A method for measuring the freshness of royal jelly

A royal jelly and freshness technology, applied in biological testing, material inspection products, fluorescence/phosphorescence, etc., can solve the problems of regional differences and expensive detection instruments, and achieve the effect of simple preparation and reliable detection methods

Inactive Publication Date: 2017-10-17
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these determination methods all have defects such as regional differences and expensive testing instruments.
So far, there is no internationally recognized indicator that can truly reflect the freshness of royal jelly and can be promoted in practical applications

Method used

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Experimental program
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Embodiment Construction

[0008] A method for measuring the freshness of royal jelly, the method measures the content changes of Pentodilysine and CML (carboxymethyl lysine) in royal jelly by three-dimensional fluorescence spectrometry and ELISA (enzyme-linked immunosorbent) method respectively.

[0009] The three-dimensional fluorescence spectroscopy method refers to a method of wavelength scanning.

[0010] The ELISA method uses an ELISA kit.

[0011] The Pentodilysine and CML (carboxymethyl lysine) are the products of Maillard reaction during the storage of royal jelly □ glycosylation terminal products.

[0012] The extraction method of the Pentodilysine is that the ratio of royal jelly:water:trichloroacetic acid is 1:5:5, 12000rpm / min, 10min, and the supernatant is taken.

[0013] The extraction method of the CML is that the royal jelly is dissolved in water at a ratio of 1:5.

[0014] The parameters of the wavelength scanning are: λex: 320nm, λem: 380□500nm, scanning speed: 240nm / min, delay time...

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Abstract

A new method for measuring the freshness of royal jelly is disclosed. The method mainly monitors the freshness of the royal jelly by measuring the glycosylation terminal products Pentodilysin and CML (carboxymethyllysine) in the royal jelly by three-dimensional fluorescence spectroscopy and ELISA (enzyme-linked immunosorbent) method respectively. So far, there is no internationally recognized index that can truly reflect the freshness of royal jelly and can be promoted in practical applications.

Description

technical field [0001] The invention relates to the detection of the freshness of royal jelly. Background technique [0002] Fresh royal jelly is rich in biologically active substances, which have a high nutritional and health effect on the human body, and have achieved significant curative effects in clinical applications, such as regulating immunity, anti-fatigue, anti-aging, anti-tumor, and improving sleep. It has a good auxiliary therapeutic effect on the digestive system, circulatory system, cardiovascular and cerebrovascular systems, etc., and is called "friend of human health". [0003] The composition of royal jelly is extremely complex and contains a variety of active substances. These active ingredients are extremely sensitive to temperature. If the storage method is not appropriate, its physical properties, chemical composition, physiological functions and other qualities will change significantly. Therefore, the key to the quality of royal jelly lies in its fres...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/68
Inventor 张红城高铁俊刘娟
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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