Method for keeping flavor of seasoning bag for honey-stewed barbecue pork through microbial fermentation
A microbial fermentation and flavor-encapsulating technology, which is applied in the field of microbial fermentation and food processing, can solve the problems of honey sauce char siew losing flavor, lack of taste, and unevenness
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[0038] Ⅰ Preparation method of conditioning material
[0039] A preparation method of a conditioning material made by microbial fermentation, comprising the following steps:
[0040] S1: Meyerozyma guilliermondii strain preparation:
[0041] It is preferably prepared from sea cucumbers, specifically: clean the sea cucumbers with sterile water, place them in pure sea water, and grind them into a uniform homogenate in a sterile environment; coat the homogenate on marine agar and heat it at 35-38°C Incubate for 70 to 75 hours, select the mucus colony and confirm it through detection, and obtain the strain of Meyerozyma guilliermondii. Among them, the preparation method of marine agar is: 2-5g of peptone, 1-3g of yeast extract, 0.1-0.2g of ferric phosphate, 15-20g of agar, and 1000mL of aged seawater (natural seawater placed in the dark for 1 month) , where the pH was adjusted to 7.6.
[0042] The strain number of Meyerozyma guilliermondii is ATCC20283.
[0043] S2: After cult...
Embodiment 1
[0048] A preparation method of a conditioning material made by microbial fermentation, comprising the following steps:
[0049] S1: Preparation of Meyerozyma guilliermondii strains. Sea cucumbers were cleaned with sterile water, placed in pure seawater, and ground into a homogeneous homogenate in a sterile environment; the homogenate was spread on marine agar and incubated at 38°C for 73 Hours, the mucus colony was selected and determined through detection to obtain the Meyerozyma guilliermondii bacterial classification. Among them, the preparation method of marine agar is as follows: 2g of peptone, 3g of yeast extract, 0.15g of ferric phosphate, 15g of agar, and 1000mL of aged seawater (natural seawater placed in the dark for 1 month) are prepared, and the pH is adjusted to 7.6.
[0050] S2: After culturing the above-mentioned Meyerozyma guilliermondii strain on the culture medium at 40°C for 46 hours, the number of effective viable bacteria was 10 9 cfu / ml of Meyerozyma gui...
Embodiment 2
[0054] A preparation method of a conditioning material made by microbial fermentation, comprising the following steps:
[0055] S1: Preparation of Meyerozyma guilliermondii strains. Sea cucumbers were cleaned with sterile water, placed in pure seawater, and ground into a homogeneous homogenate in a sterile environment; the homogenate was spread on marine agar and incubated at 37°C for 70 Hours, the mucus colony was selected and determined through detection to obtain the Meyerozyma guilliermondii bacterial classification. Among them, the preparation method of marine agar is as follows: 5g of peptone, 2g of yeast extract, 0.1g of ferric phosphate, 20g of agar, and 1000mL of aged seawater (natural seawater placed in the dark for 1 month) are prepared, and the pH is adjusted to 7.6.
[0056] S2: After the above-mentioned Meyerozyma guilliermondii strain was cultured on the medium at 39°C for 44 hours, the effective number of viable bacteria was 10 9 cfu / mL of Meyerozyma guillierm...
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