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Cyfluthrin hapten compound, its synthesis method and use

A technology for fenvalerate and a synthesis method, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve problems such as affecting food safety, environmental threats, etc., and achieves a simple synthesis method, good affinity and high purity. Effect

Inactive Publication Date: 2006-06-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, fenvalerate is the most widely used variety of pyrethroid pesticides in my country, whether it is the amount per unit area or the total amount. The excess of fenvalerate residues in agricultural products directly affects food safety and poses a certain threat to the environment.

Method used

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  • Cyfluthrin hapten compound, its synthesis method and use
  • Cyfluthrin hapten compound, its synthesis method and use
  • Cyfluthrin hapten compound, its synthesis method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of fenvalerate artificial hapten, molecular structural formula is (n=2 at this moment):

[0037]

[0038] It is used as the raw material of the antigen system for animal immunization.

[0039] The synthetic method of above-mentioned fenvalerate artificial hapten is as follows:

[0040] 1) Synthesis of 2-cyano-3-phenoxybenzyl alcohol: Add 7.20g (0.14mol) sodium cyanide and 20ml water into a 250ml two-necked flask, stir and dissolve, then add 40ml toluene, 20.60g (0.1mol) m-phenoxybenzaldehyde (2), 0.65g tetrabutylammonium bromide, add dropwise 15ml36~38% hydrochloric acid at room temperature, continue to react for 1.5hr after adding, then add 12.5ml water, dissolve the solid wherein, use The aqueous layer was separated by a separatory funnel to obtain a pale yellow organic phase (mainly containing compound (3)).

[0041] Put the light yellow organic phase into a 250ml three-neck flask, add 23.0ml (0.18mol) of 36-38% hydrochloric acid, stir overnight at room t...

Embodiment 2

[0052] A kind of fenvalerate artificial hapten, molecular structural formula is (n=5 at this moment):

[0053]

[0054] It is used as the raw material of the antigen system for animal immunization.

[0055] The synthetic method of above-mentioned fenvalerate artificial hapten is as follows:

[0056] 1) Synthesis of 2-cyano-3-phenoxybenzyl alcohol Add 7.20g (0.14mol) sodium cyanide and 20ml water into a 250ml two-necked flask, stir to dissolve, add 40ml toluene, 20.60g (0.1mol) methane Phenoxybenzaldehyde (2), 0.65g of tetrabutylammonium bromide, cooled to below 0 degrees with an ice-water bath, dropwise added 15ml of 36-38% hydrochloric acid, controlled the speed, finished dropping in about 30 minutes, and continued to react for 1.5 minutes after adding hr, and then add 12.5ml of water to dissolve the solid therein, and separate the water layer with a separatory funnel to obtain a light yellow organic phase (mainly containing compound (3)).

[0057] Put the light yellow o...

Embodiment 3

[0068] Preparation of the immunogen:

[0069] The immunogen was synthesized using the carbodiimide method. Dissolve compound (1) (QW-PS) or (QW-He) (50-80 micromoles) in 1-2 mL of N,N-dimethylformamide, and then add an equivalent amount of Dicyclohexylcarbodiimide and N-hydroxysuccinimide, let it react overnight at room temperature, centrifuge, take 500-800 μL of the supernatant and add it to 4-8 mL of 15-20 mg / mL bovine serum albumin carbonate In the buffer solution, it should be added slowly, and then react with magnetic stirring for 4 to 6 hours. After the reaction is completed, put it into a dialysis bag, first dialyze with distilled water for 2 to 4 times, and then use 0.8 to 0.9% normal saline After dialysis, aliquots were stored in a refrigerator at -20°C.

[0070] Identification of artificial antigens:

[0071] According to the proportion of hapten, carrier protein and coupling product used in the immune antigen reaction of synthetic fenvalerate, carry out ultraviol...

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Abstract

The invention discloses a fenvalerate artificial partial antigen, the molecular structure is in the right, and n=1-5. The invention also provides the method for synthesizing fenvalerate artificial partial antigen, and the application of the product as raw material of antigen system for animal immunization.

Description

technical field [0001] The present invention relates to selecting a compound having -COOH and possibly containing the original structure of fenvalerate as the hapten of fenvalerate and the synthesis method and application of the hapten. Background technique [0002] The invention belongs to the technical field of immunochemistry and residue analysis of small-molecule pesticide compounds (molecular weight less than 1000 Daltons), relates to organic synthesis, immunochemistry and biochemistry, and relies on basic principles of immunology and immunochemistry and biotechnological means to design and synthesize Small molecule target analyte hapten, and coupled with carrier protein to prepare effective artificial antigen, immunize animals to prepare specific antibody for small molecule analyte, use the specific immunological reaction of antigen and antibody and the identification of markers that are easily detected and identified Amplification, quantitative detection of ultra-trac...

Claims

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Application Information

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IPC IPC(8): C07C235/34C07C231/10
Inventor 程敬丽朱国念朱烈桂文君金仁耀金茂俊
Owner ZHEJIANG UNIV
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