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Meyerozyma guilliermondii and application thereof

A yeast, Y-1 technology, applied to Pichia mongolica Y-1 and its application in the prevention and treatment of grape fungal diseases, can solve the problems of high cost, high pesticide residues, and difficult control of fungal diseases, etc. Achieve the effect of long duration, reduce the frequency and amount of use, and broad-spectrum characteristics of duration

Active Publication Date: 2018-04-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of difficult prevention and control of main fungal diseases in grape production and storage period in current agricultural production, high cost, increasingly prominent resistance to chemical pesticides, and high pesticide residues. Effective control of fungal diseases

Method used

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  • Meyerozyma guilliermondii and application thereof
  • Meyerozyma guilliermondii and application thereof
  • Meyerozyma guilliermondii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation and strain identification of Pichia pastoris (Meyerozymaguilliermondii) Y-1 described in Example 1

[0029] 1.1 Isolation and screening of strains

[0030]Mature and healthy Kyoho grapes were collected from the commercialized vineyards in Yantai, Shandong, and strains were isolated from the surface of the grapes. The conventional gradient dilution coating separation method was used, and PDA medium (containing streptomycin sulfate) and YPD agar medium (containing streptomycin sulfate) were used to isolate the strains. ) were cultured at 28°C, and the colonies with obvious differences in colony morphology were picked, purified and preserved on YPD agar medium, and the primary screening and multiple re-screening of antagonistic bacteria were carried out with grape white rot as the target pathogen, and finally obtained A highly active antagonistic yeast strain named Y-1.

[0031] The above-mentioned PDA medium used has the following components and formula: weigh ...

Embodiment 2

[0038] Example 2 The inhibitory effect of Pichia pastoris (Meyerozyma guilliermondii) Y-1 on various plant pathogenic fungi

[0039] Different concentrations of Pichia guilliermondii (Meyerozyma guilliermondii) Y-1 cells had significant inhibitory effects on botrytis cinerea and grape anthracnose, and PDA was added with 10 7 cfu mL -1 The most significant inhibitory effect was strain Y-1 ( image 3 with Figure 4 ). In addition, 10 7 cfu mL -1 Pichia mongolica Y-1 cells are also effective against grape white rot, grape penicillium, grape brown rot, apple ringworm, apple tree rot, pear tree rot, apple fruit spot disease and Apple mold core pathogen and many other fruit tree pathogenic fungi have significant inhibitory effect, showing good broad-spectrum antibacterial properties. The results are shown in Table 1.

[0040] Table 1 Inhibitory effect of Meyerozyma guilliermondii Y-1 on the main pathogenic fungi of fruit trees

[0041]

[0042]

[0043]

[0044] Note...

Embodiment 3

[0046] Example 3 The control effect of Pichia pastoris (Meyerozyma guilliermondii) Y-1 bacterial suspension spray application on grape diseases

[0047] Inoculate the Y-1 seed fermentation liquid of Pichia guilliermondii into YPD liquid medium, and culture it with constant temperature and shaking at 30°C for 48 hours to obtain the cell culture liquid of the Y-1 strain, and adjust the concentration to 10 6 cfu mL -1 , 10 7 cfu mL -1 and 10 8 cfu mL -1 bacterial suspension. Botrytis cinerea, grape anthracnose, grape white rot, grape Penicillium and grape brown rot were activated and cultured on PDA for 3 days, and fungus cakes with a diameter of 5 mm were taken from the edge of the colony for later use. Select Kyoho grapes with uniform and healthy fruit size, sterilize the surface with 75% alcohol, and then dry them at room temperature. At the equator of the grapes, puncture to cause micro-wounds, one wound for each fruit, each wound has a diameter of 0.3mm and a depth of ...

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Abstract

The invention relates to a biocontrol strain Y-1 for preventing and controlling fungal diseases of grapes and application of the biocontrol strain Y-1 in prevention and control of grape diseases and belongs to the technical field of biological prevention and control of plant diseases. The strain is Meyerozyma guilliermondii Y-1 with a preservation number of CCTCC (China Center For Type Culture Collection) NO:M2015814. The Meyerozyma guilliermondii Y-1 is used for preparing a biocontrol preparation for preventing and controlling the grape diseases, and the biocontrol preparation has an obviousprevention and control effect on multiple pathogenic fungi. The Meyerozyma guilliermondii Y-1, provided by the invention, as an active ingredient of the biocontrol preparation, has the characteristicsof stable prevention and control effect, long persistence and wide disease control spectrum as well as has the properties of environment friendliness, safety and low possibility of generating pesticide resistance; the strain has high propagation speed, is cultured under simple culture conditions and is easy for storage and industrial production; defense related enzyme activity in plant bodies canbe improved, and a good development and application prospect is realized.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to Pichia mongoliana Y-1 and its application in preventing and controlling grape fungus diseases. Background technique [0002] Grape is a colorful, delicious and nutritious fruit, which is an important source of nutrition and material basis for people. my country's grape cultivation area and output rank among the top in the world, among which Shandong grape production accounts for about 16% of the country's total output. my country's grape production is dominated by fresh varieties and wine varieties, and its output accounts for 35% of the world's total grapes, ranking second in the world. However, grape diseases seriously restrict the healthy and sustainable development of the grape industry. The diseases caused by fungal infection in grape production and post-harvest links have brought huge economic damage to the grape industry. Effective prevention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/04A01P3/00C12R1/84
CPCA01N63/30C12N1/16C12N1/165C12R2001/84
Inventor 王彩霞孟璐璐李桂舫张清明李保华
Owner QINGDAO AGRI UNIV
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