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Extract having effects of being preservative and fresh-keeping and application

A technology of extracts and functions, applied in the field of extracts with antiseptic and fresh-keeping functions, can solve the problems that the antibacterial activity of natural material components has not been reported, and achieve the effect of avoiding food safety and pollution

Inactive Publication Date: 2018-03-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current research on the extract of peony flower is mainly focused on its antioxidant activity and component analysis, and the antibacterial activity of its natural components has not been reported.

Method used

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  • Extract having effects of being preservative and fresh-keeping and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] After crushing and sieving the peony flowers, use the ethanol extraction method to reflux extraction for 2 hours under the conditions of ethanol volume fraction of 95%, temperature of 70°C-80°C, and solid-liquid ratio of 1:10. After suction filtration, it was concentrated with a rotary evaporator, freeze-dried to make a freeze-dried powder, and stored in a sealed refrigerator at 4°C for later use.

Embodiment 2

[0015] The antibacterial effect of Paeoniae officinale extract was detected by the filter paper diffusion method. The filter paper sheet (diameter 6mm) was wetted with different concentrations of Paeoniae officinale extract, and placed in the place where the test bacteria (concentration of 10 mm) had been evenly coated. 6 cfu / mL), a filter paper soaked in sterile saline was used as a control. Bacteria were cultured at 37°C for 24h, yeast at 30°C for 48h, and the size of the inhibition zone was measured. The minimum inhibitory concentration of peony flower extract to the tested bacteria was also determined by the filter paper diffusion method, and the critical concentration at which the inhibition zone appeared was taken as the minimum inhibitory concentration. The results are shown in Table 1.

[0016] Table 1 Antibacterial effect and minimum inhibitory concentration of Paeoniae officinalis flower extract

[0017]

Embodiment 3

[0019] In Bacillus subtilis (at a concentration of 10 6 cfu / mL) into the bacterial liquid of Paeoniae alba, so that the final concentration is 2, 4 mg / mL, respectively at 0, 1, 5, 10, 15, 20, 25 min, measure the conductance of the bacterial liquid with a conductivity meter Taking the bacterial liquid treated with peony flower extract as the control, after Bacillus subtilis was treated with the peony flower extract, the conductivity of the bacterial liquid increased rapidly within 1 min, and the higher the concentration of the extract, the greater the conductivity value .

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Abstract

The invention relates to an extract having the effects of being preservative and fresh-keeping and an application of the extract. The extract is selected from peony, through ethanol / aqueous extraction, and then through freeze drying, dry powder is made; the extract has obvious restraining effect on bacillus subtilis, Xanthomonas campestris pv.campestris, Meyerozyma guilliermondii, Candida tropicalis and Pichia kluyveri; after thalli is treated with the extract, the electrical conductivity of bacterial liquid is instantaneously and rapidly increased within 1min, and the damage capacity to cellfilms is high; and fruits and vegetables are soaked or sprayed with the extract, the rotting rate and the weight loss rate of the fruits and the vegetables can be reduced, the loss of soluble solids and Vc is reduced, the extract has the effects of being preservative and fresh-keeping and can replace a chemical fresh keeping agent for preservation of the fruits and the vegetables, and the problemsthat a chemical preservation agent pollutes environment, and the food is unsafe, are solved.

Description

technical field [0001] The invention relates to the field of fresh-keeping of fruits and vegetables, and relates to an extract with antiseptic and fresh-keeping functions and an application thereof. Background technique [0002] my country has always been a big fruit and vegetable producing country, especially in recent years, the fruit and vegetable industry has developed rapidly, but due to the seasonal and regional restrictions of fruit and vegetable production, it is easy to cause mechanical damage to fruits and vegetables in the process of picking, transportation and storage, which becomes a window for microbial invasion. It is one of the main causes of fruit and vegetable spoilage loss. As food safety is getting more and more attention, the use of natural antibacterial substances to replace the currently used chemical fungicides to preserve fruits and vegetables is also popular because of their unique naturalness and safety. Therefore, extracting natural antibacterial ...

Claims

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Application Information

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IPC IPC(8): A23B7/154
Inventor 诸葛斌李雯霞于司达宗红陆信曜
Owner JIANGNAN UNIV
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