Improved L-15 culture medium and application thereof in culturing prawn muscle cells

Abstract

The invention discloses an improved L-15 culture medium and application thereof in culturing prawn muscle cells. The improved L-15 culture medium contains 10 mmol / L of alanine, 3 mmol / L of glycine, 3 mmol / L of serine, 1.5 mmol / L of histidine, 0.5 mmol / L of lysine, 1.5 mmol / L of arginine, 1.5 mmol / L of valine, 1 mmol / L of threonine, 0.5 mmol / L of methionine, 0.5 mmol / L of leucine, 5 mmol / L of aspartic acid, 0.5 mmol / L of glutamine and 1 mmol / L of phenylalanine. The oral liquid also contains taurine, proline, glutamic acid, fetal calf serum and double antibodies, and the pH value of the oral liquid is 7.2-7.4. According to the improved L-15 culture medium, the survival rate and the adherence rate of the primary muscle cells of the penaeus vannamei boone are increased, and the growth state of the cells is improved.

Description

technical field [0001] The invention relates to an improved L-15 medium and its application in culturing prawn muscle cells. Background technique [0002] The establishment of shrimp cell lines is the basis for studying the pathogenic mechanism of viruses, isolating and purifying shrimp viruses, and developing effective diagnostic reagents. The successful establishment of shrimp cell lines can not only precisely control the experimental conditions, but also greatly reduce the experimental cost and obtain better research results. The culture medium is an indispensable part of the cultured cells. The existing culture medium is not enough for the establishment of the shrimp cell line. Therefore, the optimization of the cell culture medium is one of the keys to the successful establishment of the shrimp cell line. This is of great significance for the study of the shrimp cell culture in vitro . [0003] Currently there is no cell culture medium specifically for prawns, and the...

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Problems solved by technology

[0005] However, the above-mentioned patent does not accurately determine the amino acid requirements of the cells, and only expands all components of the L-15 medium by 1.5 times

Moreover, the primary cell extraction method is tissue block culture, which requires several hours or even days of culture to obtain cells, and the tissue block culture method is more likely to be contaminated during the culture process

The medium working solution of this patent also added 20ng / mL basic fibroblast growth factor (bFGF) and 20ng / mL epidermal growth factor (EGF), which greatly increased the cost

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