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Complex microbial agent for degrading saponin and tannin

A technology of composite microbial agent and microbial agent, applied in the direction of microorganisms, microorganisms, methods based on microorganisms, etc., to achieve the effects of easy cultivation, convenient operation, and low cost

Active Publication Date: 2019-01-04
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, few studies have reported microbial mixtures that can simultaneously degrade tannins and saponins

Method used

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  • Complex microbial agent for degrading saponin and tannin
  • Complex microbial agent for degrading saponin and tannin
  • Complex microbial agent for degrading saponin and tannin

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Embodiment Construction

[0028] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0029] (1) Cultivation and preservation of bacterial strains:

[0030] 1. Medium

[0031] Tryptone 10g, yeast extract powder 5g, sodium chloride 10g, and agar 15.0g, pH 7.

[0032] Potato extract powder 10g, glucose 20g, agar 15.0g, pH 7.0-7.2.

[0033] 2. Bacillus amyloliquefaciens S301

[0034] Put Bacillus amyloliquefaciens S301 into LB broth medium, culture at 30°C and shake at 150r / min for 3 days; then prepare the slant of LB agar medium test tube, after solidification, insert the strain into the slant of the test tube, culture for 3 days, and wait for a long time Fill the slope and store in a 4-5°C refrigerator.

[0035] 3. Meyerozyma guilliermondii S302

[0036]Put Meyerozyma guilliermondii S302 into potato dextrose broth culture medium, 30°C, 150r / min shaking cultu...

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Abstract

The invention provides a complex microbial agent for degrading saponin and tannin. The agent is composed of the microbial agents for degrading the saponin of bacillus amyloliquefaciens S301 and meyerozyma guilliermondii and the microbial agents for degrading the tannin of aspergillus awamori T301, wherein the volume ratio of the three microbial agents is (1-5):(1-5):(3:5). The complex microbial agent for degrading the saponin and tannin has the advantages that the complex microbial agent has the ability to degrade the saponin and the tannin, especially the saponin and tannin in sasanqua shell,sasanqua dregs and wood bits of pinus massoniana, and the degradation rate is 68% or above, the application of the complex microbial agent in the degradation of plant materials containing the saponin, tannin and the like has good prospects, and the application of the material rich in the saponin and tannin is greatly expanded.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, in particular to a composite microbial bacterial agent capable of degrading saponin and tannin. Background technique [0002] Saponin is a kind of component with relatively complex structure, which is composed of saponin and sugar, uronic acid or other organic acids. Saponins are widely distributed in the plant kingdom and are distributed in both monocotyledonous and dicotyledonous plants. There are more and more reports about saponin poisoning. For example, kidney beans, also known as green beans, are a kind of food commonly used in my country. Improper consumption of kidney beans often causes poisoning. This is related to the fact that kidney beans contain a variety of antinutrients. one. Saponins, bitter and pungent, are irritating to mucous membranes and the heart, and are strong hemolytic agents. Tea saponin (TS) is a general term for a class of oleanane-type pentacyclic triterpen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C12R1/07C12R1/665C12R1/645
CPCC05F5/002C05F11/00C05F11/08C12N1/14C12N1/16C12N1/20C12N1/145C12N1/205C12R2001/07C12R2001/645C12R2001/665C05F17/00
Inventor 张金萍李雪彬
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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