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A strain of Pichia monteriti and its application

A yeast and strain technology, applied to Pichia mongolica Y-1 and its application in the prevention and treatment of grape fungal diseases, can solve the problems of high cost, high pesticide residues, and difficult control of fungal diseases, etc., and achieve sustainable Long-term effect, reduce the frequency and amount of use, and the effect of broad-spectrum characteristics of long-term effect

Active Publication Date: 2020-06-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of difficult prevention and control of main fungal diseases in grape production and storage period in current agricultural production, high cost, increasingly prominent resistance to chemical pesticides, and high pesticide residues. Effective control of fungal diseases

Method used

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  • A strain of Pichia monteriti and its application
  • A strain of Pichia monteriti and its application
  • A strain of Pichia monteriti and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation and strain identification of Pichia pastoris (Meyerozymaguilliermondii) Y-1 described in Example 1

[0029] 1.1 Isolation and screening of strains

[0030] Mature and healthy Kyoho grapes were collected from the commercialized vineyards in Yantai, Shandong, and strains were isolated from the surface of the grapes. The conventional gradient dilution coating separation method was used to separate the strains with PDA medium (containing streptomycin sulfate) and YPD agar medium (containing streptomycin sulfate). ) were cultured at 28°C, and the colonies with obvious differences in colony morphology were picked, purified and preserved on YPD agar medium, and the primary screening and multiple re-screening of antagonistic bacteria were carried out with grape white rot bacteria as the target pathogen, and finally obtained A highly active antagonistic yeast strain named Y-1.

[0031]The above-mentioned PDA medium used has the following components and formula: weigh ...

Embodiment 2

[0038] Example 2 The inhibitory effect of Pichia pastoris (Meyerozyma guilliermondii) Y-1 on various plant pathogenic fungi

[0039] Different concentrations of Pichia guilliermondii (Meyerozyma guilliermondii) Y-1 cells had significant inhibitory effects on botrytis cinerea and grape anthracnose, and PDA was added with 10 7 cfu·mL-1 strain Y-1 had the most significant inhibitory effect ( image 3 with Figure 4 ). In addition, 10 7 cfu·mL-1 of Pichia mongolica Y-1 cells are also effective against grape white rot, grape penicillium, grape brown rot, apple ringworm, apple tree rot, pear tree rot, apple Fruit spot disease pathogen and apple mold heart pathogen and other pathogenic fungi of fruit trees have significant inhibitory effect, showing good broad-spectrum antibacterial properties. The results are shown in Table 1.

[0040] Table 1 Inhibitory effect of Meyerozyma guilliermondii Y-1 on the main pathogenic fungi of fruit trees

[0041]

[0042]

[0043] Note: Th...

Embodiment 3

[0044] Example 3 The control effect of Pichia pastoris (Meyerozyma guilliermondii) Y-1 bacterial suspension spray application on grape diseases

[0045] Inoculate the Y-1 seed fermentation broth of Pichia pastoris (Meyerozymaguilliermondii) in the YPD liquid medium, and culture it at a constant temperature at 30°C for 48 hours to obtain the cell culture fluid of the Y-1 strain, and adjust the concentration to 10 6 cfu mL-1, 10 7 cfu·mL-1 and 10 8 cfu·mL-1 bacterial suspension. Botrytis cinerea, grape anthracnose, grape white rot, grape Penicillium and grape brown rot were activated and cultured on PDA for 3 days, and fungus cakes with a diameter of 5 mm were taken from the edge of the colony for later use. Select Kyoho grapes with uniform and healthy fruit size, sterilize the surface with 75% alcohol, and then dry them at room temperature. At the equator of the grapes, puncture to cause micro-wounds, one wound for each fruit, each wound has a diameter of 0.3mm and a depth of...

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Abstract

The invention relates to a biocontrol bacterial strain Y-1 for preventing and treating fungal diseases of grapes and its application in the prevention and treatment of grape diseases, belonging to the technical field of biological control of plant diseases. The bacterial strain is Meyerozyma guilliermondii Y‑1, and the strain preservation number is CCTCC NO: M2015814. The above-mentioned Pichia guilliermondii (Meyerozyma guilliermondii) Y-1 is used to prepare a biocontrol agent for preventing and treating grape diseases, which has a significant control effect on various pathogenic fungi. The active ingredient of the biocontrol preparation provided by the present invention, Pichia guilliermondii (Meyerozyma guilliermondii) Y‑1, has stable control effect, long-lasting effect and wide spectrum of disease prevention, and is environmentally safe and not suitable for drug resistance; The method has the advantages of fast speed, simple culture conditions, easy storage and industrial production; it can also improve the activity of defense-related enzymes in plants, and has good development and application prospects.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to Pichia mongoliana Y-1 and its application in preventing and controlling grape fungus diseases. Background technique [0002] Grape is a colorful, delicious and nutritious fruit, which is an important source of nutrition and material basis for people. my country's grape cultivation area and output rank among the top in the world, among which Shandong grape production accounts for about 16% of the country's total output. my country's grape production is dominated by fresh varieties and wine varieties, and its output accounts for 35% of the world's total grapes, ranking second in the world. However, grape diseases seriously restrict the healthy and sustainable development of the grape industry. The diseases caused by fungal infection in grape production and post-harvest links have brought huge economic damage to the grape industry. Effective prevention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A01N63/32A01P3/00C12R1/645
CPCA01N63/30C12N1/16C12N1/165C12R2001/84
Inventor 王彩霞孟璐璐李桂舫张清明李保华
Owner QINGDAO AGRI UNIV
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