Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Meyerozyma guilliermondii and application thereof in biological deodorization

A biological deodorization and yeast technology, applied in the biological field, can solve the problems of secondary pollution, high equipment cost and high operation requirements, and achieve the effect of no biological hazard, high deodorization efficiency and obvious degradation effect

Active Publication Date: 2021-01-01
ZHEJIANG UNIV OF TECH
View PDF12 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods and chemical methods are suitable for occasions where the concentration of odor pollutants is high, and have the advantages of high odor treatment efficiency, short treatment residence time, and fast start-up; High disadvantage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Meyerozyma guilliermondii and application thereof in biological deodorization
  • Meyerozyma guilliermondii and application thereof in biological deodorization
  • Meyerozyma guilliermondii and application thereof in biological deodorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening and identification of Pichia pastoris

[0032] 1. Pick out bones, napkins and other substances from the kitchen waste in Yuxiu Canteen of Zhejiang University of Technology, and then use a crusher to smash the remaining waste.

[0033] 2. Take 1g of chopped kitchen waste and dilute it to 1×10 with ultrapure water. -6 , 1×10 -7 , 1×10 -8 , and take 200 μL of each dilution to solid YPD medium, spread evenly with a coating rod, and place it in a constant temperature incubator at 28°C for 48h to 72h, pick a single colony for streak purification and culture;

[0034] The formula of YPD medium is: yeast powder 10g, peptone 20g, glucose 20g, agar 15g.

[0035] 3. After purifying each single colony, select the strains with pure white color, smooth and shiny shape, no wrinkle, and slight wine smell, and pick the single colony to extract the genome with the FastDNA Spin Kit for Soil kit.

[0036] 4. Use the extracted genome as a template for PCR. The PCR sy...

Embodiment 2

[0038] The preparation of embodiment 2 biological deodorant bacteria

[0039] 1. The Pichia moniliformis strain screened in Example 1 was inserted into the YPD medium.

[0040] 2. Inoculate the activated strains into YPD liquid medium for shaking culture (37°C, 180r / min) for more than 12 hours.

[0041] 3. Count the cultured bacterial liquid, and adjust the bacterial content to 10 8 ~10 9 CFU / ml.

[0042] 4. Centrifuge the bacterial liquid (12000 r / min, 10 min), discard the supernatant after centrifugation, and collect the bacterial cells.

[0043] 5. Mix the bacterial cells with diatomite and corn stalks at a ratio of 1:1 (w / w), and then place them in a 28°C incubator to dry and constant weight, that is, various bacterial agents. Diatomite is a molecular-grade product purchased from the market; corn stalks are pre-treated and then mixed with bacterial agents. The pre-treatment steps are: firstly crush the raw materials of corn stalks into 80-100 mesh powder, wash and dry ...

Embodiment 3

[0048] Example 3 Carry out airtight deodorization test with kitchen waste as substrate

[0049] The fresh kitchen waste was crushed with a blender and divided into 500ml Erlenmeyer flasks. Each Erlenmeyer flask was divided into 100g (wet weight) of fresh food waste. Three parallel experiments were set up for each group of experiments. Then the bacterial agent prepared in Example 2 was added in an amount of 10g / bottle, and a blank control was set simultaneously. Then seal it with three layers of plastic wrap, and tie the mouth of the bottle tightly with a rubber band. Place in an incubator at 28°C, and measure the concentrations of ammonia and hydrogen sulfide after 24 hours.

[0050] After three groups of parallel experiments, it was found that the degradation rate of hydrogen sulfide in kitchen waste was 79.23%, and the degradation rate of ammonia gas was 25.71% after inoculation of biological deodorizing bacteria agent for 24 hours. From the above experimental results, it ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Meyerozyma guilliermondii K1 and an application thereof in biological deodorization, and belongs to the technical field of biology. The preservation number of the Meyerozyma guilliermondii K1 is CCTCC NO: M 2020191. The strain is high in deodorization efficiency and free of biological danger, and a biological deodorization microbial agent prepared from the Meyerozyma guilliermondii K1 has obvious degradation effects on odors generated by restaurant and kitchen waste, and has relatively good application potential.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a Pichia quaternary yeast (Meyerozymagilliermondii) K1 and its application in biological deodorization. Background technique [0002] Odor is a general term for all gases that stimulate the olfactory organs, cause unpleasantness and damage the living environment of human beings. Odor is one of the seven major public hazards recognized in the world, second only to noise. The stench will cause nausea, dizziness, vomiting and other reactions, and serious respiratory, cardiovascular and other diseases. Due to its high moisture content and high organic matter content, kitchen waste is easily spoiled, resulting in odor and breeding of insects and flies. [0003] At present, there are three main methods of deodorization: physical methods, chemical methods and biological methods. Physical methods and chemical methods are suitable for occasions with high concentration of odor po...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16B01D53/85C12R1/84
CPCC12N1/16B01D53/85B01D2251/95C12R2001/84C12N1/165Y02A50/20
Inventor 邹树平丁亦然薛亚平郑裕国周海岩柯霞
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com