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Meyerozyma guilliermondii for controlling postharvest diseases of pome

A post-harvest disease and yeast technology, which is used in the preservation of fruits and vegetables, microorganism-based methods, and food preservation, etc., to achieve the effects of broad antibacterial spectrum, reduced losses, and significant economic and social benefits.

Active Publication Date: 2017-12-12
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly four kinds of Pichia genus used in postharvest biological control of fruits, but there is no report on the application of Pichia guilliermondii in pome fruit

Method used

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  • Meyerozyma guilliermondii for controlling postharvest diseases of pome

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Effect test

Embodiment 1

[0023] Embodiment 1: the microbiological characteristics of Pichia pastoris (Meyerozyma guilliermondii) Y1 strain are as follows:

[0024] 1) Morphological features

[0025] (1) NYDA medium (beef extract 8g, yeast extract 5g, glucose 10g, agar 20g, 121°C moist heat sterilization for 20min) was cultured at 28°C for 48h, the colony was round, with smooth edges, moist and easy to provoke. The cells are oval in shape and scattered individually.

[0026] (2) After culturing in NYDB liquid medium for 24 hours, no mold was formed, and the bacterial solution was turbid with precipitation. Microscopically, the yeast cells were oval and budded.

[0027] 2) Molecular biological identification

[0028] Sequence analysis of the 5.8S rDNA-ITS region of the isolated and screened yeast strain Y1 was searched in GenBank and identified as Pichia guilliermondii. According to the retrieved homologous strains, the Mege5.1 program of DNAStar software was used to construct the biological evolutio...

Embodiment 2

[0030] Embodiment 2: Pichia mongolia Y1 safety experiment

[0031] 1) Experimental scheme

[0032] Preparation of Yeast Sludge and Total Determination

[0033] Pichia guilliermondii Y1 (Meyerozyma guilliermondii Y1) was inoculated in a Erlenmeyer flask containing NYDB medium, placed on a shaker, cultured at 28°C for 20 hours, and then centrifuged to obtain a fungus sludge. Gradiently dilute the bacteria slime with sterile water (10 -4 , 10 -5 , 10 -6 , 10 -7 ), and then pipette 100 μL of the diluted solution for uniform coating (NYDA), and count the experimental results after incubation. The whole experiment was performed in parallel twice. Each time the mice were gavaged with bacteria solution, they were diluted with fresh bacteria sludge.

[0034] The experimental subjects are ICR mice, provided by the Animal Experiment Center of Jiangsu University, the certificate number is NO.201613561, the license number is SCXK (Su) 2013-0011, and the implementation standard is GB...

Embodiment 3

[0040] Embodiment 3: the control effect of Pichia mongolica Y1 on Pythium penicillium

[0041] 1) Experimental scheme

[0042] Pichia mongolica Y1 was inoculated in a Erlenmeyer flask containing NYDB medium, cultured in a shaker at 180rpm and 28°C for 20h, centrifuged to obtain the bacteria, resuspended with sterile water and adjusted to a concentration of 1×10 8 cells / mL. Penicillium expansum (P.expansum) was cultured on PDA medium at 28°C for 7 days, the spores were washed with sterile water and the concentration of spores was adjusted to 1×10 5 spores / mL.

[0043] The fruits to be tested have been carefully selected, and the pears with uniform fruit, no scratches on the surface, no mechanical damage and no damage from diseases and insect pests are selected, and three uniform distributions are formed on the equator of the surface of the pome fruit with a burnt puncher. Wounds with consistent size and depth (5mm×3mm). Add 30 μL of the following treatment solution equally ...

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Abstract

The invention discloses meyerozyma guilliermondii for controlling postharvest diseases of pome, and belongs to the field of biological prevention and treatment for postharvest diseases of fruits. The preservation number of the identified meyerozyma guilliermondii is CCTCC NO:M 2017270. The meyerozyma guilliermondii is activated, cultured and centrifuged to obtain thalli when used, and the thalli are diluted by sterile water to prepare bacterium suspension with the concentration of 1.0*10<8>bacteria / mL. The pome is soaked in the bacterium suspension for approximately 1 min, then is subjected to natural air drying, is placed in plastic baskets and is stored under room-temperature conditions after being sealed by preservative films. The meyerozyma guilliermondii has the advantages that the penicillosis incidence and the natural rotting rate of the pome treated by the meyerozyma guilliermondii are obviously reduced, accordingly, the meyerozyma guilliermondii can be used for preventing and treating the postharvest diseases of the pome instead of chemical fungicides, harm on human due to the chemical fungicides can be prevented, and the meyerozyma guilliermondii has an obvious economic benefit and an obvious social benefit.

Description

technical field [0001] The invention relates to a strain of Pichia guilliermondii (Meyerozyma guilliermondii) Y1, which can control postharvest blue mold of pear fruit, and belongs to the field of biological control of postharvest fruit diseases. Background technique [0002] Pear (Pear) belongs to the Rosaceae plant. Pear fruit contains a variety of vitamins, protein, sugar, fat, crude fiber, calcium, phosphorus, iron and other minerals. Those who like it. At present, China is the country with the largest production and export volume of pome fruit in the world. The output in 2010 / 2011 accounted for 70% of the world's total output, and the export volume accounted for 30% of the world's total export volume. my country has a wide range of pear planting, from Heilongjiang in the north to Guangdong in the south, from Xinjiang in the west, to the seashore in the east, and there are various varieties of pears. However, the loss of pear fruits during storage, transportation and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155C12N1/145C12R2001/645
Inventor 张晓云杨红娟张红印严圆任晓锋赵利娜
Owner JIANGSU UNIV
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