64 results about "Lysine arginine" patented technology

This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, δ-hydroxylysine, and δ-hydroxy-ω-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.

The present invention addresses the problem of generalizing and improving a DNA-binding protein in which a PPR is used. Provided is a protein including at least one PPR motif having a structure represented by formula (1), wherein one PPR motif (Mn) included in the protein is a PPR motif having three amino acids of No. 1 A.A., No. 4 A.A., and No. ''ii'' (-2) A.A., in a combination of specific aminoacids corresponding to a target DNA base or a target DNA base sequence, and satisfies at least one selected from the group consisting of the following (a)-(h): (a) No. 7 A.A. in the PPR motif (Mn) isisoleucine (I); (b) No. 9 A.A. in the PPR motif (Mn) is alanine (A); (c) No. 10 A.A. in the PPR motif (Mn) is tyrosine (Y); (d) No. 18 A.A. in the PPR motif (Mn) is lysine (K), arginine (R), or histidine (H); (e) No. 20 A.A. in the PPR motif (Mn) is glutamic acid (E) or aspartic acid (D); (f) No. 29 A.A. in the PPR motif (Mn) is glutamic acid (E) or aspartic acid (D); (g) No. 31 A.A. in the PPR motif (Mn) is isoleucine (I); and (h) No. 32 A.A. in the PPR motif (Mn) is lysine (K), arginine (R), or histidine (H). (helix A)-X-(helix B)-L formula (1).

A composition of a long-acting enzyme comprises the enzyme in a formulation comprising a buffer and an additive selected from the group consisting of tranexamic acid, ε-aminocaproic acid, and analogs of L-lysine other than tranexamic acid and ε-aminocaproic acid, combinations thereof, and mixtures thereof. The composition can further comprise another additive selected from the group consisting of L-lysine, L-arginine, L-ornithine (or its pharmaceutically acceptable salts; e.g., L-ornithine hydrochloride), γ-aminobutyric acid, 5-aminovaleric acid, 7-aminoheptanoic acid, glycylglycine, triglycine, N-α-acetyl-L-arginine, betaine, sarcosine, gelatin, HSA, streptokinase, tPA, uPA, non-ionic surfactants, glycerin, D-sorbitol, combinations thereof, and mixtures thereof. A method for prolonging the activity of an autodegradable enzyme comprises storing the enzyme after manufacture at a low pH, and reconstituting the acidified enzyme before use with a solution containing at least one of such additives. The method is useful to provide enzyme for wide use, which otherwise would lose activity upon long storage. In one embodiment the method is applicable to provide enzyme for inducing controlled posterior vitreous detachment.