The invention relates to a
microorganism as a carrier of
nucleotide sequences coding for antigens and
protein toxins comprising the following components: (I) at least one
nucleotide sequence coding for at least one complete or partial
antigen of at least one wild-type or
mutated protein; and (II) at least one
nucleotide sequence coding for at least one
protein toxin and / or at least one
protein toxin subunit; and (III) a) at least one nucleotide sequence coding for at least one
transport system which enables the expression of the expression products of component (I) and component (II) on the outer surface of the
microorganism and / or enables the
secretion of the expression products of component (I) and component (II); and / or coding for at least one
signal sequence which enables the
secretion of the expression products of component (I) and component (II); and / or (III) b) optionally, at least one nucleotide sequence coding for at least one protein for lysing the
microorganism in the
cytosol of mammalian cells and for intracellularly releasing plasmids or expression vectors, which are contained in the lysed microorganism; and (IV) at least one nucleotide sequence for at least one activation sequence for the expression of one or more of components (I) to (III), wherein said activation sequence can be activated in the microorganism and / or is
tissue cell-specific, tumor
cell-specific, macrophage-specific,
dendrite-specific,
lymphocyte-specific, function-specific or non-
cell-specific”; wherein any of components (I) to (IV) can be present either once or several times and either identical or different. Also disclosed are a process of manufacturing thereof, corresponding plasmids or expression vectors and uses of the microorganism as a medicament.