37 results about "Ne carboxymethyl lysine" patented technology

A method for determining the levels of biomarkers, specifically, advanced glycation end products (AGEs) and oxidation products (Ops) in a biological sample such as a plasma ultrafiltrate, is used to determine a patient's risk and / or rate of developing diabetes related nephropathy. The preferred biomarkers to measure include Nε-(1-carboxyethyl-lysine (CEL), methylglyoxyl-derived hydroimidazolone (MGHI) and Nε-carboxymethyllysine (CML). Also provided herein is a method of diabetic care which includes determining a diabetic patient's risk of developing diabetes related kidney disease and adjusting the patient's treatment regimen to include in addition to glucose lowering agents, additional treatments such as medications that modify the renin-angiotensin system, or specialized diets with low levels of AGEs or oxidative products.

The invention relates to the technical field of analysis and detection, and provides a method for synchronously detecting carboxymethyl lysine and carboxyethyl lysine in green tea in order to solve the problem that an existing method for detecting CML and CEL will be affected by different raw material substrates. d4-CML and d4-CEL are used as interior labels, a multi-choice reaction monitoring mode is adopted, a quantitative analysis method for synchronously detecting CML and CEL in green tea is built through methodological argumentation, and a foundation is provided for monitoring and controlling AGEs in tea. The detection method is good in accuracy, reproducibility and stability, and applicable to practical detection of CML and CEL in green tea.

The invention discloses a detecting method of carboxymethyl lysine (CML). The method is characterized by measuring the content of the CML in food adopting high performance liquid chromatography-tandem mass spectrometry in combination with an HILIC (Hydrophilic Interaction Liquid Chromatography) column for strong-polarity CML separation under a higher organic phase proportion. Crushed food passes through an MCX (Mixed-Mode Cation-Exchange) small column for being extracted and purified after being reduced by sodium borohydride and hydrolyzed by hydrochloric acid; sample liquor is subjected to gradient elution in the HILIC column by taking methanol-water (containing 2 mM ammonium acetate and 0.1% formic acid) as a mobile phase, so that CML is separated from a sample substrate; tandem mass spectrum analysis is carried out by using an electrospray ion source, positive ion scanning and a multi-reaction monitoring mode; quantitative analysis is carried out by using D4-CML as an internal standard substance, wherein analysis time is 12 minutes, and CML has an absorption peak at the retention time of 3.47 minutes. The detecting method disclosed by the invention is high in sensitivity, high in accuracy and good in precision, does not need to carry out derivatization onto samples, simplifies sample pre-treatment, shortens detecting time and saves detecting cost.

The invention provides a separating purification method of organically synthesized carboxy methyl lysine. Separating purification is a key step for finally obtaining high-purity carboxy methyl lysine; preparative liquid chromatography and ion exchange chromatography separating purification methods are used at present in the prior art and these methods are relatively cumbersome, low in efficiency and not suitable for mass preparation. To explore quick, efficient and low-price separating purification method becomes very important for obtaining a large quantity of low-cost high-purity carboxy methyl lysine monomers. The separating purification method of carboxy methyl lysine is innovated boldly in the invention, the synthesis amount of carboxy methyl lysine is improved to the gram grade, the purification efficiency of carboxy methyl lysine is increased greatly, the experiment cost is lowered, and a foundation is further laid for carrying out various toxicological evaluations on carboxy methyl lysine.

The invention relates to a method for researching an inhibiting effect of PQQ (pyrroloquinoline quinone) on AGEs. The method is a researching result of the inhibiting effect of PQQ and later period glycosylation end product AGEs after the method for searching on reaction of PQQ, Lys and Arg and belongs to the technical field of bioanalysis. N epsilon-(carboxymethyl)lysine (CML) and N epsilon-(carboxyethyl)lysine (CEL) are two of the most primary monomers of AGEs. The invention optimizes and improves the method for determining CML and CEL, adopts n-butanol / HCl derivative reaction, solid-phase extraction and UPLC-MS / MS for detecting the contents of CML and CEL in plasma and has the characteristics of high efficiency, accuracy and sensitivity; the established method is adopted for analyzing the change of CML, CEL, protein-binding CML and protein-binding CEL after a D-gal (D-galactose) induced aging rat is intervened with a certain amount of donepezil (Don) and PQQ, so as to supply a new basis for pathogenesis and drug targets of degenerative diseases.

The invention provides a distiller's grain extract, a preparation method and application thereof. The extraction method is as follows: in a protective atmosphere, CdSe / ZnS quantum dots modified by trioctylphosphine, glyoxal dimethacrylate, 4-vinylpyridine and gallic acid were induced by azobisisobutyronitrile in two React in toluene, purify and dry to obtain the filler for extracting the antioxidant components of distiller's grains, and prepare it into a solid phase extraction column; add the distiller's grains to the ethanol-water mixture, heat and stir, and collect the extract; use the self-prepared solid phase extraction column Distiller's grain components are extracted, and distiller's grain extract is obtained after drying. The distiller's grain extract obtained by this method contains phenolic acids and can be used as a food additive to inhibit the production of carboxymethyllysine during food processing, expanding the new application of distiller's grains in liquor and providing a new source for resource utilization of distiller's grains way. The method for preparing distiller's grains extract provided by the invention has the advantages of simple and convenient extraction process, low cost, easy industrial application, and large amount of distiller's grains can be processed.

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.

The invention provides an all-natural edible spice package and a method for inhibiting the production of pyrogen-bound carboxymethyllysine in traditional stewed meat products. The spice package includes the following components by weight: 2.25-5.25 parts of star anise, 1.5-3.5 parts of fennel, 0.75-1.75 parts of cinnamon, 0.75-1.75 parts of bay leaves, 0.75-1.75 parts of Angelica dahurica, and 0.75-1.75 parts of Kaempferia , 0.38-0.88 parts of tangerine peel, 0.38-0.88 parts of grass fruit, 0.15-0.35 parts of cardamom. The invention achieves the purpose of significantly reducing the generation of pyrogen-bound carboxymethyl lysine by adding compound natural edible spices rich in natural antioxidants such as phenols and flavonoids in the stewed pork process. The invention is safe and reliable, has simple process steps, low cost and strong operability, and has great market potential and application promotion value.

The invention discloses a silicon dioxide particle composite material coated with polymeric ionic liquid, a preparation method of the composite material and application of the composite material in removal of carboxy methyl lysine in fermented soy sauce, and belongs to the technical field of food safety and quality. The silicon dioxide particle composite material coated with the polymeric ionic liquid is prepared from silicon dioxide particles, polyethylene nonylphenol ether and propyl alcohol through an emulsion polymerization method. The preparation method comprises the specific steps that the silicon dioxide particles, polyethylene nonylphenol ether, functionalized ionic liquid containing vinyl and propyl alcohol are mixed and ultrasonically dispersed; the materials are heated to 70 DEG C to 76 DEG C under nitrogen protection, and reacting under stirring is conducted for 24 hours to 48 hours; the materials are cooled to room temperature, centrifugal separation is conducted, the collected solid phase is the silicon dioxide particle composite material coated with the polymeric ionic liquid, and the composite material is used for removing carboxy methyl lysine in the fermented soy sauce. The method is easy and convenient to operate, low in cost, simple in treatment technology and high in removal efficiency.

The present invention discloses a method for inhibiting the production of carboxymethyllysine during the thermal processing of minced fish products. The method includes the following specific steps: during the chopping and mixing of the minced fish, carboxymethyllysine inhibitors with mass of 0.3-0.8% of the mass of the minced fish are added; the carboxymethyllysine inhibitors are prepared from glutamine transaminage and chitosan oligosaccharides with a mass ratio of 1 to 1-1.1 through mixing. Carboxymethyllysine inhibitors which consist of transglutaminase and chitosan oligosaccharides are added during the chopping and mixing of the minced fish to achieve the purposes of inhibiting the production of carboxymethyllysine during the thermal processing of the minced fish products. The processing steps are simple, are strong in operability, and have great popularization and application values.

The invention relates to a detection method and application of a carboxy methyl lysine ingredient in food. The detection method comprises the following steps of: carrying out derivatization by a derivating agent after protein acid in a sample is hydrolyzed; obtaining solution to be detected from the derived solution by solid-phase extraction; carrying out qualitative and quantitative analysis on the carboxy methyl lysine ingredient in the food by adopting a tandem mass spectrometry of isotope dilution ultra-performance liquid chromatography, wherein the derivating agent is 9-fluorenylmethyl chloroformate.

The invention relates to a method for detecting carboxy methyl lysine (CML) in soybean sauce. The method comprises the following steps: firstly, carrying out detection pretreatment on CML in a soybean sauce system, namely, detection pretreatment on free-state CML and combined-state CML; carrying out detection pretreatment on the free-state CML, namely sampling, adding a methanol solution to dilute, swirling and evenly mixing, purifying through a C18 solid-phase extraction column, adding a methanol solution to elute, filtering through a micro-filtration membrane to obtain a sample 1 to be detected; carrying out the detection pretreatment on the combined-state CML, namely sampling, reducing through sodium borohydride, separating polypeptide and protein through a dialysis bag, hydrolyzing through hydrochloric acid, disacidifying through vacuum, purifying through the C18 solid-phase extraction column, filtering through the micro-filtration membrane, drying in vacuum, re-dissolving to obtain a sample 2 to be detected; and subsequently, carrying out CML detection on the sample 1 and the sample 2 to be detected respectively. A total content of the CML is sum of the content of the free-state CML and the content of the combined-state CML. The sample pretreatment method has the characteristics of elimination of interference, protection of chromatographic column, safety and reliability, and capability of completely analyzing all the CML in the soybean sauce.

The invention discloses a method for measuring the interaction efficiency of quinone substances and carboxymethyl lysine (CML) in solution. The method measures the cyclic voltammetry curves of the catechols solution before and after the addition of CML, records the peak current and voltage characteristic values ​​of the catechols solution before and after the addition of CML, and then calculates the reduction rate of the reduction peak current by calculating , the interaction efficiency between quinones in solution and CML can be obtained. In the method of the invention, after the phenolic substances are oxidized to form quinones, they can quickly interact with the CML in the system to reduce the side reactions of the quinones; the amount of substances attached to the electrode surface is small, and the detection accuracy is high; In the prior art, the adhesion of polyphenols and their oxidation products to the working electrode, the related side reactions of quinones and other factors interfere with the interaction between quinones and CML; at the same time, the assay method of the invention is simple, the analysis time is short, and the reproducibility is good. and convenience, low cost of use, and wide application.