Selective synthesis method for 2, 5-dihydroxymethylfuran

Abstract

The invention discloses a selective synthesis method for 2, 5-dihydroxymethylfuran. The method includes the steps of: (1) conducting domestication, activation and immobilization on meyerozyma guilliermondii SC1103 to obtain immobilized cells; (2) adding the immobilized cells into a buffer solution containing 50-350mM 5-hydroxymethylfurfural and cosubstrates, and carrying out reaction at 10-40DEG Cunder a stirring condition to obtain 2, 5-dihydroxymethylfuran. Specifically, the molar concentration of reducing sugar in the cosubstrates is 0.3-1.0 times that of 5-hydroxymethylfurfural. The immobilized biocatalyst provided by the invention has high tolerance to the substrate, can achieve efficient and high selective catalysis of high concentration substrate reduction to synthesize a target product, achieve a high product space time yield, and is beneficial to simplifying the subsequent separation and purification process of the target product.

Description

technical field [0001] The invention belongs to the field of biocatalysis and biomanufacturing, and in particular relates to a method for preparing 2,5-dimethylfuran by using immobilized yeast cells to catalyze the selective reduction of 5-hydroxymethylfurfural. Background technique [0002] In recent years, the development and utilization of bio-based energy and platform compounds have gradually attracted the attention of academia and industry. 5-hydroxymethylfurfural (5-hydroxymethylfurfural, HMF) is an important bio-based platform compound (Green Chem., 2010, 12, 539), which can be prepared by carbohydrate dehydration. HMF molecules have multiple active groups such as hydroxyl groups, aldehyde groups and carbon-carbon double bonds, so they are easily converted into various high value-added chemicals. 2,5-Dihydroxymethylfuran (2,5-dihydroxymethylfuran, DHMF) is a selective reduction product of aldehyde groups in HMF molecules, and has important application value in the fi...

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Problems solved by technology

However, the catalytic system reported by He et al. is too complex, requiring the addition of a large number of co-substrates and other additives (200mM glucose, 400mM xylose, 250mM glutamic acid, 1.5mM Mg 2+ , 40mM β-cyclodextrin and 12.5mM cetyltrimethylammonium bromide), which not only greatly reduces the economy and practicability of the process, but also increases the difficulty of subsequent product separation and purification

At the same time, the reaction time of the biocatalytic process is too long, requiring 72h

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