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Method for preparing bioethanol by taking sodium alginate or algae as active ingredients

A bioethanol, sodium alginate technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as lack of original biological genetic modification methods, difficult control of genetic and metabolic processes, and lack of stability. , to achieve the effect of improving the utilization rate of seaweed, saving economic crops, and the method is simple and easy to implement

Inactive Publication Date: 2014-03-05
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of stability in the case of industrial fermentation, the difficulty of controlling genetic and metabolic processes, and the lack of methods for genetically modifying this original organism, it seems unlikely that the engineered bacteria will be used in large-scale fermentation to produce ethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Get 100g and use sodium alginate as the fermentation medium prepared by carbon source, the composition of this fermentation medium is as follows: (NH 4 ) 2 SO 4 10.8g / L, KH 2 PO 4 5.0g / L, MgSO 4 ·7H 2 O1.1g / L, sodium alginate 20g / L, pH = 6, sterilized at 121°C for 15 minutes, cooled to room temperature; add Meyerozyma guilliermondii strain seed solution to the above liquid at an inoculation mass ratio of 5%, and mix well , and then propagated at 30°C, 150rpm for 24 hours; at 28-32°C, seal the anaerobic fermentation culture, connect the airway, and insert the other end into the water to prevent air from entering the fermentation vessel. The fermentation time is 5 days, and the pH value is not adjusted during the fermentation process, but the gas needs to be discharged; the separated liquid is distilled to obtain 15.4 g of ethanol, and the yield is 0.154 g ethanol / g sodium alginate.

Embodiment 2

[0028] Remove impurities on the surface of dried kelp, crush it with a pulverizer, and pass it through a 100-mesh sieve; take 20g of kelp powder, mix it with 600mL (30 times) of water, sterilize at 110°C-115°C for 15min, and obtain the seaweed treatment solution after cooling; use kelp Hydrolyzate instead of sodium alginate to prepare fermentation medium ((NH 4 ) 2 SO 4 10.8g / L, KH 2 PO 4 5.0g / L, MgSO 4 ·7H 2 O1.1g / L, the above-mentioned kelp treatment solution), adjust the pH value of the fermentation medium to 5 with 2mol / L HCl solution; sterilize at 115°C for 15min, cool to room temperature, and insert Meyerozyma guilliermondii into the liquid with 5% inoculum mass ratio Mix the seed liquid of the bacteria evenly, and then carry out single-strain propagation and culture at 30°C and 150rpm for 24 hours; connect the airway, insert the other end into the water, and then conduct sealed anaerobic fermentation at 28-32°C for 5 days, the fermentation process The pH value is ...

Embodiment 3

[0030] Remove impurities on the surface of dried kelp, crush it with a pulverizer, and pass through a 100-mesh sieve; mix 20g of kelp powder with 600mL (30 times) of water, cook at 90-100°C for 20-30min, and cool to obtain a seaweed treatment solution; hydrolyze with kelp substance instead of sodium alginate, the preparation of fermentation medium ((NH 4 ) 2 SO 4 10.8g / L, KH 2 PO 4 5.0g / L, MgSO 4 ·7H 2 (01.1g / L, the above-mentioned kelp treatment solution), adjust the pH of the fermentation medium to 5 with 2mol / L HCl, sterilize at 115°C for 15min, and insert Meyerozyma guilliermondii bacteria into the fermentation broth with a 5% inoculum mass ratio after cooling to room temperature species and the seed liquid of Saccharomyces cerevisiae, their ratio is 4:1, mix well, and then carry out the mixed strain propagation and culture at 30°C, 150rpm for 24h; , sealed anaerobic fermentation for 5 days, the pH value is not adjusted during the fermentation process, but the gas ne...

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PUM

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Abstract

The invention discloses a method for preparing bioethanol by taking sodium alginate or algae as active ingredients. Fermentation culture medium taking sodium alginate as a carbon source can be adopted, or the fermentation culture medium taking algae treatment liquor as a carbon source can be adopted, the algae treatment liquor is obtained by adopting the following method: mixing algae powder with water, and sterilizing; mixing algae powder with citric acid-sodium citrate buffer solution, regulating the pH value to be neutral with NaOH solution, adding cellulase which is 10% of the mass of algae powder, performing enzymolysis so that the cellulase is inactivated; mixing algae powder with aqueous sulfuric acid solution, and hydrolyzing. The method for preparing bioethanol comprises the steps of: inoculating 5% Meyerozyma guilliermondii strain seed solution into a sterilized fermentation culture medium, multiplying, then performing airtight anaerobic fermentation for 5-7 days at 28-32DEG C; distilling the fermented liquid to obtain bioethanol. According to the method, the bioethanol can be produced by utilizing marine algae resources; therefore, the commercial crops of grains and the like can be saved, cultivated land cannot be occupied and fresh water resources cannot be consumed; remaining solids after fermentation can serve as organic fertilizers, and the algae utilization rate can be improved; the method is simple and easy, and absolutely capable of carrying out industrial production.

Description

technical field [0001] The invention relates to a method for preparing bioethanol, in particular to a method for preparing bioethanol by using seaweed and seaweed extract as raw materials. Background technique [0002] At present, oil resource reserves are on the verge of depletion, and bioethanol has become a more effective alternative product. Bioethanol refers to the production of ethanol from biomass rich in carbohydrates through microbial fermentation, which can be added to gasoline to make mixed fuels. However, most of the processed fuel ethanol uses food or economic crops such as corn, wheat, soybean, rapeseed, sugarcane, etc. From the analysis of energy input and output, this structure is uneconomical and has caused worldwide controversy . Other raw materials are difficult to form large-scale industrial production capacity due to the limitations of cultivated land and technology. Marine algae resources are ideal raw materials for bioethanol production due to their ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/06C12R1/86C12R1/645
CPCY02E50/17Y02E50/10
Inventor 张雯
Owner YANSHAN UNIV
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