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Method for detecting advanced glycation end products (AGEs) in main stream smoke of cigarette

A technology for mainstream smoke and end products of cigarettes, applied to measuring devices, instruments, scientific instruments, etc., can solve tedious and time-consuming problems, achieve the effects of eliminating false positives, simple and efficient pretreatment, and high sensitivity

Active Publication Date: 2015-05-20
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

Due to the particularity of the matrix of food samples, it is generally necessary to perform degreasing, reduction, protein precipitation, protein hydrolysis and other steps on the sample first. [3] , followed by solid-phase extraction technique [3] or derivatization reaction [4] In order to improve the detection sensitivity, the pre-treatment is very tedious and time-consuming

Method used

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  • Method for detecting advanced glycation end products (AGEs) in main stream smoke of cigarette
  • Method for detecting advanced glycation end products (AGEs) in main stream smoke of cigarette
  • Method for detecting advanced glycation end products (AGEs) in main stream smoke of cigarette

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The balanced and sieved cigarettes are smoked in accordance with the standard conditions specified in YC / T29-1996, and the total particulate matter of 4 cigarettes is collected by using Cambridge filters. Place the filter in a 50 mL conical flask with a stopper, add 100 μL of d4-CML and d4-CEL solutions, add 30 mL of 0.1M hydrochloric acid solution, and ultrasonically extract for 30 minutes. Take 15mL of extraction solution and load it on Dikma ProElut PXC ( 150mg / 6mL / 30pkg) cation exchange solid-phase extraction column (3mL methanol and 3mL 0.1M hydrochloric acid are used for activation before loading), followed by 3mL 0.1M hydrochloric acid and 3mL water rinse, and finally 10mL 5% ammonia-methanol solution Take off. In the process of solid phase extraction, the liquid flows down naturally by gravity. The eluent adopts nitrogen blowing concentrator (water bath temperature is 35 o C) Blow-dry, then add 1mL of water to reconstitute, the solution is added to the chrom...

Embodiment 2

[0034] Select the Kentucky reference cigarette 3R4F and 10 samples of commercial cigarettes, respectively smoke according to the standard conditions specified in YC / T 29-1996, and use Cambridge filters to smoke the total particulate matter of 4 cigarettes, and then follow the instructions in Example 1. Steps for sample processing and analysis. The result of the experiment was that neither CML nor CEL was detected in the mainstream smoke of the Kentucky reference cigarette 3R4F and 10 commercial cigarette samples.

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Abstract

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.

Description

technical field [0001] The invention belongs to the analysis and detection technology research of harmful substances in cigarette mainstream smoke, and specifically relates to a liquid chromatography-tandem mass spectrometry method for detecting advanced glycation end products (AGEs) (including carboxymethyllysine) in cigarette mainstream smoke (CML) and carboxyethyllysine (CEL)) methods. Background technique [0002] Advanced glycation end products (AGEs) are produced by the reaction of sugar and protein or fat, and mainly exist in foods cooked at high temperatures. When the content of AGEs is high, the human immune system is in an inflamed state, which has harmful effects such as promoting inflammation, cardiovascular disease, diabetes and accelerated aging [1] . The most studied AGEs are carboxymethyllysine (CML) and carboxyethyllysine (CEL). The formation of AGEs is related to the Maillard reaction. It is formed by the reaction of reducing sugars, proteins, and fats....

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 余晶晶颜权平王昇赵阁王冰蔡君兰丁丽于永杰谢复炜张晓兵
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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