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Application of meyerozyma guilliermondii in aspect of degrading antibiotics

A technology of antibiotics and yeast, applied in climate change adaptation, food science, etc., can solve the problems of less degradation technology of sulfonamide antibiotics, secondary pollution of meat and bone meal, and limited use range, etc., to achieve low cost, reduce pollution, and improve utilization rate Effect

Active Publication Date: 2020-08-14
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The γ-radiation degradation method requires special radiation equipment, which has great hidden dangers to public safety, and the scope of application is strictly limited
The degradation efficiency of ultrasonic method is low
The palladium catalyst method and Fe +3 The carrier oxidative degradation method needs to add exogenous chemicals to the matrix. At the same time, the catalytic oxidation process will oxidize nutrients such as protein into harmful substances, which will cause secondary pollution to meat and bone meal
Microbial degradation is a low-cost and mild antibiotic degradation method, but there are relatively few degradation technologies for sulfa antibiotics, especially the technology suitable for the degradation of sulfa antibiotics in meat and bone meal of sick animals has not been reported yet

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Degradation experiment of Pichia montaglia sulfadiazine

[0032] 1. Experimental method

[0033] The present invention detects the residual amount of sulfadiazine with LC / MS method, calculates the degradation rate, and concrete operation is:

[0034] Mass spectrometry conditions: spray voltage: 4.5kv; atomization flow: 3.0L / min; drying gas flow: 15L / min; collision pressure (CID): 230kPa.

[0035] Liquid chromatography conditions: chromatographic column: Gold C18, 5 μm, 150mm×2.1mm (inner diameter); mobile phase: methanol A; ammonium acetate 5mmol / L (0.1% formic acid) aqueous solution B, see Table 1 for elution gradient, flow rate: 300 μL / min; column temperature: 30°C; injection volume: 10 μL.

[0036] Table 1 Liquid chromatography mobile phase gradient elution for separating drugs

[0037]

[0038] Dissolve sulfadiazine in an aqueous solution, dilute it with 5°Be wort to a final concentration of sulfadiazine of 25mg / L, 5mg / L, and 1mg / L, set 3 paralle...

Embodiment 2

[0041] Example 2 Degradation experiment of sulfadiazine in meat and bone meal of sick animals

[0042] Mix the diseased animal meat and bone meal with 3 times of water, add 1.5% molasses of the total weight, adjust the pH to 6.8, and prepare a fermentation medium, add it to the Erlenmeyer flask, and inoculate it into the fermentation medium after sterilization. Add 3% Pichia monteritiera seed liquid, and ferment for 3 days under the condition of 28°C.

[0043] Take 0.2 g of the fermentation substrate on a nutrient agar plate containing Escherichia coli indicator bacteria, measure the diameter of the inhibition zone, and use the unfermented substrate as a blank control to compare the degradation of sulfadiazine before and after fermentation. Such as figure 1 As shown, the inhibitory effect of unfermented meat and bone meal on Escherichia coli is obvious, indicating that the antibiotic residue in meat and bone meal is relatively high, which can inhibit the growth of Escherichia...

Embodiment 3

[0045] Example 3 Degradation of Sulfadiazine in Meat and Bone Meal of Sick Animals

[0046] Mix the diseased animal meat and bone powder with 4 times of water, add 3% molasses of the total weight, adjust the pH to 5.5, and prepare a fermentation medium. After sterilization, add 2% Ji Yemeng to the fermentation medium Pichia pastoris seed liquid was fermented at 25°C for 4 days.

[0047]The measurement method is as in Example 2, and the results show that the residual amount of sulfadiazine in the meat and bone powder of sick animals before fermentation is 8.08 mg / kg, the content of sulfadiazine at the end of fermentation is 0.94 mg / kg, the degradation rate of sulfadiazine is 88.3%, and the degradation rate of protein was 2.6%.

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Abstract

The invention discloses an application of meyerozyma guilliermondii in the aspect of degrading antibiotics or an application of the meyerozyma guilliermondii in the aspect of improving the degradationremoval rate of the antibiotics. First research finds that the meyerozyma guilliermondii can significantly degrade the antibiotics, especially sulfonamide antibiotics; and when the meyerozyma guilliermondii is applied to diseased animal meat and bone meal, a result shows that the degradation rate of sulfadiazine in the diseased animal meat and bone meal is close to 90%, and the degradation rate of protein is only about 2%, so that the utilization rate of the diseased animal meat and bone meal is greatly improved, and meanwhile the pollution of the antibiotics to the environment is reduced.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and veterinary medicine. More specifically, it relates to the application of Pichia mongolica in degrading antibiotics. Background technique [0002] At present, the harmless treatment of sick livestock and poultry has formed a considerable scale. Because it is a national requirement, almost all counties and cities in the country have established corresponding harmless treatment institutions. The most widely used treatment method is to sterilize and humidify the diseased and dead livestock and poultry. The waste residue after chemical treatment will be dried to obtain a large amount of meat and bone meal, which contains about 40% to 50% of protein and about 30% of minerals, which is a good nutritional resource for feeding. However, due to the large amount of antibiotics used in the treatment of sick animals, the antibiotic residues in meat and bone meal seriously exceeded the standard, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
CPCA23L5/28Y02A40/70
Inventor 孙力军孙东方王雅玲房志家邓旗
Owner GUANGDONG OCEAN UNIVERSITY
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