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Vinasse extract and preparation method and application thereof

A technology for extract and distiller's grains, which is applied in the field of preparation of liquor lees extract, can solve problems such as low utilization rate of distiller's grains extract, and achieve the effects of preventing harm to human body, expanding new applications and facilitating industrial application.

Active Publication Date: 2019-05-28
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low utilization rate of distiller's grains extract, the present invention provides a distiller's grains extract and a preparation method thereof, the extraction process is simple, the cost is low, and it is easy for industrial application, and a large amount of distiller's grains can be processed

Method used

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  • Vinasse extract and preparation method and application thereof
  • Vinasse extract and preparation method and application thereof
  • Vinasse extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of filler for extracting distiller's grain antioxidant components

[0039] 1. Experimental materials and instruments

[0040] Gallic acid: Shanghai Tian Biotechnology Co., Ltd.; 4-vinylpyridine, ethylene glycol dimethacrylate, azobisisobutyronitrile: Shanghai McLean Biochemical Technology Co., Ltd.; trioctylphosphine modified CdSe / ZnS quantum Dot: Wuhan Jiayuan Quantum Dot Technology Development Co., Ltd.; Methanol: Sinopharm Chemical Reagent Co., Ltd.; Acetone: Sinopharm Chemical Reagent Co., Ltd.; Acetonitrile: Sinopharm Chemical Reagent Co., Ltd.; Xylene: Sinopharm Chemical Reagent Co., Ltd.; Distiller's grains: sorghum as the main raw material, the residue after solid-state fermentation, obtained after freeze-drying; casein and D-glucose: Xilong Technology Co., Ltd.

[0041] Agilent 1260 infinity liquid chromatography-mass spectrometry (Agilent, USA).

[0042] 2. Optimization of the preparation conditions of the filler

[0043] Taking the in...

Embodiment 2

[0057] Example 2 Preparation of distiller's grain extract

[0058] 1. Experimental materials and instruments

[0059] Distiller's grains: sorghum as the main raw material, the residue after solid-state fermentation, obtained after freeze-drying; casein and D-glucose: Xilong Technology Co., Ltd.; water (DDW): prepared using WaterPro water purification system (Labconco Corp.).

[0060] Agilent 1260 infinity liquid chromatography-mass spectrometry (Agilent, USA).

[0061] 2. Optimization of extraction conditions

[0062] Taking the inhibition rate of carboxymethyl lysine as an index, a single factor experiment method was adopted,

[0063] Weigh 0.2 g of distiller's grains and add 10 mL of ethanol-water mixture (volume ratios of ethanol:water are 6:4, 7:3, 8:2, 9:1, 100% ethanol) as solvent, heat and stir at 60°C After extraction for 30 min, after solid-liquid separation, the extract was collected; 1.5 mL distiller’s grains extract was loaded onto a solid-phase extraction colum...

Embodiment 3

[0090] Example 3 Antioxidant properties of distiller's grains extract and inhibition rate to carboxymethyllysine

[0091] 1. Experimental materials and instruments

[0092] DPPH: Yuanye Biotechnology Co., Ltd.; FRAP kit: Beijing Banxia Technology Co., Ltd.; casein and D-glucose: Xilong Technology Co., Ltd.; disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate and aminoguanidine: J&K Science Ltd.; Agilent 1260 Infinity LC-MS (Agilent, USA).

[0093] 2. Experimental procedure

[0094] (1) Dissolve distiller's grain extracts C1, C2, and S1 in 5 mL of 70% acetone-water solvent respectively, and set aside.

[0095] (2) Scavenging ability for DPPH: Take 150 μL distiller’s grain extract and add to 2.85mL 6.6×10 -5 After mixing in mol / L DPPH solvent and reacting in the dark for 2 hours, measure the absorbance at 515nm. The inhibition rate of DPPH is:

[0096] Inhibition rate (%) = (1-A s / A c )×100%

[0097] Where As is the absorbance of the sample and Ac is t...

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Abstract

The invention provides a vinasse extract and a preparation method and application thereof. The extraction method comprises the following steps that in a protective atmosphere, trioctylphosphine-modified CdSe / ZnS quantum dots, glyoxal dimethacrylate, 4-vinyl pyridine and gallic acid are subjected to reaction in xylene when initiated by azodiisobutyronitrile, after purification and drying are conducted, filler for extracting an antioxidant component of vinasse is obtained, and a solid-phase extraction cartridge is prepared; the vinasse is added into ethyl alcohol-water mixed liquid for heating and stirring, and extraction liquid is collected; the homemade solid-phase extraction cartridge is used for extracting the vinasse component, and after drying is conducted, the vinasse extract is obtained. The vinasse extract obtained through the method contains a phenolic acid substance, and can be used as a food additive for inhibiting carboxy methyl lysine in a food processing process, novel application of the white liquor vinasse is extended, and a novel path is provided for resource utilization of the vinasse. The method for preparing the vinasse extract is simple and convenient in extraction process and low in cost, industrialized application is easy, and the vinasse can be subjected to mass treatment.

Description

technical field [0001] The invention belongs to the field of resource utilization of waste, and in particular relates to a preparation method of liquor lees extract. Background technique [0002] Advanced glycation end products (Advanced Glycation End Products, AGEs) are formed in the second reaction stage of MR, that is, the late stage, which is caused by the reaction of protein with aldose and non-enzymatic glycosylation or the reaction of lipid with aldose. A class of compounds formed by oxidation. Carboxymethyllysine (carboxymethyllysine) has no fluorescent properties, no cross-linking properties, and is acid-stable. Carboxymethyllysine is the most studied and most representative advanced glycation terminal The product, carboxymethyllysine, is often used as the primary target assay for the quantification of AGEs in food. [0003] Natural polyphenols have various biological activities such as anti-oxidation and anti-apoptosis, and have a great effect on promoting gastro...

Claims

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Application Information

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IPC IPC(8): A23L33/10
Inventor 刘慧琳王静王玉珍
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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