40 results about "Meyerozyma guilliermondii" patented technology

The invention discloses meyerozyma guilliermondii for controlling postharvest diseases of pome, and belongs to the field of biological prevention and treatment for postharvest diseases of fruits. The preservation number of the identified meyerozyma guilliermondii is CCTCC NO:M 2017270. The meyerozyma guilliermondii is activated, cultured and centrifuged to obtain thalli when used, and the thalli are diluted by sterile water to prepare bacterium suspension with the concentration of 1.0*10<8>bacteria / mL. The pome is soaked in the bacterium suspension for approximately 1 min, then is subjected to natural air drying, is placed in plastic baskets and is stored under room-temperature conditions after being sealed by preservative films. The meyerozyma guilliermondii has the advantages that the penicillosis incidence and the natural rotting rate of the pome treated by the meyerozyma guilliermondii are obviously reduced, accordingly, the meyerozyma guilliermondii can be used for preventing and treating the postharvest diseases of the pome instead of chemical fungicides, harm on human due to the chemical fungicides can be prevented, and the meyerozyma guilliermondii has an obvious economic benefit and an obvious social benefit.

The invention discloses a method for preparing bioethanol by taking sodium alginate or algae as active ingredients. Fermentation culture medium taking sodium alginate as a carbon source can be adopted, or the fermentation culture medium taking algae treatment liquor as a carbon source can be adopted, the algae treatment liquor is obtained by adopting the following method: mixing algae powder with water, and sterilizing; mixing algae powder with citric acid-sodium citrate buffer solution, regulating the pH value to be neutral with NaOH solution, adding cellulase which is 10% of the mass of algae powder, performing enzymolysis so that the cellulase is inactivated; mixing algae powder with aqueous sulfuric acid solution, and hydrolyzing. The method for preparing bioethanol comprises the steps of: inoculating 5% Meyerozyma guilliermondii strain seed solution into a sterilized fermentation culture medium, multiplying, then performing airtight anaerobic fermentation for 5-7 days at 28-32DEG C; distilling the fermented liquid to obtain bioethanol. According to the method, the bioethanol can be produced by utilizing marine algae resources; therefore, the commercial crops of grains and the like can be saved, cultivated land cannot be occupied and fresh water resources cannot be consumed; remaining solids after fermentation can serve as organic fertilizers, and the algae utilization rate can be improved; the method is simple and easy, and absolutely capable of carrying out industrial production.

The invention discloses Meyerozyma guilliermondii K1 and an application thereof in biological deodorization, and belongs to the technical field of biology. The preservation number of the Meyerozyma guilliermondii K1 is CCTCC NO: M 2020191. The strain is high in deodorization efficiency and free of biological danger, and a biological deodorization microbial agent prepared from the Meyerozyma guilliermondii K1 has obvious degradation effects on odors generated by restaurant and kitchen waste, and has relatively good application potential.

The invention discloses J-SS-CX which is meyerozyma guilliermondii with under the preservation number CGMCC NO.17240. The invention also discloses a meyerozyma guilliermondii suspension for controlling postharvest diseases of cherry tomato fruits. The preparation method of the meyerozyma guilliermondii suspension comprises the steps of inoculating meyerozyma guilliermondii J-SS-CX with an activated NYDA solid culture medium into an NYDB culture medium for one generation, then inoculating it into a NYDB culture medium containing 12% NaCl for culture, washing the obtained thalli with sterile distilled water and diluting it. The yeast preparation can effectively control postharvest diseases of fruits on the premise that a chemical bactericide is not used, and is convenient to operate, good in effect, low in cost, safe and environmentally friendly.

The invention discloses a method for preparing bioethanol by taking sodium alginate or algae as active ingredients. Fermentation culture medium taking sodium alginate as a carbon source can be adopted, or the fermentation culture medium taking algae treatment liquor as a carbon source can be adopted, the algae treatment liquor is obtained by adopting the following method: mixing algae powder with water, and sterilizing; mixing algae powder with citric acid-sodium citrate buffer solution, regulating the pH value to be neutral with NaOH solution, adding cellulase which is 10% of the mass of algae powder, performing enzymolysis so that the cellulase is inactivated; mixing algae powder with aqueous sulfuric acid solution, and hydrolyzing. The method for preparing bioethanol comprises the steps of: inoculating 5% Meyerozyma guilliermondii strain seed solution into a sterilized fermentation culture medium, multiplying, then performing airtight anaerobic fermentation for 5-7 days at 28-32DEG C; distilling the fermented liquid to obtain bioethanol. According to the method, the bioethanol can be produced by utilizing marine algae resources; therefore, the commercial crops of grains and the like can be saved, cultivated land cannot be occupied and fresh water resources cannot be consumed; remaining solids after fermentation can serve as organic fertilizers, and the algae utilization rate can be improved; the method is simple and easy, and absolutely capable of carrying out industrial production.

The invention relates to phytolacca acinosa fruit fermentation liquor and a preparation method thereof. According to the method, ripe phytolacca acinosa fresh fruits are used as a raw material, and eight kinds of mixed strains of Penicillium sublateritium, Golubevia pallescens, Aspergillus penicillioids, Aspergillus parvulus, Meyerozyma guilliermondii, Candida intermedia, Lodderomyces elongisporusand Aspergillus sydowii are taken as fermented strains, and secondary fermentation is carried out at a temperature of 18-32 DEG C. The preparation method is clear in fermentation strain and controllable in process. The prepared fermentation liquor is clear in chemical components and has remarkable anti-cancer activity.

The invention discloses a kind of Pichia quaternary yeast J-SS-CX, which is Meyerozyma guilliermondii, and the deposit number is CGMCC NO.17240. The invention also discloses a Pichia moniliformis suspension for controlling postharvest diseases of cherry tomato fruits. The preparation method is as follows: Pichia moniliformes J-SS activated in NYDA solid medium ‑CX was inoculated into NYDB medium for one generation, and then inoculated into NYDB medium containing 12% NaCl for culture, and the obtained cells were washed and diluted with sterile distilled water. The invention can realize effective control of postharvest diseases of fruits without using chemical fungicides, and the yeast preparation is convenient to operate, has good effect, low cost, safety and environment-friendly.

The invention discloses a Pichia guilliermondii (Meyerozyma guilliermondii) K1 and its application in biological deodorization, belonging to the field of biological technology. The deposit number of the Pichia guilliermondii K1 is CCTCC NO: M 2020191. The strain has high deodorizing efficiency and no biological hazard. The biological deodorizing bacteria agent made by using Pichia mongolica K1 has obvious degrading effect on the odor produced by kitchen waste, and has good application potential.

The invention discloses a ketoreductase mutant with improved enzymatic activity and an application thereof, belonging to the field of biotechnology. It is derived from the wild-type ketoreductase of Meyerozyma guilliermondii, and can convert ethyl 4-chloroacetoacetate to generate (R) ‑4‑chloro‑3‑hydroxybutyrate ethyl ester, the ketoreductase mutant has higher alcohol dehydrogenase activity than the wild-type sequence, and has more than 90% similarity with SEQ ID NO.8. The ketoreductase mutant of the present invention has obviously high specific enzyme activity, which is 2-10 times higher than that of the wild-type ketoreductase, the reaction conditions are mild, the equipment requirements are low, the production process does not require high temperature or cooling, and the energy consumption is low. Enzyme catalysis has high efficiency, specific selectivity, and convenient purification. In addition, most of the solvent in the reaction is water, and there is no need to add solvents such as butyl acetate to form a two-phase reaction system. The three wastes discharge is low, and the preparation process is environmentally friendly.

The subject invention provides improved methods for producing mannosylerythritol lipids (MEL) using yeasts not previously known to produce MEL. In particular, Meyerozyma guilliermondii (Pichia guilliermondii) is cultivated in a specially-tailored nutrient medium and under cultivation conditions such that the yeast unnaturally produces MEL and / or MEL-like molecules in greater amounts and at increased rates than when using standard MEL production with, for example, Pseudozyma aphidis. Yeast culture compositions are also provided, comprising yeast cells, growth medium, and high concentrations of MEL.

The invention relates to a biocontrol bacterial strain Y-1 for preventing and treating fungal diseases of grapes and its application in the prevention and treatment of grape diseases, belonging to the technical field of biological control of plant diseases. The bacterial strain is Meyerozyma guilliermondii Y‑1, and the strain preservation number is CCTCC NO: M2015814. The above-mentioned Pichia guilliermondii (Meyerozyma guilliermondii) Y-1 is used to prepare a biocontrol agent for preventing and treating grape diseases, which has a significant control effect on various pathogenic fungi. The active ingredient of the biocontrol preparation provided by the present invention, Pichia guilliermondii (Meyerozyma guilliermondii) Y‑1, has stable control effect, long-lasting effect and wide spectrum of disease prevention, and is environmentally safe and not suitable for drug resistance; The method has the advantages of fast speed, simple culture conditions, easy storage and industrial production; it can also improve the activity of defense-related enzymes in plants, and has good development and application prospects.

The invention discloses a ketoreductase mutant for producing (R)-4-chloro-3-hydroxybutyric acid ethyl ester, belonging to the field of biotechnology, which is derived from the wild-type ketoreductase of Meyerozyma guilliermondii and can convert Ethyl 4-chloroacetoacetate is converted to generate (R)-4-chloro-3-hydroxybutyric acid ethyl ester, which has higher alcohol dehydrogenase activity compared with the wild-type sequence, and the sequence of the ketoreductase mutant is SEQ ID NO.2, the enzyme activity of the ketoreductase mutant is at least 2-10 times higher than that of the wild-type ketoreductase. The ketoreductase mutants of the present invention and polynucleotides encoding such mutants can be prepared using methods commonly used by those skilled in the art. Mutants can be obtained by in vitro recombination encoding the enzyme, mutagenesis of polynucleotides, DNA shuffling, error-prone PCR, and directed evolution methods, among others.

A yeast strain and a method for the synthesis of 2,5-dihydroxymethylfuran using this strain are disclosed. The yeast strain is Meyerozyma guilliermondii SC 1103, which has been maintained in the China Center for Type Culture Collection (CCTCC, Wuhan, P.R. China) with an access No. of M2016144. The method for the synthesis of 2,5-dihydroxymethylfuran using this strain is described as follows: after pre-cultivation and cultivation, Meyerozyma guilliermondii SC 1103 cells are added into the buffer solutions containing 5-hydroxymethylfurfural and glucose; the biocatalytic reaction is conducted under designated conditions, thus affording 2,5-dihydroxymethylfuran. This disclosure has many advantages such as good selectivity, mild reaction conditions, environmental friendliness, high efficiency, and good yield.

The invention belongs to the technical field of industrial biology, and in particular relates to a screening method for nitrilase-producing yeast and its application in the biotransformation of nitrile compounds to prepare 3-hydroxypropionic acid. The present invention provides a method for screening yeast producing nitrilase. For the first time, a yeast strain 3H2-2 capable of transforming 3-hydroxypropionitrile into 3-hydroxypropionic acid has been screened, combined with morphology, physiological and biochemical characteristics and 18S and ITS sequencing analysis, it was identified as Pichia guilliermondii (Meyerozyma guilliermondii). The strain has been deposited in the General Microorganism Center of China Committee for Culture Collection of Microorganisms (preservation number CGMCC No. 12935). Adding 3% glucose as an energy source, using the free cells of the strain to carry out transformation experiments on 3-hydroxypropionitrile, can completely convert 500mM 3-hydroxypropionitrile, and the cumulative concentration of 3-hydroxypropionate can reach 216.3mM. Pichia mongolica CGMCC 12935 has the characteristics of strong vitality and outstanding nitrile conversion ability, and a new process has been established for the synthesis of 3-hydroxypropionic acid. This process has the characteristics of mild reaction conditions, low energy consumption, and environmental friendliness.