to choose, and the therapeutic effect of drugs is poor. The disease often develops progressively until end-stage renal failure, which brings a huge economi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/28A61K31/7008A61K49/00A01K67/02A23K20/158A23K20/163A23K20/10A23K50/00
CPCA61K49/0008A01K67/02A01K67/027A01K2207/25A01K2227/106A01K2267/0362A23K50/00A61K38/28
Inventor 陆燕蓉程惊秋陈又南刘敬平刘芳杨立川王莉李宏霞张杰王丹何斯荣王成世史梅梅袁玉佳赵久茗
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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