The invention provides a rectal cancer operation training model manufacturing method and a rectal cancer operation training model, and relates to the technical field of medical treatment. According to the manufacturing method of the rectal cancer operation training model, the stomach tissue is made into a barrel shape, one end simulates the anus end, the mucosa layer at the other end is connected with the rectum module to simulate the rectum of the human body, the serosal layer is fixed to the peritoneum, the serosal layer and the mucosa layer form the presacral space, and the stomach tissue is of a double-layer membrane structure and is close to the rectum in texture; the preparation method disclosed by the invention is simple and quick, the materials are convenient to take, and the cost is low. The rectal cancer surgery training model provided by the invention is manufactured by taking in-vitro stomach tissue, rectal tissue and peritoneum as raw materials, fully simulates the human rectal environment, can be used for training rectal cancer surgery, ensures feedback of training operation touch and strength, enables doctors to obtain real and convenient skill training, and improves surgical skills; meanwhile, the training environment is simplified, and investment of sites and labor is saved.