A distiller's grain extract and its preparation method and application

A technology for extract and distiller's grains, which is applied in the field of preparation of liquor distiller's grains extract, can solve the problems of low utilization rate of distiller's grains extract and the like, achieves prevention of harm to human body, easy industrial application, good inhibition of carboxymethyl lysine production the effect of the ability

Active Publication Date: 2022-02-25
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low utilization rate of distiller's grains extract, the present invention provides a distiller's grains extract and a preparation method thereof, the extraction process is simple, the cost is low, and it is easy for industrial application, and a large amount of distiller's grains can be processed

Method used

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  • A distiller's grain extract and its preparation method and application
  • A distiller's grain extract and its preparation method and application
  • A distiller's grain extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of filler for extracting distiller's grain antioxidant components

[0039] 1. Experimental materials and instruments

[0040] Gallic acid: Shanghai Tian Biotechnology Co., Ltd.; 4-vinylpyridine, ethylene glycol dimethacrylate, azobisisobutyronitrile: Shanghai McLean Biochemical Technology Co., Ltd.; trioctylphosphine modified CdSe / ZnS quantum Dot: Wuhan Jiayuan Quantum Dot Technology Development Co., Ltd.; Methanol: Sinopharm Chemical Reagent Co., Ltd.; Acetone: Sinopharm Chemical Reagent Co., Ltd.; Acetonitrile: Sinopharm Chemical Reagent Co., Ltd.; Xylene: Sinopharm Chemical Reagent Co., Ltd.; Distiller's grains: sorghum as the main raw material, the residue after solid-state fermentation, obtained after freeze-drying; casein and D-glucose: Xilong Technology Co., Ltd.

[0041] Agilent 1260 infinity liquid chromatography-mass spectrometry (Agilent, USA).

[0042] 2. Optimization of the preparation conditions of the filler

[0043] Taking the in...

Embodiment 2

[0057] Example 2 Preparation of distiller's grain extract

[0058] 1. Experimental materials and instruments

[0059] Distiller's grains: sorghum as the main raw material, the residue after solid-state fermentation, obtained after freeze-drying; casein and D-glucose: Xilong Technology Co., Ltd.; water (DDW): prepared using WaterPro water purification system (Labconco Corp.).

[0060] Agilent 1260 infinity liquid chromatography-mass spectrometry (Agilent, USA).

[0061] 2. Optimization of extraction conditions

[0062] Taking the inhibition rate of carboxymethyl lysine as an index, a single factor experiment method was adopted,

[0063] Weigh 0.2 g of distiller's grains and add 10 mL of ethanol-water mixture (volume ratios of ethanol:water are 6:4, 7:3, 8:2, 9:1, 100% ethanol) as solvent, heat and stir at 60°C After extraction for 30 min, after solid-liquid separation, the extract was collected; 1.5 mL distiller’s grains extract was loaded onto a solid-phase extraction colum...

Embodiment 3

[0090] Example 3 Antioxidant properties of distiller's grains extract and inhibition rate to carboxymethyllysine

[0091] 1. Experimental materials and instruments

[0092] DPPH: Yuanye Biotechnology Co., Ltd.; FRAP kit: Beijing Banxia Technology Co., Ltd.; casein and D-glucose: Xilong Technology Co., Ltd.; disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate and aminoguanidine: J&K Science Ltd.; Agilent 1260 Infinity LC-MS (Agilent, USA).

[0093] 2. Experimental procedure

[0094] (1) Dissolve distiller's grain extracts C1, C2, and S1 in 5 mL of 70% acetone-water solvent respectively, and set aside.

[0095] (2) Scavenging ability for DPPH: Take 150 μL distiller’s grain extract and add to 2.85mL 6.6×10 -5 After mixing in mol / L DPPH solvent and reacting in the dark for 2 hours, measure the absorbance at 515nm. The inhibition rate of DPPH is:

[0096] Inhibition rate (%) = (1-A s / A c )×100%

[0097] Where As is the absorbance of the sample and Ac is th...

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Abstract

The invention provides a distiller's grain extract, a preparation method and application thereof. The extraction method is as follows: in a protective atmosphere, CdSe / ZnS quantum dots modified by trioctylphosphine, glyoxal dimethacrylate, 4-vinylpyridine and gallic acid were induced by azobisisobutyronitrile in two React in toluene, purify and dry to obtain the filler for extracting the antioxidant components of distiller's grains, and prepare it into a solid phase extraction column; add the distiller's grains to the ethanol-water mixture, heat and stir, and collect the extract; use the self-prepared solid phase extraction column Distiller's grain components are extracted, and distiller's grain extract is obtained after drying. The distiller's grain extract obtained by this method contains phenolic acids and can be used as a food additive to inhibit the production of carboxymethyllysine during food processing, expanding the new application of distiller's grains in liquor and providing a new source for resource utilization of distiller's grains way. The method for preparing distiller's grains extract provided by the invention has the advantages of simple and convenient extraction process, low cost, easy industrial application, and large amount of distiller's grains can be processed.

Description

technical field [0001] The invention belongs to the field of resource utilization of waste, and in particular relates to a preparation method of liquor lees extract. Background technique [0002] Advanced glycation end products (Advanced Glycation End Products, AGEs) are formed in the second reaction stage of MR, that is, the late stage, which is caused by the reaction of protein with aldose and non-enzymatic glycosylation or the reaction of lipid with aldose. A class of compounds formed by oxidation. Carboxymethyllysine (carboxymethyllysine) has no fluorescent properties, no cross-linking properties, and is acid-stable. Carboxymethyllysine is the most studied and most representative advanced glycation terminal The product, carboxymethyllysine, is often used as the primary target assay for the quantification of AGEs in food. [0003] Natural polyphenols have various biological activities such as anti-oxidation and anti-apoptosis, and have a great effect on promoting gastro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/10
Inventor 刘慧琳王静王玉珍
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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